[1]唐跃辉,包欣欣,王健,等.小桐子Dof基因家族生物信息学与表达分析[J].江苏农业学报,2019,(01):15-25.[doi:doi:10.3969/j.issn.1000-4440.2019.01.003]
　TANG Yue-hui,BAO Xin-xin,WANG Jian,et al.Bioinformatics and expression analysis of the Dof gene family in physic nut[J].,2019,(01):15-25.[doi:doi:10.3969/j.issn.1000-4440.2019.01.003]
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小桐子Dof基因家族生物信息学与表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年01期
页码:
15-25
栏目:
遗传育种·生理生化
出版日期:
2019-02-26

文章信息/Info

Title:
Bioinformatics and expression analysis of the Dof gene family in physic nut
作者:
唐跃辉1; 包欣欣2; 王健1; 冯荆城3; 张梦3; 张慧聪3; 刘梦兰3
（1.周口师范学院植物遗传与分子育种重点实验室，河南周口466001；2.周口师范学院新闻与传媒学院，河南周口466001；3.周口师范学院生命科学与农学学院，河南周口466001）
Author(s):
TANG Yue-hui1; BAO Xin-xin2; WANG Jian1; FENG Jing-cheng3; ZHANG Meng3
(1.Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou 466001, China;2.School of Journalism and Communication, Zhoukou Normal University, Zhoukou 466001, China;3.College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou 466001, China)
关键词:
小桐子; Dof基因; 转录因子; 表达模式
Keywords:
physic nut (Jatropha curcas L.); Dof gene; transcription factor; expression pattern
分类号:
S794.3
DOI:
doi:10.3969/j.issn.1000-4440.2019.01.003
文献标志码:
A
摘要:
Dof 转录因子在植物的生长发育和非生物胁迫响应中起重要的调控作用。在本研究中，总共24个小桐子Dof基因被鉴定。保守域分析结果表明，小桐子Dof蛋白的结构域均含有CX2CX21CX2C基序。染色体定位分析结果表明， 23个Dof基因不均匀地分布在9个连锁群（LGs）上。系统发育树表明，24个小桐子Dof蛋白被分成9个组。在这24个Dof基因中，大多数在被检测的组织中显示出差异表达；14个Dof基因的表达在至少1个胁迫（干旱或盐胁迫）条件下显示出增加或者降低。在这14个差异表达Dof基因中，8个Dof基因（JcDof4、 JcDof8、 Jcdof9、 Jcdof10、 Jcdof11、 Jcdof21 Jcdof22、 Jcdof24）在至少1个处理时间点表现出对干旱和盐胁迫响应，2个基因（JcDof2、 JcDof23）仅对盐胁迫响应，4个基因（JcDof3、 JcDof5、 JcDof17、 JcDof20）仅对干旱胁迫响应。该结果将为进一步研究 Dof 基因在调控小桐子生长发育和非生物胁迫响应中的作用提供一些有价值的信息。
Abstract:
The Dof transcription factors play an important regulatory role in plant growth and development and abiotic stress response. In this study, a total of 24 Dof genes were identified in the physic nut. The results of conserved domain analysis showed that physic nut Dof proteins contained CX2CX21CX2C motif. Chromosomal location analysis results suggested that 23 Dof genes were unevenly distributed on nine linkage groups (LGs) of physic nut. Phylogenetic analysis results suggested that the 24 physic nut Dof proteins were clustered into nine groups. Most of these 24 Dof genes showed differential expression in these tested tissues. The expression of 14 Dof genes increased or decreased under at least one stress (drought or salt stress). Out of these 14 differentially expressed genes, eight Dof genes (JcDof4, JcDof8, Jcdof9, Jcdof10, Jcdof11, Jcdof21 Jcdof22, Jcdof24) showed the differential response to both drought and salt stress at least one time point, two Dof genes (JcDof2, JcDof23) only responded to salt stress, and four Dof genes (JcDof3, JcDof5, JcDof17, JcDof20) only responded to drought stress. The results will provide some valuable information for further studies on the roles of Dof genes in regulating physic nut’s growth, development and responses to abiotic stress.

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备注/Memo

备注/Memo:
收稿日期：2018-05-22 基金项目：河南省科学技术厅科技攻关项目（182102110200）；2018年度河南省高等学校重点科研项目（18A180035）；周口师范学院高层次引进人才科研基金启动项目（ZKNUC2016030、ZKNUC2015121） 作者简介：唐跃辉(1985-)，男，河南许昌人，博士，讲师，主要从事小桐子和水稻基因功能研究。（Tel）0376-8178586;（E-mail）yhtang2005@163.com。包欣欣与王健为共同第一作者。
更新日期/Last Update: 2019-02-27