[1]曹玉杰,薄凯亮,程春燕,等.外源尸胺增强黄瓜幼苗耐冷性的生理机制[J].江苏农业学报,2015,(01):122-129.[doi:10.3969/j.issn.1000-4440.2015.01.019]
　CAO Yu-jie,BO Kai-liang,CHENG Chun-yan,et al.Physiological mechanism of exogenous cadaverine enhancing the chilling tolerance of cucumber seedlings[J].,2015,(01):122-129.[doi:10.3969/j.issn.1000-4440.2015.01.019]
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外源尸胺增强黄瓜幼苗耐冷性的生理机制()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年01期
页码:
122-129
栏目:
园艺
出版日期:
2015-02-28

文章信息/Info

Title:
Physiological mechanism of exogenous cadaverine enhancing the chilling tolerance of cucumber seedlings
作者:
曹玉杰; 薄凯亮; 程春燕; 钱春桃; 陈劲枫
（南京农业大学园艺学院，作物遗传与种质创新国家重点实验室，江苏南京210095）
Author(s):
CAO Yu-jie; BO Kai-liang; CHENG Chun-yan; Qian Chun-tao; CHEN Jin-feng
（College of Horticulture, Nanjing Agricultural University / State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, China）
关键词:
黄瓜; 外源尸胺; 低温胁迫; 生理生化指标; 叶绿素荧光参数
Keywords:
cucumber; exogenous Cad; low temperature stress; physiological-biochemical index; chlorophyll fluorescence parameter
分类号:
S642.2
DOI:
10.3969/j.issn.1000-4440.2015.01.019
文献标志码:
A
摘要:
以黄瓜耐冷材料长春密刺、冷敏材料北京截头为试材，利用人工气候箱进行低温（12 ℃/7 ℃,昼/夜）及恢复处理，研究低温胁迫下外源尸胺（Cad）对黄瓜幼苗电解质渗透率、丙二醛（MDA）含量、可溶性蛋白质含量、可溶性糖含量、脯氨酸含量、超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性、过氧化氢酶（CAT）活性、抗坏血酸过氧化物酶（APX）活性及叶绿素荧光参数的影响。与对照相比，Cad 处理可以使低温胁迫下长春密刺、北京截头的电解质渗透率分别降低18.61%、9.54%，MDA含量分别降低13.13%、12.54%，可溶性蛋白质含量分别增加16.99%、11.20%，可溶性糖含量分别提高9.35%、2.90%，脯氨酸含量分别上升20.54%、17.36%；使SOD、POD、CAT、APX的活性均显著上升，分别提高9.64%和8.42%、19.83%和18.97%、31.95%和41.29%、10.66% 和17.13%；同时可改善幼苗叶绿素荧光参数的变化。在低温胁迫条件下，外源尸胺通过促进黄瓜幼苗叶片可溶性物质含量的增加及抗氧化酶活性水平的提高，来降低膜脂过氧化水平，从而增强黄瓜幼苗的抗冷性。
Abstract:
Cucumber (Cucumis sativus L.) variety Changchun mici (chilling-tolerant) and Beijing jietou ( chilling-sensitive ) were sprayed with cadaverine (Cad) and were then subjected to low temperature of 12 ℃/7 ℃ ( day/night ) and recovering treatment in the artificial climate chamber. The effects of Cad on the changes in electrolyte leakage rate, malondialdehyde (MDA) content, soluble protein content, soluble sugar content, proline content, superoxide dismutase (SOD) activity, peroxidase (POD) activity and catalase (CAT) activity, ascorbate peroxidase (APX) activity and chlorophyll fluorescence parameters under low temperature stress in the seedling leaves were investigated. Compared with the control treatment(water), Cad pretreatment could significantly reduce electrolyte leakage of Changchun mici and Beijing jietou by 18.61% and 9.54% respectively and MDA contents by 13.13%, 12.54% respectively, whereas increase soluble protein contents by 16.99% and 11.20% respectively, soluble sugar contents by 9.35% and 2.90% respectively, proline contents by 20.54% and 17.36% respectively, and promote the activities of SOD, POD, CAT, and APX by 9.64% and 8.42%, 19.83% and 18.97%, 31.95% and 41.29%, and 10.66% and 17.13%, as well as improve the changes of chlorophyll fluorescence parameters in cucumber seedling leaves under low temperature stress. The application of exogenous Cad could improve chilling tolerance in cucumber seedlings by decreasing the membrane lipid peroxidation, increasing soluble substances content and enhancing the activities of antioxidant enzymes.

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备注/Memo

备注/Memo:
收稿日期：2014-05-19 基金项目：国家重点基础研究发展计划项目（2009CB119001-01、2012CB113900）；“863”计划专项（2010AA10A108、2012AA100202）；江苏省科技支撑计划项目（BE2009310）；江苏省农业科技自主创新基金项目[CX(11)1002] 作者简介：曹玉杰(1984-),女,山东潍坊人,硕士研究生,主要从事蔬菜遗传育种与生物技术研究。 (E-mail)caoyujie08@163.com 通讯作者：陈劲枫,( E-mail)jfchen@njau.edu.cn
更新日期/Last Update: 2015-02-28