[1]赵靓,白彩霞,王小朋,等.猪细小病毒6型全基因组克隆及遗传进化分析[J].江苏农业学报,2019,(05):1154-1160.[doi:doi:10.3969/j.issn.1000-4440.2019.05.022]
　ZHAO Liang,BAI Cai-xia,WANG Xiao-peng,et al.Cloning and genetic evolution analysis of whole genome of porcine parvovirus type 6[J].,2019,(05):1154-1160.[doi:doi:10.3969/j.issn.1000-4440.2019.05.022]
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猪细小病毒6型全基因组克隆及遗传进化分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年05期
页码:
1154-1160
栏目:
畜牧兽医·水产养殖
出版日期:
2019-10-31

文章信息/Info

Title:
Cloning and genetic evolution analysis of whole genome of porcine parvovirus type 6
作者:
赵靓1; 白彩霞1; 王小朋1; 杨侃侃1; 张达1; 李永东2; 孙裴1; 王勇1
(1.安徽农业大学动物科技学院，安徽合肥230036；2.宁波市疾病预防控制中心，浙江宁波315010)
Author(s):
ZHAO Liang1; BAI Cai-xia1; WANG Xiao-peng1; YANG Kan-kan1; ZHANG Da1; LI Yong-dong2; SUN Pei1; WANG Yong1
(1.Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;2.Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China)
关键词:
猪细小病毒6型; 全基因组; 同源性分析; 遗传进化分析
Keywords:
porcine parvovirus type 6; whole genome; homology analysis; genetic evolution unalysis
分类号:
S852.65
DOI:
doi:10.3969/j.issn.1000-4440.2019.05.022
文献标志码:
A
摘要:
对猪细小病毒6型(PPV6)安徽分离株PPV6 AH进行全基因组序列克隆，并进行遗传进化树构建及同源性分析。将病毒基因分10段进行PCR扩增并测序，用SeqMan进行拼接，获得全基因组序列。将PPV6 AH株与GenBank中PPV6其他毒株进行遗传进化分析。结果显示，PPV6 AH基因组全长6 180 bp，与其他国内外15株PPV6全基因的核苷酸序列同源性达到97.1%～99.5%。基于PPV6全基因、NS1及Cap基因构建的遗传进化树显示PPV6 AH株与PPV6 BJ、BJ2、SC及JS株有着共同的进化起源。NS1基因第708、996、1 932核苷酸位点发生突变；Cap基因在第1 205、1 211、1 582、1 614核苷酸位点发生突变；Cap蛋白氨基酸在第402和404位点发生突变。本研究首次报道PPV6在安徽省猪群中存在，该研究结果为安徽地区PPV6的分子遗传进化特征提供了一定的参考。
Abstract:
The whole genome of porcine parvovirus type6 (PPV6) Anhui (AH)strain was cloned, and the genetic evolution and homologies were analyzed in this study. The virus genes were divided into 10 segments for PCR amplification. After been sequenced, the SeqMan software was used for splicing to obtain the whole genome sequence. Genetic evolution analysis of PPV6 AH strain and other strains of PPV6 in GenBank was conducted. The results showed that the total length of the PPV6 AH genome was 6 180 bp. And the nucleotide homology was 97.1%-99.5% compared with other 15 domestic and foreign PPV6 genes. The genetic tree constructed based on PPV6 gene, NS1 gene and Cap gene showed that PPV6 AH strain had common evolutionary origin with PPV6 BJ, BJ2, SC and JS strain. Mutations occurred at nucleotide sites 708, 996 and 1 932 of NS1 gene. The Cap gene was mutated at nucleoside sites 1 205, 1 211, 1 582 and 1 614. Amino acids of Cap protein mutated at 402 and 404. In this study, it was first reported that PPV6 existed in pigs in Anhui province. The results of this study provided references for the molecular genetic and evolutionary characteristics of PPV6 in Anhui province.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2019-05-24 基金项目：家畜疫病病原生物学国家重点实验室开放课题(SKLVEB2016KFKT003)；安徽省自然科学基金项目(1708085MC83)；国家自然科学基金项目(31602063) 作者简介：赵靓(1995-)，女，安徽合肥人，硕士研究生，主要从事动物传染病研究。(E-mail)no.zl.jump@gmail.com；白彩霞为共同第一作者。 通讯作者：孙裴，(E-mail)sunpei@ahau.edu.cn；王勇，(E-mail)wangyong119@ahau.edu.cn
更新日期/Last Update: 2019-11-11