[1]韩悦,李晓冰,周宏胜,等.微酸性电解水对桃果实采收后软腐病的防治效果[J].江苏农业学报,2025,(11):2146-2156.[doi:doi:10.3969/j.issn.1000-4440.2025.11.008]
　HAN Yue,LI Xiaobing,ZHOU Hongsheng,et al.Efficacy of slightly acidic electrolyzed water in controlling postharvest soft rot pathogen of peach[J].,2025,(11):2146-2156.[doi:doi:10.3969/j.issn.1000-4440.2025.11.008]
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微酸性电解水对桃果实采收后软腐病的防治效果()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年11期
页码:
2146-2156
栏目:
植物保护
出版日期:
2025-11-30

文章信息/Info

Title:
Efficacy of slightly acidic electrolyzed water in controlling postharvest soft rot pathogen of peach
作者:
韩悦1; 2; 3; 李晓冰1; 2; 3; 周宏胜2; 3; 张映曈2; 3; 胡花丽2; 3; 罗淑芬2; 3; 刘雪松2; 3; 刘欣页2; 3; 赵淳4; 李鹏霞2; 3; 凌军2; 3
(1.沈阳农业大学食品学院,辽宁沈阳110866；2.江苏省农业科学院农业设施与装备研究所,江苏南京210014；3.江苏省农业科学院/农业农村部农产品冷链物流技术重点实验室,江苏南京210014；4.东海县晶质农产品电商交易有限公司,江苏连云港222300)
Author(s):
HAN Yue1; 2; 3; LI Xiaobing1; 2; 3; ZHOU Hongsheng2; 3; ZHANG Yingtong2; 3; HU Huali2; 3; LUO Shufen2; 3; LIU Xuesong2; 3; LIU Xinye2; 3; ZHAO Chun4; LI Pengxia2; 3; LING Jun2; 3
(1.Food Science College, Shenyang Agriculture University, Shenyang 110866, China;2.Institute of Agricultural Facilities and Equipment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;3.Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cold Chain Logistics Technology for Agro-Products, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China;4.Donghai County Jingzhi Agricultural Products E-commerce Trading Co., Ltd., Lianyungang 222300, China)
关键词:
桃; 软腐病; 匍枝根霉; 微酸性电解水; 防治效果
Keywords:
peach; soft rot; Rhizopus stolonifer; slightly acidic electrolyzed water; control efficacy
分类号:
S436.621.1
DOI:
doi:10.3969/j.issn.1000-4440.2025.11.008
文献标志码:
A
摘要:
桃果实皮薄、肉质软,采后极易受到病原真菌侵染,引发病害尤其是引发的软腐病严重影响其贮藏寿命和市场价值。为了明确微酸性电解水(SAEW)对桃果实采收后软腐病防治的最佳浓度及处理时间,本研究通过分离、纯化并鉴定确定引起桃软腐病发生的病原菌为匍枝根霉(Rhizopus stolonifer)TF-1菌株,采用不同质量浓度的SAEW处理该病原菌菌株的孢子和菌丝并评估其抑制效果,结果显示,质量浓度100 mg/L SAEW处理5 min可有效降低菌株TF-1的孢子萌发率,抑制菌丝生长,并诱导菌丝细胞蛋白质和核酸外泄。碘化丙啶(PI)染色结果表明,100 mg/L SAEW处理5 min后9329%的孢子细胞膜受损,表明SAEW具有显著的杀菌作用。说明,SAEW可有效抑制匍枝根霉的生长,为桃果实采收后软腐病的防治提供了一种安全、经济且高效的杀菌剂。
Abstract:
Peach fruit, characterized by its thin skin and soft flesh, is highly susceptible to fungal infections after harvest. The resulting diseases, particularly soft rot, significantly reduce its storage life and market value. To identify the optimal concentration and treatment time of slightly acidic electrolyzed water (SAEW) for controlling postharvest soft rot in peach fruit, the causal pathogen was isolated, purified, and identified as the strain Rhizopus stolonifer TF-1. Spores and mycelia of the pathogen were treated with SAEW at different mass concentrations, and the inhibitory effects were assessed. The results showed that treatment with 100 mg/L SAEW for five minutes effectively reduced the spore germination rate of strain TF-1, inhibited mycelial growth, and induced the leakage of cellular proteins and nucleic acids. Propidium iodide (PI) staining revealed that 93.29% of spores exhibited compromised membrane integrity, demonstrating the significant bactericidal efficacy of SAEW. This study demonstrates that SAEW can effectively suppress the growth of Rhizopus stolonifer, highlighting its potential as a safe, cost-effective, and efficient fungicidal agent for managing postharvest soft rot in peach.

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备注/Memo

备注/Memo:
收稿日期：2024-12-22基金项目：国家重点研发计划项目(2022YFD2100601)；江苏省自然科学基金项目(BK20221434)；中央引导地方科技发展资金项目(BE2024014)作者简介：韩悦(1999-),女,辽宁抚顺人,硕士研究生,主要研究方向为果蔬采后保鲜。(E-mail)1545293919@qq.com通讯作者：李鹏霞,(E-mail)guoshubaoxian@163.com；凌军,(E-mail)robinlingjun@126.com
更新日期/Last Update: 2025-12-18