[1]张曼,胡雪丹,徐锦华,等.葫芦砧木种质资源耐冷性评价[J].江苏农业学报,2016,(06):1390-1395.[doi:doi:10.3969/j.issn.1000-4440.2016.06.030]
　ZHANG Man,HU Xue-dan,XU Jin-hua,et al.Evaluation on seedling cold tolerance of bottle gourd rootstock accessions[J].,2016,(06):1390-1395.[doi:doi:10.3969/j.issn.1000-4440.2016.06.030]
点击复制

葫芦砧木种质资源耐冷性评价()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年06期
页码:
1390-1395
栏目:
园艺
出版日期:
2017-02-07

文章信息/Info

Title:
Evaluation on seedling cold tolerance of bottle gourd rootstock accessions
作者:
张曼1; 胡雪丹12; 徐锦华1; 刘广1; 姚协丰1; 李苹芳1; 任润生1; 羊杏平1
(1. 江苏省农业科学院蔬菜研究所/江苏省高效园艺作物遗传改良重点实验室，江苏南京210014；2. 扬州大学园艺与植物保护学院，江苏扬州225000）
Author(s):
ZHANG Man1; HU Xue-dan12; XU Jin-hua1; LIU Guang1; YAO Xie-feng1; LI Ping-fang1; REN Run-sheng1; YANG Xing-ping1
(1. Institute of Vegetable, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China;2. School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225000, China)
关键词:
葫芦砧木; 低温胁迫; 生理生化指标; 耐冷性评价
Keywords:
Lagenaria siceraria (Molina) Standl.; cold stress; physiological and biochemical indices; evaluation of cold tolerance
分类号:
S642.9
DOI:
doi:10.3969/j.issn.1000-4440.2016.06.030
文献标志码:
A
摘要:
为筛选适宜低温环境的耐冷葫芦砧木品种，本研究以11份葫芦砧木种质资源为材料，分析其在8 ℃低温胁迫下的冷害指数，测定与耐冷性相关的7项生理生化指标，利用隶属函数法和聚类分析对其耐冷性进行综合评价。结果表明，低温处理下，冷害指数、抗氧化酶（SOD、CAT）活性、氧化损伤指标（MDA含量）、渗调物质（脯氨酸、可溶性蛋白）含量，可作为苗期耐冷性鉴定的评价指标。通过隶属函数法和聚类分析将11份供试葫芦砧木材料分为3大类群：强耐冷性品种（NZ、CF、AS、RS、HG和QY）、中度耐冷性品种（JX、Y1、JZ和Y3）和冷敏感性品种（KC）。强耐冷葫芦砧木NZ、CF、AS、RS、HG和QY可以作为耐冷葫芦砧木资源种质改良的候选材料。
Abstract:
This work aims to select cold tolerance bottle gourd rootstock varieties. Eleven bottle gourd rootstock accessions were used to analyze their cold tolerant ability. Chilling injury index and seven physiological and biochemical indices that were related to cold tolerance were evaluated. The cold tolerant ability of 11 bottle gourd germplasms was comprehensively evaluated by the methods of subordinate function value and cluster analysis. Chilling injury index, antioxidant enzymes (SOD, CAT), oxidative damage parameter (MDA content), osmolytes (Pro and soluble protein content) had significant correlation with seedling’s cold tolerant ability, which meaning that these six physiological and biochemical indices could be used as an effective assessment index for the cold tolerant ability of bottle gourd rootstock seedling. The cold tolerant ability of analyzed bottle gourd samples was classified into three levels by using the subordinate function and the cluster analysis: strong cold tolerance varieties (NZ, CF, AS, RS, HG and QY), intermediated cold tolerance varieties (JX, Y1, JZ and Y3) and cold sensitive variety (KC). NZ, CF, AS, RS, HG and QY were cold-tolerant bottle gourd rootstock varieties, which could be used as candidate materials for improvement of cold tolerant bottle gourd rootstocks.

参考文献/References:

[1]刘广, 羊杏平, 侯茜, 等. 2014年西瓜甜瓜砧木育种领域国内外研究进展[J]. 江西农业学报, 2015, 27(9): 11-16.
[2]MURATA J, OHARA K. Prevention of watermelonFusarium wilt by grafting Lagenaria[J]. Jpn J Phytopathol, 1936, 6:183-189.
[3]YETISIR H, SARI N. Effect of different rootstock on plant growth, yield and quality of watermelon[J]. Aust J Exp Agr, 2003, 43:1269-1274.
[4]LIN K H, KUO W S, CHIANG C M, et al. Study of sponge gourd ascorbate peroxidase and winter squash superoxide dismutase under respectiveooding and chilling stresses[J]. Sci Hortic, 2013, 162:333-340.
[5]卢昱宇，冯伟民，陈罡，等． 蔬菜嫁接技术研究进展及应用[J]． 江苏农业科学，2014，42(7):167-169．
[6]李瑶. 嫁接提高甜瓜耐低温生理机制的研究[D]. 长春：吉林大学，2014.
[7]王文英，刘喜存，董彦琪，等． 不同生理指标对几个砧木嫁接西瓜效果的影响[J]． 江苏农业科学，2015，43(8):144-145．
[8]孙吉庆, 孙令强, 李敏. 低温胁迫对砧木南瓜F1代及其亲本幼苗的影响[J]. 北方园艺, 2013（2）:20-22.
[9]徐光东, 张春沂, 陶士会, 等. 不同甜瓜砧木抗冷性鉴定试验[J]. 山东农业科学, 2012, 44(7):32-33, 36.
[10]王金茹. 甜瓜耐低温砧木的筛选及嫁接对品质的影响[D]. 长春：吉林大学, 2014.
[11]许勇, 王永健, 张峰, 等. 西瓜幼苗耐低温研究初报[J]. 华北农学报, 1997, 12(2): 93-96.
[12]张志良，翟伟菁. 植物生理学实验指导[M].3版. 北京：高等教育出版社, 2003.
[13]箫浪涛, 王三根. 植物生理学试验技术[M]. 北京: 中国农业出版社，2005:110.
[14]周广生, 梅方竹, 周竹青, 等. 小麦不同品种耐湿性生理指标综合评价及其预测[J]. 中国农业科学, 2003, 36(11):1378-1382.
[15]姜振升, 孙晓琦, 艾希珍, 等. 低温弱光对黄瓜幼苗Rubisco与Rubisco活化酶的影响[J]. 应用生态学报, 2010, 21(8):2045-2050.
[16]毕焕改, 王美玲, 姜振升, 等. 亚适温弱光对黄瓜幼苗光合酶活性和基因表达的影响[J]. 应用生态学报, 2011, 22(11):2894-2900.
[17]YANG G, ZOU H D, WU Y, et al. Identification and characterization of candidate genes involved in chilling responses in maize (Zea mays L.) [J]. Plant Cell Tiss Org, 2011, 106:127-141.
[18]HUANG M, GUO Z. Responses of antioxidative system to chilling stress in two rice cultivars differing in sensitivity[J]. Biol Plantarum, 2005, 9(1):81-84.
[19]VICTORIA B, OMAR B, PEDRO D, et al. Response to photoxidative stress induced by cold injaponica rice is genotype dependent[J]. Plant Sci, 2011, 180(5)：726-732.
[20]李晓明. 黄瓜耐冷指标的筛选及Ca2+在黄瓜耐冷中作用的研究[D]. 南京：南京农业大学，2006.
[21]曾亚文, 李绅崇, 普晓英，等. 云南稻核心种质苗期耐冷性及其地理生态差异[J]. 生态环境, 2006, 15(2):345-349.
[22]高山, 许端详, 林碧英, 等. 低温弱光胁迫下11 份瓠瓜种质幼苗的耐寒性[J]. 福建农业学报, 2009, 24(1):60-63.
[23]苗永美, 宁宇, 曹玉杰, 等. 黄瓜萌芽期和苗期耐冷性评价[J]. 应用生态学报, 2013, 24(7):1914-1922.
[24]阳燕娟, 来可可, 陈文明, 等. 砧用葫芦种质资源耐热性评价[J]. 南方农业学报, 2015, 46(6):1047-1052.
[25]赵杨, 魏颖娟, 邹应斌. 早稻苗期耐冷性的品种间差异及其评价[J]. 作物杂志, 2015(2):53-58.

相似文献/References:

[1]夏金婵,何奕昆.敲除DWF4基因提高拟南芥对低温胁迫的抗性[J].江苏农业学报,2015,(03):505.[doi:10.3969/j.issn.1000-4440.2015.03.007]
　XIA Jin-chan,HE Yi-kun.Improvement of cold tolerance by knockout of DWF4 gene in Arabidopsis[J].,2015,(06):505.[doi:10.3969/j.issn.1000-4440.2015.03.007]
[2]曹玉杰,薄凯亮,程春燕,等.外源尸胺增强黄瓜幼苗耐冷性的生理机制[J].江苏农业学报,2015,(01):122.[doi:10.3969/j.issn.1000-4440.2015.01.019]
　CAO Yu-jie,BO Kai-liang,CHENG Chun-yan,et al.Physiological mechanism of exogenous cadaverine enhancing the chilling tolerance of cucumber seedlings[J].,2015,(06):122.[doi:10.3969/j.issn.1000-4440.2015.01.019]
[3]杨文飞,杜永林,顾大路,等.4种调节物质对水稻耐低温能力的影响[J].江苏农业学报,2017,(04):739.[doi:doi:10.3969/j.issn.1000-4440.2017.04.003]
　YANG Wen-fei,DU Yong-lin,GU Da-lu,et al.Effects of four kinds of regulatory agents on cold-tolerance of rice[J].,2017,(06):739.[doi:doi:10.3969/j.issn.1000-4440.2017.04.003]
[4]罗璇,黄国辉,姚平,等.外源丛枝菌根真菌对低温胁迫下蓝莓幼苗抗氧化系统的影响[J].江苏农业学报,2017,(04):909.[doi:doi:10.3969/j.issn.1000-4440.2017.04.028]
　LUO Xuan,HUANG Guo-hui,YAO Ping,et al.Influence of arbuscular mycorrhizal fungi on antioxidant systems in the in vitro branch of blueberry under low temperature stress[J].,2017,(06):909.[doi:doi:10.3969/j.issn.1000-4440.2017.04.028]
[5]孙向丽,王银,谈建中,等.外源腐胺和D-精氨酸对低温胁迫下红掌生理生化指标的影响[J].江苏农业学报,2018,(01):152.[doi:doi:10.3969/j.issn.1000-4440.2018.01.022]
　SUN Xiang-li,WANG Yin,TAN Jian-zhong,et al.Effects of exogenous putrescine and D-Arg on physiological and biochemical indices of anthurium under chilling stress[J].,2018,(06):152.[doi:doi:10.3969/j.issn.1000-4440.2018.01.022]
[6]孙玉珺,秦东玲,伊凡,等.外源水杨酸对低温胁迫下玉米幼苗生长及生理特性的影响[J].江苏农业学报,2018,(04):726.[doi:doi:10.3969/j.issn.1000-4440.2018.04.002]
　SUN Yu-jun,QIN Dong-ling,YI Fan,et al.Effects of salicylic acid on growth and physiological property of maize seedling under low temperature stress[J].,2018,(06):726.[doi:doi:10.3969/j.issn.1000-4440.2018.04.002]
[7]姚悦梅,孙德举,李建斌,等.低温对羽衣甘蓝幼苗氮代谢的影响[J].江苏农业学报,2019,(05):1191.[doi:doi:10.3969/j.issn.1000-4440.2019.05.027]
　YAO Yue-mei,SUN De-ju,LI Jian-bin,et al.Effects of low temperature on nitrogen metabolism in kale seedlings[J].,2019,(06):1191.[doi:doi:10.3969/j.issn.1000-4440.2019.05.027]
[8]赵友学,杨燕波,朱宇峰,等.内生细菌Pantoea alhagi NX-11及其胞外多糖对低温胁迫下水稻苗的促生效应[J].江苏农业学报,2022,38(02):296.[doi:doi:10.3969/j.issn.1000-4440.2022.02.002]
　ZHAO You-xue,YANG Yan-bo,ZHU Yu-feng,et al.Effects of endophytic bacterium Pantoea alhagi NX-11 and its extracellular polysaccharides on the growth of paddy rice seedlings under low temperature stress[J].,2022,38(06):296.[doi:doi:10.3969/j.issn.1000-4440.2022.02.002]
[9]范军强,路晓明,王会文,等.低温胁迫下甘蓝型冬油菜抗寒性与叶片激素含量的关联性[J].江苏农业学报,2023,(01):15.[doi:doi:10.3969/j.issn.1000-4440.2023.01.002]
　FAN Jun-qiang,LU Xiao-ming,WANG Hui-wen,et al.Association between cold resistance and leaf hormone content of Brassica napus L. under low temperature stress[J].,2023,(06):15.[doi:doi:10.3969/j.issn.1000-4440.2023.01.002]
[10]刘计涛,王梦诗,索海翠,等.马铃薯GA2ox家族基因响应赤霉素（GA）和低温胁迫表达分析[J].江苏农业学报,2023,(05):1110.[doi:doi:10.3969/j.issn.1000-4440.2023.05.003]
　LIU Ji-tao,WANG Meng-shi,SUO Hai-cui,et al.Expression analysis of GA2ox family genes in response to gibberellin (GA) and cold stress in potato[J].,2023,(06):1110.[doi:doi:10.3969/j.issn.1000-4440.2023.05.003]

备注/Memo

备注/Memo:
收稿日期：2016-02-23 基金项目：国家科技支撑计划项目（2012BAD02B03-14）；国家西甜瓜产业技术体系岗位科学家项目（CARS-NO.26）；江苏省农业三新工程项目[SXGC（2015）337]；江苏省农业科技自主创新基金项目[CX（14）2004] 作者简介：张曼（1981-），女，山西运城人，博士，副研究员，研究方向为西瓜分子育种。（Tel）025-84390434；（E-mail）fldzm04@126.com 通讯作者：羊杏平，（Tel）025-84390222；（E-ma
更新日期/Last Update: 2017-02-07