[1]姚悦梅,孙德举,李建斌,等.低温对羽衣甘蓝幼苗氮代谢的影响[J].江苏农业学报,2019,(05):1191-1196.[doi:doi:10.3969/j.issn.1000-4440.2019.05.027]
 YAO Yue-mei,SUN De-ju,LI Jian-bin,et al.Effects of low temperature on nitrogen metabolism in kale seedlings[J].,2019,(05):1191-1196.[doi:doi:10.3969/j.issn.1000-4440.2019.05.027]
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低温对羽衣甘蓝幼苗氮代谢的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年05期
页码:
1191-1196
栏目:
园艺
出版日期:
2019-10-31

文章信息/Info

Title:
Effects of low temperature on nitrogen metabolism in kale seedlings
作者:
姚悦梅1孙德举1李建斌2山溪1张振超1戴忠良1
(1.江苏丘陵地区镇江农业科学研究所,江苏句容212400;2.江苏省农业科学院蔬菜研究所,江苏南京210014)
Author(s):
YAO Yue-mei1SUN De-ju1LI Jian-bin2SHAN Xi1ZHANG Zhen-chao1DAI Zhong-liang1
(1.Zhenjiang Institute of Agricultural Sciences of the Ning-Zhen Hilly District, Jurong 212400, China;2.Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
羽衣甘蓝低温胁迫氮代谢
Keywords:
kalelow temperature stressnitrogen metabolism
分类号:
S635.9
DOI:
doi:10.3969/j.issn.1000-4440.2019.05.027
文献标志码:
A
摘要:
以不同叶色、叶形、耐寒性的羽衣甘蓝幼苗(Y3、Y4、Y5、Y7)为试材,研究了不同时间低温(4 ℃)处理对羽衣甘蓝幼苗氮代谢的影响。结果表明,随着低温处理时间的延长,植株中可溶性蛋白质含量、脯氨酸含量、不同形态氮含量及相关酶活性都有不同程度的变化。随低温处理时间的延长,植株中硝态氮含量呈降低的趋势,但铵态氮含量呈增加的趋势,氮代谢相关酶活性均有不同程度的降低。但是当植株中谷氨酰胺合成酶(GS)/谷氨酸合酶(GOGAT)循环受到损伤时,谷氨酸脱氢酶(GDH)可能会参与NH+4的同化,从而完成铵同化。总之,在低温胁迫下,羽衣甘蓝幼叶中氮代谢受到不同程度的损伤,这可能会影响植株后期的生长。
Abstract:
The effects of low temperature treatment (4 ℃) on the nitrogen metabolism in Brassica oleracea seedlings (Y3, Y4, Y5, Y7) with different leaf color, leaf shape and cold tolerance were studied. The results showed that the contents of soluble protein, proline and different forms of nitrogen and the activity of related enzymes changed with the prolongation of low temperature treatment time. With the extension of low temperature treatment time, the content of nitrate nitrogen decreased, the content of ammonium nitrogen increased, and the activity of enzymes related to nitrogen metabolism decreased in varying degrees. However, when the glutamine synthetase (GS)/glutamate synthase (GOGAT) cycle in the plant was damaged, glutamic acid dehydrogenase (GDH) would participate in the assimilation of NH+4 and complete ammonia assimilation. In a word, nitrogen metabolism in young leaves of Brassica oleracea is damaged in different degree under low temperature stress, which may affect the growth of plant in later stage.

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备注/Memo

备注/Memo:
收稿日期:2019-04-08 基金项目:江苏省农业科技自主创新基金项目[CX(18)3075] 作者简介:姚悦梅(1974-),女,新疆石河子人,本科,副研究员,主要从事羽衣甘蓝种质创新利用与栽培技术研究工作,(E-mail)1045714975@qq.com 通讯作者:戴忠良,(E-mail)daizhongliang2008@126.com
更新日期/Last Update: 2019-11-11