[1]范军强,路晓明,王会文,等.低温胁迫下甘蓝型冬油菜抗寒性与叶片激素含量的关联性[J].江苏农业学报,2023,(01):15-21.[doi:doi:10.3969/j.issn.1000-4440.2023.01.002]
　FAN Jun-qiang,LU Xiao-ming,WANG Hui-wen,et al.Association between cold resistance and leaf hormone content of Brassica napus L. under low temperature stress[J].,2023,(01):15-21.[doi:doi:10.3969/j.issn.1000-4440.2023.01.002]
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低温胁迫下甘蓝型冬油菜抗寒性与叶片激素含量的关联性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年01期
页码:
15-21
栏目:
遗传育种·生理生化
出版日期:
2023-02-28

文章信息/Info

Title:
Association between cold resistance and leaf hormone content of Brassica napus L. under low temperature stress
作者:
范军强1; 2; 路晓明1; 2; 王会文1; 2; 武军艳1; 2; 刘丽君2; 马骊2; 蒲媛媛1; 2; 李学才1; 2; 孙万仓1; 2
（1.甘肃农业大学农学院,甘肃兰州730070；2.省部共建干旱生境作物学国家重点实验室,甘肃兰州730070)
Author(s):
FAN Jun-qiang1; 2; LU Xiao-ming1; 2; WANG Hui-wen1; 2; WU Jun-yan1; 2; LIU Li-jun2; MA Li2; PU Yuan-yuan1; 2; LI Xue-cai1; 2; SUN Wan-cang1; 2
(1.College of Agronomy,Gansu Agricultural University,Lanzhou 730070, China;2.State Key Laboratory of Aridland Crop Science, Lanzhou 730070, China)
关键词:
甘蓝型冬油菜; 低温胁迫; 半致死温度; 内源激素; 抗寒性
Keywords:
Brassica napus L.; low temperature stress; Semi-lethal temperature; Endogenous auxin; cold resistance; Correlation analysis
分类号:
S565.401
DOI:
doi:10.3969/j.issn.1000-4440.2023.01.002
文献标志码:
A
摘要:
为探究不同抗寒性甘蓝型冬油菜在应对低温胁迫时内源激素含量的变化,并阐明其抗寒性与叶片激素含量的关联性,选用感温性差异较大的4个甘蓝型冬油菜品种（品系）,分别于4 ℃及0 ℃处理不同时间后测定植株叶片内玉米素（Zeatin,ZT）、赤霉素（Gibberellin A3,GA3）、水杨酸（Salicylic acid,SA）、生长素（Indole-3-acetic acid,IAA）和脱落酸（Abscisic acid,ABA）5种内源激素的含量,并联系抗寒性评价指标进行相关性分析。结果表明,与对照（24 ℃）相比，低温处理后甘蓝型冬油菜叶片中IAA、ZT、GA3含量显著降低,ABA含量较对照（24 ℃）上升2～3倍,不同品种（品系）SA含量增幅差异较大。相关性分析结果表明,ABA、IAA、GA3、SA含量的耐寒系数与抗寒性呈显著负相关。总之,内源激素含量会随外界温度变化而变化,且强抗寒性甘蓝型冬油菜品种（品系）叶片中激素含量的变化（上升或下降）幅度小于弱抗寒性品种（品系）。
Abstract:
To study the changes of endogenous plant hormone content in Brassica napus L. varieties (strains) with different cold resistance under low temperature stress, and to clarify the correlation between their cold resistance and leaf hormone content, four B. napus L. varieties (strains) with great difference in temperature sensitivity were selected and were treated at 4 ℃, 0 ℃ for different times, respectively. Then contents of five endogenous hormones in the leaves of the plants were detected, such as zeatin (ZT), gibberellin A3 (GA3), salicylic acid (SA), indole-3-acetic acid (IAA) and abscisic acid (ABA), and correlation analysis was made with evaluation indexes of cold resistance. The results showed that, compared with the control (24 ℃), contents of IAA, ZT and GA3 in leaves of B. napus L. decreased significantly after low temperature treatment, and content of ABA increased by two to three times. The increase amplitude of SA content in different varieties (strains) of B. napus L. was different. Results of correlation analysis showed that, the cold resistance coefficients of ABA, IAA, GA3 and SA contents were significantly negatively correlated with cold resistance. In conclusion, the endogenous hormone content changes with the change of external temperature, and the change (increase or decrease) of hormone content in leaves of B. napus L. varieties (strains) with strong cold resistance was less than that of varieties(strains) with weak cold resistance.

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备注/Memo

备注/Memo:
收稿日期：2022-04-20基金项目：甘肃农业大学干旱生境作物学重点实验室主任基金项目（GSCS-2020-Z1）；国家现代农业产业技术体系项目(CARS-12)；国家自然科学基金项目(31860388、31960435)作者简介：范军强（1998-）,男,甘肃通渭人,硕士研究生,研究方向为作物遗传育种。（E-mail)2260651693@qq.com通讯作者：孙万仓,（E-mail）18293121851@163.com
更新日期/Last Update: 2023-03-21