[1]朱道仙,陆江,赵学刚,等.大黄素通过选择性调节肠道菌群缓解犬慢性肾衰竭[J].江苏农业学报,2020,(06):1489-1497.[doi:doi:10.3969/j.issn.1000-4440.2020.06.019]
　ZHU Dao-xian,LU Jiang,ZHAO Xue-gang,et al.Alleviation of chronic renal failure in dogs through selectively regulation of gut microbiota by emodin[J].,2020,(06):1489-1497.[doi:doi:10.3969/j.issn.1000-4440.2020.06.019]
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大黄素通过选择性调节肠道菌群缓解犬慢性肾衰竭()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年06期
页码:
1489-1497
栏目:
畜牧兽医·水产养殖
出版日期:
2020-12-31

文章信息/Info

Title:
Alleviation of chronic renal failure in dogs through selectively regulation of gut microbiota by emodin
作者:
朱道仙1; 陆江2; 赵学刚2; 刘莉1; 吴双1; 3
(1.江苏农牧科技职业学院动物医学院，江苏泰州225300；2.江苏农牧科技职业学院宠物科技学院，江苏泰州225300；3.江苏省兽用生物制药高技术研究重点实验室，江苏泰州225300)
Author(s):
ZHU Dao-xian1; LU Jiang2; ZHAO Xue-gang2; LIU Li1; WU Shuang1; 3
(1.Department of Animal Medicine, Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China;2.Department of Pet Science and Technology, Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China；3.Jiangsu Provincial Key Laboratory of Veterinary Bio-pharmaceutical High-tech Research，Taizhou 225300，China)
关键词:
大黄素; 犬; 慢性肾衰竭; 肠道菌群; 16S rDNA测序
Keywords:
emodin; dog; chronic renal failure; gut microbiota; 16S rRNA sequencing
分类号:
S858.292.659
DOI:
doi:10.3969/j.issn.1000-4440.2020.06.019
文献标志码:
A
摘要:
为研究大黄素对犬慢性肾衰竭(CRF)的治疗效果与对肠道菌群的调节作用之间的关系，探究大黄素延缓CRF的作用机制，采用34例犬慢性肾衰竭病例，其中大黄素治疗组(RT)22例，常规治疗组(UT)12例。UT组患犬口服复方α-酮酸片+肾脏处方粮；RT组在UT组基础上按100 mg/(kg·d)口服大黄素，治疗期为45 d。定时采集患犬血液，检测尿毒素水平并无菌收集患犬粪便，用于肠道菌群16S rDNA测序及其代谢物检测。将2组治疗前后的患犬粪菌液移植给慢性肾衰竭模型无菌小鼠，验证患犬肠道菌群对CRF的影响。结果显示：RT组患犬的血清肌酐(Scr)、血尿素氮(BUN)、硫酸吲哚酚(IS)和硫酸对甲酚(PCS)含量逐渐降低，肠道菌群丰度增加，与试验前及UT组有显著差异；菌群结构也发生改变，表现为肠杆菌科、假单胞菌科等细菌丰度逐渐降低，而普氏杆菌属、乳杆菌属、丁酸梭菌属和双歧杆菌属等有益菌逐渐成为优势菌群，与UT组有差异。RT组肠道菌群在遗传信息处理、核苷酸代谢、辅助因子和维生素代谢等代谢途径方面表现活跃，患犬粪中代谢物丁酸含量升高，吲哚与H2S含量逐渐降低，与试验前及UT组有显著差异，这些代谢物含量的变化趋势与其生产菌群的变化趋势相一致。慢性肾衰竭无菌小鼠粪菌移植结果表明，RT组试验后的粪菌可以显著降低小鼠血中Scr和BUN含量。以上结果说明，大黄素可以使CRF犬紊乱的肠道菌群重建平衡，改变细菌代谢物水平，从而减少肠道菌群对犬慢性肾衰竭的不良影响。
Abstract:
To study the relationship between the therapeutic effect of emodin on chronic renal failure (CRF) and the regulating effect of emodin on gut microbiota in dogs, so as to explore the mechanism of emodin in alleviating CRF, 34 cases of CRF dogs were used, among which emodin treatment group (RT) contained 22 cases and usual treatment group (UT) contained 12 cases. The dogs in the UT group were given prescription diet containing compound α-ketoacid tablets plus kidney, while the dogs in the RT group were given 100 mg/(kg·d) emodin by oral on the basis of the prescription in the UT group, the treatment period was 45 days. Blood of the dogs was collected regularly to detect the level of urotoxin and feces of the dogs were collected aseptically for 16S rDNA sequencing of gut microbiota and related metabolites detection. The fecal bacterial solution of the two groups before and after treatment were transplanted into germ-less CRF mice respectively to verify the effect of gut microbiota on CRF. The results showed that the contents of serum creatinine (Scr), blood urea nitrogen (BUN), indole sulfate (IS) and p-cresol sulfate (PCS) of dogs in RT group decreased gradually and the abundance of gut microbiota increased, which showed significant difference with the group before test and UT group. The microflora structure also changed, presented as the abundance of Enterobacteriaceae, Pseudomonadaceae decreased gradually, while the beneficial bacteria such as Proteus, Lactobacillus, Butyricicoccus and Bifidobacterium gradually became the dominant microflora, which was different from UT group. The gut microbiota of RT group was active in metabolic pathways such as genetic information processing, nucleotide metabolism, cofactor and vitamin metabolism. The content of butyric acid in feces of the dogs from RT group increased, while the content of indole and H2S decreased gradually, which showed significant difference with the group before test and UT group. The change trend of these metabolites was consistent with the change trend of the productional flora. The result of fecal bacteria transplanting of the aseptic CRF mice showed that fecal bacteria from the RT group after experiment could significantly reduce the contents of Scr and BUN. In summary, emodin can restore the disarranged gut microbiota and change the levels of bacterial metabolites in CRF dogs, thus reduce the adverse effects of gut microbiota on CRF.

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备注/Memo

备注/Memo:
收稿日期：2020-04-18基金项目：江苏省333高层次人才培养工程或“六大人才高峰”项目(NY-009)；江苏农牧科技职业学院院级项目(NSF201706)；江苏省大学生创新创业训练计划项目(201812806015Y)作者简介：朱道仙(1978-)，女，安徽寿县人，硕士，讲师，主要从事动物临床营养及代谢病研究。 (Tel)13921700082;(E-mail)331802901@qq.com通讯作者：吴双，(E-mail)jswushuang@sina.cn
更新日期/Last Update: 2021-01-15