[1]裴徐梨,荆赞革,徐境,等.青花菜BoDof5.3基因的克隆及渍水胁迫表达特征分析[J].江苏农业学报,2020,(06):1498-1502.[doi:doi:10.3969/j.issn.1000-4440.2020.06.020]
　PEI Xu-li,JING Zan-ge,XU Jing,et al.Cloning and expression analysis of BoDof5.3 gene under waterlogging stress in broccoli[J].,2020,(06):1498-1502.[doi:doi:10.3969/j.issn.1000-4440.2020.06.020]
点击复制

青花菜BoDof5.3基因的克隆及渍水胁迫表达特征分析()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年06期
页码:
1498-1502
栏目:
园艺
出版日期:
2020-12-31

文章信息/Info

Title:
Cloning and expression analysis of BoDof5.3 gene under waterlogging stress in broccoli
作者:
裴徐梨1; 荆赞革1; 2; 徐境1; 唐征2; 宋波3
(1.昆明学院农学与生命科学学院，云南昆明650214；2.温州科技职业学院农业与生物技术学院，浙江温州325006；3.江苏省农业科学院蔬菜研究所，江苏南京210014)
Author(s):
PEI Xu-li1; JING Zan-ge1; 2; XU Jing1; TANG Zheng2; SONG Bo3
(1.College of Agriculture and Life Science, Kunming University, Kunming 650214, China;2.College of Agriculture and Biotechnology, Wenzhou Vocational College of Science and Technology, Wenzhou 325006, China;3.Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
青花菜; Dof基因; 基因克隆; 渍水胁迫; 表达特征
Keywords:
Brassica oleracea var. italica; Dof gene; gene clone; waterlogging stress; expression feature
分类号:
S635.3
DOI:
doi:10.3969/j.issn.1000-4440.2020.06.020
文献标志码:
A
摘要:
从青花菜中克隆得到一个Dof基因，命名为BoDof5.3。序列分析结果表明：该基因ORF全长774 bp，编码257个氨基酸，编码的蛋白质为亲水性蛋白质。青花菜BoDof5.3蛋白具有典型的Zf-Dof结构域，其二级结构以无规则卷曲为主。系统进化分析结果表明，青花菜BoDof5.3蛋白与甘蓝、花椰菜Dof蛋白亲缘关系最近，在进化树上聚为一组, 同属植物的Dof蛋白具有较近的亲缘关系。qRT-PCR分析结果显示，在渍水胁迫0 d到2 d时，BoDof5.3基因表达量呈下降趋势，6 d时表达量升高为对照的1.3倍，推测该基因可能参与青花菜渍水胁迫的响应。
Abstract:
In this study, a Dof gene named BoDof5.3 was cloned from broccoli. Sequence analysis results showed that this gene was 774 bp in length, which encoded 257 amino acids. Furthermore, the protein encoded by this gene was a hydrophilic protein. The BoDof5.3 contained a typical Zf-Dof domain, and its main secondary structure was the random coil. Results of phylogenetic analysis indicated that the BoDof5.3 protein of broccoli had the highest homology with the Dof protein of cabbage and cauliflower, and they were clustered into a group on phylogenetic tree. Furthermore, the Dof protein in the same genus had close relationship. The results of qRT-PCR showed that the expression level of BoDof5.3 decreased from 0 d to 2 d under waterlogging stress, and the expression level at six days of waterlogging stress was 1.3 times as much as that of control. It is speculated that BoDof5.3 gene may be involved in the response of broccoli to waterlogging stress.

参考文献/References:

[1]NOGUEROM, ATIF R M, OCHATT S, et al. The role of the DNA-binding one zinc finger (DOF) transcription factor family in plants [J]. Plant Science, 2013, 209(2): 32-45.
[2]WU Z M, CHENG J W, CUI J J, et al. Genome-wide identification and expression profile of Dof transcription factor gene family in pepper (Capsicum annuum L.) [J]. Frontiers in Plant Science, 2016, 7: 164.
[3]YANAGISAWA S. The Dof family of plant transcription factors [J]. Trends in Plant Science, 2002, 7(12): 555-560.
[4]LIJAVETZKY D, CARBONERO P, VICENTE-CARBAJOSA J. Genome-wide comparative phylogenetic analysis of the rice and Arabidopsis Dof gene families[J]. BMC Evolutionary Biology, 2003, 3(1): 17.
[5]GUO Y, QIU L J. Genome-Wide analysis of the Dof transcription factor gene family reveals soybean-specific duplicable and functional characteristics[J]. PLoS One, 2013, 8(9): e76809.
[6]MORENO-RISUENO M, MARTNEZ M, VICENTE-CARBAJOSA J. The family of DOF transcription factors: from green unicellular algae to vascular plants[J]. Molecular Genetics and Genomics, 2007, 277(4): 379-390.
[7]陈笑. 红花CtDof基因的克隆、表达分析及功能验证[D].长春: 长春师范大学, 2017.
[8]CHEN Y, CAO J. Comparative analysis of Dof transcription factor family in maize[J]. Plant Molecular Biology Reporter, 2015, 33(5): 1245-1258.
[9]张凯敏. 柽柳Dof转录因子基因克隆及耐盐功能分析[D].哈尔滨:东北林业大学, 2014.
[10]HENNIG L, GRUISSEM W, GROSSNIKLAUS U. Transcriptional programs of early reproductive stages in Arabidopsis[J]. Plant Physiology, 2004, 135(3):1765.
[11]裴徐梨,荆赞革,唐征,等. 青花菜花粉外壁蛋白基因的克隆与表达特征分析[J]. 基因组学与应用生物学, 2014, 33(5): 1046-1052.
[12]刘俊,黄容,程占超,等. 毛竹PheDof4-1基因克隆及表达分析[J]. 安徽农业大学学报, 2017, 44(3): 398-403.
[13]李小艳,裴徐梨,荆赞革,等. 青花菜肉桂酰辅酶A还原酶基因的克隆与表达特征分析[J].热带作物学报, 2016, 37(11): 2199-2203.
[14]王旭明，赵夏夏，陈景阳，等. 盐胁迫下水稻孕穗期SS和SPS活性与糖积累的响应及其相关性分析[J].江苏农业学报，2018，34(3)：481-486.
[15]李爱，孙汪亮，李林，等. 外源褪黑素对干旱胁迫下紫苏幼苗生长的影响[J]．江苏农业科学，2018，46(2)：70-73.
[16]关晓溪，隋常玲，胡海军，等. 弱光胁迫对烟草幼苗抗氧化系统及相关基因表达的影响[J].南方农业学报，2019, 50(5):957-963.
[17]李畅，苏家乐，刘晓青，等. 旱涝交替胁迫对杜鹃花生理特性的影响[J].江苏农业学报，2019,35(2):412-419.
[18]孙永娣，巢建国，谷巍，等. 汞胁迫对茅苍术光合特性及生理指标的影响[J].南方农业学报，2018，49(3):448-453.
[19]王霞霞，徐智明，朱德建，等. 5种抗生素对空心莲子草根系发育的影响[J].杂草学报，2018,36(1):8-13.
[20]曹睿哲，高世凯. 不同生育期旱涝交替胁迫对水稻产量及构成的影响[J].排灌机械工程学报，2019,37(7)：639-644.
[21]李辉,黄蔚,刘志薇,等. 茶树两个Dof转录因子的分离及其在温度胁迫中的响应分析[J].茶叶科学, 2016, 36(3): 312-322.
[22]CHEN Y, CAO J. Comparative analysis of Dof transcription factor family in maize[J]. Plant Molecular Biology Report, 2015, 33(5): 1245-1258.
[23]CORRALES A R, NEBAUER S G, CARRILLO L, et al. Characterization of tomato cycling Dof factors reveals conserved and new functions in the control of flowering time and abiotic stress responses[J]. Journal of Experimental Botany, 2014, 65(4): 995-1012.
[24]CAI X F, ZHANG Y Y, ZHANG C J, et al. Genome-wide analysis of plant-specific Dof transcription factor family in tomato[J]. Journal of Integrative Plant Biology, 2013, 55(6): 552-566.
[25]江海洋,骆晨,江腾,等. 玉米Dof转录因子家族基因的全基因组分析[J]. 生物信息学, 2010, 8(3); 198-201.
[26]SHAW L M, MCINTYRE C L, GRESSHOFF P M, et al. Members of the Dof transcription factor family in Triticum aestivum are associated with light-mediated gene regulation[J]. Functional & Integrative Genomics, 2009(9)： 485-498.
[27]MA J, LI M Y, WANG F, et al. Genome-wide analysis of Dof family transcription factors and their responses to abiotic stresses in Chinese cabbage[J]. BMC Genomics, 2015, 16: 33.
[28]HUANG W, HUANG Y, LI M. Dof transcription factors in carrot: genome-wide analysis and their response to abiotic stress [J]. Biotechnology Letters, 2016, 38: 145.

相似文献/References:

[1]朱长志,张志仙,檀国印,等.青花菜主要农艺性状配合力遗传分析[J].江苏农业学报,2016,(02):430.[doi:10.3969/j.issn.1000-4440.2016.02.029]
　ZHU Chang-zhi,ZHANG Zhi-xian,TAN Guo-yin,et al.Genetic analysis of combining ability of major agronomic traits in Brassica oleracea L. var. italica[J].,2016,(06):430.[doi:10.3969/j.issn.1000-4440.2016.02.029]
[2]唐跃辉,包欣欣,王健,等.小桐子Dof基因家族生物信息学与表达分析[J].江苏农业学报,2019,(01):15.[doi:doi:10.3969/j.issn.1000-4440.2019.01.003]
　TANG Yue-hui,BAO Xin-xin,WANG Jian,et al.Bioinformatics and expression analysis of the Dof gene family in physic nut[J].,2019,(06):15.[doi:doi:10.3969/j.issn.1000-4440.2019.01.003]
[3]王新亮,彭玲,王健,等.苹果Dof转录因子生物信息学及其表达分析[J].江苏农业学报,2021,(02):480.[doi:doi:10.3969/j.issn.1000-4440.2021.02.026]
　WANG Xin-liang,PENG Ling,WANG Jian,et al.Bioinformatics and expression analysis of the Dof transcription factors in apple[J].,2021,(06):480.[doi:doi:10.3969/j.issn.1000-4440.2021.02.026]
[4]高颖,杨亚苓,李慕紫,等.青花菜WRI4基因的克隆、生物信息学分析及表达载体的构建[J].江苏农业学报,2021,(03):710.[doi:doi:10.3969/j.issn.1000-4440.2021.03.021]
　GAO Ying,YANG Ya-ling,LI Mu-zi,et al.Cloning, bioinformatic analysis and expression vector construction of broccoli WRI4 gene[J].,2021,(06):710.[doi:doi:10.3969/j.issn.1000-4440.2021.03.021]

备注/Memo

备注/Memo:
收稿日期：2020-03-21基金项目：浙江省自然科学基金项目(LY18C150006)；云南省地方本科高校(部分)基础研究联合专项资金项目(2018FH001-044)；云南省基础研究计划青年项目(2019-1-C-25318000002236)；浙江省科技厅项目(2016C051-5-3)；温州市科技项目(2019ZX007-2)作者简介：裴徐梨(1990-)，女，云南个旧人，博士，讲师，主要从事蔬菜分子生物学研究。(E-mail)xuliP1990@163.com通讯作者：荆赞革，(E-mail)jingzange@aliyun.com
更新日期/Last Update: 2021-01-15