[1]蔡志翔,沈志军,马瑞娟,等.桃同株叶片杂色材料的MSAP分析[J].江苏农业学报,2016,(03):662.[doi:10.3969/j.issn.1000-4440.2016.03.027]
 CAI Zhi-xiang,SHEN Zhi-jun,MA Rui-juan,et al.Analysis of leaf-variegated peach by methylation-sensitive amplification polymorphism[J].,2016,(03):662.[doi:10.3969/j.issn.1000-4440.2016.03.027]
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桃同株叶片杂色材料的MSAP分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年03期
页码:
662
栏目:
园 艺
出版日期:
2016-06-30

文章信息/Info

Title:
Analysis of leaf-variegated peach by methylation-sensitive amplification polymorphism
作者:
蔡志翔 沈志军 马瑞娟 严 娟 俞明亮
江苏省农业科学院园艺研究所,江苏省高效园艺作物遗传改良重点实验室,江苏 南京 210014
Author(s):
CAI Zhi-xiang SHEN Zhi-jun MA Rui-juan YAN Juan YU Ming-liang
Institute of Horticulture, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China
关键词:
嵌合体 DNA甲基化 甲基化敏感多态性扩增(MSAP)
Keywords:
Prunuspersica chimaera DNA methylation methylationsensitive amplification polymorphism(MSAP)
分类号:
S662.1
DOI:
10.3969/j.issn.1000-4440.2016.03.027
文献标志码:
A
摘要:
为探寻桃同株叶片杂色材料PCM-1的突变机制,建立了桃PCM-1G和PCM-1R DNA样品的基因池,用256对甲基化敏感扩增多态性(MSAP)选择性扩增引物进行全基因组甲基化MSAP分析。筛选出23对条带清晰、丰富且重复性好的引物组合。扩增出200~1 000 bp的位点397个,其中PCM-1G、PCM-1R两种类型半甲基化位点分别为38个和44个,分别占全部位点的9.6%和11.1%; 全甲基化位点分别为64个和67个,分别占全部位点的16.1%和16.9%; 总甲基化率分别为25.7%和28.0%。成功分离了29条甲基化修饰DNA序列,通过测序发现有22条能够得到桃基因组支持且同源性较高,其中2条来自于类胡萝卜素裂解双加氧酶4(CCD4)、3条来自于Omega-羟基棕榈酸阿魏酸转移酶; 推测CCD4和Omega-羟基棕榈酸阿魏酸转移酶可能与叶色嵌合突变有关。
Abstract:
To explore the mutation mechanism of leaf-variegated peach material PCM-1, a peach gene pool of the PCM-1G and PCM-1R DNA was established. 256 pairs of primers were used for genome-wide methylation sensitive amplification polymorphism(MSAP)analysis. The 23 pairs of primers, which amplified clear, rich and repeatable fragments, were selected to identify 397 loci in 200~1 000 bp. 44 hemimethylated DNA loci were in PCM-1R and 38 in PCM-1G, and 67 full methylated DNA loci were in PCM-1R and 64 in PCM-1G. The total methylation rates were 25.7% and 28.0% in PCM-1G and PCM-1R respectively. 29 methylated DNA sequences were successfully isolated from these loci, 22 of which could be supported by the peach genome with high homology. Two sequences out of 22 came from carotenoid cleavage dioxygenase 4(CCD4), and three from omega-hydroxypalmitate O-feruloyltransferase. It was speculated that CCD4 and omega-hydroxypalmitate O-feruloyltransferase were related with the leaf color chimeric mutation.

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备注/Memo

备注/Memo:
收稿日期:2015-10-28
基金项目:国家自然科学基金项目(31101517); 江苏省农业科技自主创新基金项目[CX(12)5034]
作者简介::蔡志翔(1979-),江苏南京人,硕士,助理研究员,从事桃种质资源研究。(E-mail)czx1y05@163.com;(Tel)025-84390855
更新日期/Last Update: 2016-06-30