[1]郭琪,徐珍珍,黄芳,等.棉花HKT基因家族的全基因组分析[J].江苏农业学报,2017,(05):975-981.[doi:doi:10.3969/j.issn.1000-4440.2017.05.003]
 GUO Qi,XU Zhen-zhen,HUANG Fang,et al.Genome-wide analysis of high-affinity potassium transporter gene family in cotton[J].,2017,(05):975-981.[doi:doi:10.3969/j.issn.1000-4440.2017.05.003]
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棉花HKT基因家族的全基因组分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年05期
页码:
975-981
栏目:
遗传育种·生理生化
出版日期:
2017-10-30

文章信息/Info

Title:
Genome-wide analysis of high-affinity potassium transporter gene family in cotton
作者:
郭琪1徐珍珍1黄芳2徐鹏1张香桂1沈新莲1
(1.江苏省农业科学院经济作物研究所/农业部长江下游棉花和油菜重点实验室,江苏南京210014;2.南通大学生命科学院,江苏南通226019)
Author(s):
GUO Qi1XU Zhen-zhen1HUANG Fang2XU Peng1ZHANG Xiang-gui1SHEN Xin-lian1
(1.Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and Rapeseed,Ministry of Agriculture, P.R.China,Nanjing 210014,China;2.School of Life Science,Nantong University,Nantong 226019,China)
关键词:
HKT基因家族生物信息棉花
Keywords:
HKT gene familybioinformaticscotton
分类号:
S562
DOI:
doi:10.3969/j.issn.1000-4440.2017.05.003
文献标志码:
A
摘要:
HKT(high-affinity K+transporter)转运蛋白在调节植物体内K+/Na+转运,参与逆境胁迫调控中发挥重要作用。本研究利用生物信息学方法,系统分析了棉花HKT基因家族的分类、系统进化树、组织表达以及HKT蛋白的亚细胞定位、跨膜结构、基序情况等。结果显示,棉花中共有8个HKT基因家族成员,编码的8个HKT蛋白都定位到了质膜上,具有 7~10个不等的跨膜数;系统进化树分析结果表明,棉花8个HKT基因家族成员大致分为2个小组,在进化树上分支比较近的HKT基因家族成员编码的HKT蛋白具有完全相同或者比较类似的motif类型和跨膜数;组织表达分析发现,克劳茨基棉仅有GrHKT1;1和GrHKT1;2 2个成员在200 mmol/L的NaCl溶液处理0 h、3 h、12 h、72 h和144 h后具有不同程度的响应。
Abstract:
High-affinity potassium transporter(HKT) is involved in K+/Na+transportation, and plays an important role in adaptation to abiotic stress. The classification of HKT gene family, HKT protein subcellular localization, transmembrane domain, and tissue expression pattern in cotton were systematically analyzed by bioinformatics in this study. Eight HKT genes were identified, and all proteins encoded were localized on the plasma membrane, with 7-10 transmembrane domains. Phylogenetic tree revealed that 8 HKT genes could be divided into 2 groups, and identical or similar motif type and transmembrane domain were found in closely-related branches. Only GrHKT1;1 and GrHKT1;2 were differentially expressed in Gossypium Klotzschianum subjected to 200 mmol/L NaCl treatment for 0 h, 3 h, 12 h, 72 h and 144 h.

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备注/Memo

备注/Memo:
收稿日期:2017-04-01 基金项目:国家转基因生物新品种培育重大专项(2016ZX08005-004-002);江苏省协同创新中心项目;江苏省农业科技自主创新基金项目[CX(14)2065];江苏省科技项目(BK20150540) 作者简介:郭琪(1987-),女,山西太原人,硕士,助理研究员,主要从事植物基因分子生物学研究. (Tel) 025-84390291;(E-mail)sevenguo86@163.com 通讯作者:沈新莲,(E-mail)xlshen68@126.com
更新日期/Last Update: 2017-11-03