[1]吴旭锦,朱小甫.猪瘟病毒 ＳＸＹＬ２００６ 株全基因组序列特征分析[J].江苏农业学报,2016,(01):133-141.[doi:10.3969/j.issn.1000-4440.2016.01.021 ]
　WU Xu-jin,ZHU Xiao-fu.Complete genome sequence analysis of classical swine fever virus strain SXYL2006[J].,2016,(01):133-141.[doi:10.3969/j.issn.1000-4440.2016.01.021 ]
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猪瘟病毒 ＳＸＹＬ２００６ 株全基因组序列特征分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年01期
页码:
133-141
栏目:
畜牧兽医·水产养殖
出版日期:
2016-01-08

文章信息/Info

Title:
Complete genome sequence analysis of classical swine fever virus strain SXYL2006
作者:
吴旭锦; 朱小甫
(咸阳职业技术学院畜牧兽医研究所,动物疫病分子生物学诊断实验室,陕西咸阳712000)
Author(s):
WU Xu-jin; ZHU Xiao-fu
(Animal Epidemic Disease Diagnostic Laboratory of Molecular Biology, Institute of Animal Husbandry and Veterinary Medicine, Xianyang Vocational Technical College, Xianyang 712000, China)
关键词:
猪瘟病毒; 全基因组; 序列分析; SXYL2006
Keywords:
swine fever virus; complete genome; sequence analysis; SXYL2006
分类号:
S855.3
DOI:
10.3969/j.issn.1000-4440.2016.01.021
文献标志码:
A
摘要:
为了对分离到的猪瘟流行毒株SXYL2006进行全基因组序列克隆分析,揭示其遗传变异特征,为猪瘟分子流行病学研究提供素材,为猪瘟防控提供理论参考,根据GenBank上发表的古典猪瘟Shimen毒株及HCLV株全基因序列,参考有关文献合成了12对引物,应用RT-PCR技术,从陕西省猪瘟流行毒株SXYL2006中成功扩增出了11段cDNA,测序后拼接得到全长12 295 bp全基因序列,提交GenBank获得登录号GQ122383。将SXYL2006与参考毒株进行比较,结果表明SXYL2006与毒株39、ALD、Alfort187、Brescia、CAP、Glentorf、GPE、GXWZ02、HCLV、LPC、Paderborn、Riems和Shimen核苷酸序列同源性分别为88.2％、85.5％、85.4％、85.0％、85.1％、85.0％、85.2％、95.6％、84.4％、83.8％、94.8％、84.7％和85.1％,氨基酸序列同源性分别为94.2％、92.9％、92.9％、92.9％、92.1％、92.0％、92.5％、97.4％、91.6％、90.6％、97.5％、92.0％和92.5％。系统发生树分析结果表明SXYL2006与GXWZ02在进化上距离最近,亲缘关系最为密切,两者与德国流行毒株Paderborn同处于基因Ⅱ群。与HCLV株相比,SXYL2006株E0蛋白中,具有Rnase活性的2个主要氨基酸序列没有发生任何变异;在E2蛋白中,与免疫逃逸相关的5个位点有3个发生变异;已发现的26个T细胞表位中,有14个表位27个氨基酸位点变异。SXYL2006在遗传特性和抗原性上与经典强毒株Shimen、疫苗毒株HCLV差异较大。
Abstract:
To clone and analyze the complete genome sequence of classical swine fever virus strain SXYL2006 and reveal the genetic variation and molecular epidemiology of classical swine fever, eleven cDNA segments were amplified from SXYL2006 by RT-PCR with 12 pairs of primers synthesized according to the genome sequences of classical swine fever strains Shimen and HCLV published in GenBank. After sequencing and splicing, a full-length 12 295-bp genome sequence was obtained (GenBank accession number GQ122383). Compared with reference strains 39, ALD, Alfort187, Brescia, CAP, Glentorf, GPE, GXWZ02, HCLV, LPC, Parderborn, Riems and Shimen, the nucleotide of SXYL 2006 shared homologies of 88.2％, 85.5％, 85.4％, 85.0％, 85.1％, 85.0％, 85.2％, 95.6％, 84.4％, 83.8％, 94.8％, 84.7％ and 85.1％,respectively, and amino acid sequence shared 94.2％, 92.9％, 92.9％, 92.9％, 92.1％, 92.0％, 92.5％, 97.4％, 91.6％, 90.6％, 97.5％, 92.0％ and 92.5％ homologies, respectively. Phylogenetic tree analysis showed that SXYL2006 and GXWZ02 were the closest evolutionarily, and the two strains and German virulent strain Paderborn were classified into gene group Ⅱ, compared with HCLV strains. There was no variation in the two major amino acid sequences with Rnase activity in protein E0 encoded by SXYL2006. Three out of five sites related to immune escape in protein E2 mutated. There were 27 amino acid mutated sites in 14 out of 26 T cell epitopes. SXYL2006 differed from strains Shimen and HCLV in genetic characteristics and antigenicity.

参考文献/References:

[1]李文良,毛立,杨蕾蕾,等. 猪瘟病毒糖基化E2蛋白和E0蛋白的协同免疫保护作用[J]. 江苏农业学报, 2015, 31(2): 357-361.
[2]MEYERS G, RUMENAPF T, THIEL H J. Molecular cloning and nucleotide sequence of the genome of hog cholera virus[J].Virology,1989,171:555-567.
[3]赵永前,左建新,孙华伟,等. 某规模猪场猪瘟抗体水平的监测与分析[J]. 江苏农业科学,2015,43(11): 299-300.
[4]MOORMANN R J M, WARMERDAM P A M, MEER B V D, et al. Molecular cloning and nucleotide sequence of hog cholera virus strain Brescia and mapping of the genome region encoding envelope protein E1[J].Virology,1990,177:184-198.
[5]覃军,梁珠民,李军成. 规模化猪场主要疫病抗体监测与免疫效果分析[J]. 江苏农业科学,2015,43(10): 264-266.
[6]RUMENAPF T, UNGER G, STRAUSS J H, et al. Processing of the envelope glycoprotein of pestivirus[J]. Journal of Virology,1993,67:3288-3294.
[7]RUGGLI N, TRATSCHIN J, MITTELHOLZER C, et al. Nucleotide sequence of classical swine fever virus strain Alfort187 and transcription of infectious RNA from stably cloned full-length cDNA[J]. J Virol,1996,6:3478-3487.
[8]ISHIKAWA K, NAGAI H, KATAYAMA K, et al. Comparison of the entire nucleotide and deduced aminoacids equences of the attenuated hog cholera vaccine strain GPE and the wild type parental strain ALD[J]. Archvirol,1995,140:1385-1391.
[9]MOORMANN R J M, VAN GEN H G P, MIEDEMA G K W, et al. Infectious RNA transcribed from on engineered full-length cDNA template of the genome of apestivirus[J]. J Virol,1996,2:736-770.
[10]吴旭锦,朱小甫,朱辉. 1株山东猪瘟流行毒株全基因组克隆与序列分析[J].西北农林科技大学学报:自然科学版,2009,37(11):7-12.
[11]ELISENDA A, KARL-HEINZ W, DANIEL W, et al. Identification of T-cell epitopes in the structural and non-structural proteins of classical swine fever virus[J]. Journal of General Virology, 2002, 83: 551-560.
[12]赵永前,孙华伟,何孔旺,等. 后备母猪入群前的抗体筛查在某规模化猪场的应用[J]. 江苏农业科学,2015,43(12): 249-250.
[13]涂长春.猪瘟国际流行态势、我国现状及防制对策[J].中国农业科学，2003, 36(8): 955-960.
[14]刘金凤,吴健敏,覃绍敏,等. 抗猪瘟病毒单链抗体基因的构建及其推导的蛋白质三级结构的分子模拟[J]. 江苏农业学报,2015,31(4): 817-823.
[15]KADEN V, LANGE E, STEYER H. Does multiple oral vaccination of wild boar against classical swine fever (CSF) have a positive influence on the immunity?[J]. Deutsche Tierarztliche Wochenschrift, 2004,111(2):63-67.
[16]仇华吉,童光志,沈荣显. 猪瘟兔化弱毒疫苗--半个世纪的回顾[J].中国农业科学,2005,38(8):1675-1685.
[17]王琴,王在时,赵耘,等. 12株猪瘟病毒E2基因主要抗原区域的序列差异分析[J]. 微生物学报,2000,40(6):614-621.
[18]吴显实,罗廷荣,廖素环,等.广西猪瘟流行毒株全基因组的克隆与序列分析[J].中国兽医学报,2005,25(2):125-127.
[19]刘湘涛,韩雪清,刘伯华,等. 猪瘟流行野毒Erns基因的序列分析[J].中国农业科学,2000, 33(4): 75-81.
[20]赵耘,王在时,王琴,等. 22株猪瘟病毒E2基因部分编码序列的序列分析[J]. 微生物学通报,2001,28(5):42-48.
[21]HULST M M,PANOTO F E, HOEKMAN A, et al. Inactivation of the Rnase activity of glycoprotein Erns of classical swinefever virus in a cytopathogenic virus[J]. J Virol,1998,72(1):151-157.
[22]MEYERS G, SAALMULLER A, BUTTNER M. Mutations abrogating the Rnase activity in glycoprotein Erns of the pestivirus classical swine fever virus lead to virus attenuation[J]. J Virol, 1999, 73(12):10224-10235.
[23]IQBAL M, FLICK-SMITH H, MCCAULEY J W. Interaction of bovine viral diarrheoa virus glycoprotein Ernswith cell surface glycosaminoglycans[J]. J Gen Virol,2000,81:451-459.
[24]王琴,宁宜宝,王在时, 等. 猪瘟病毒流行株与疫苗株Erns基因的序列分析[J]. 中国农业科学，2004, 37 (3): 446-452.
[25]VAN RIJIN P A. Antigen structure of envelope glycoprotein E1 of hog cholera virus [J]. Journal of General Virology, 1994, 68:3934-3942.

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备注/Memo

备注/Memo:
收稿日期：2015-06-11 基金项目：陕西省科学技术研究发展计划项目(2014K02-06-03);咸阳市科学技术研究计划项目(2014K02-21) 作者简介：吴旭锦(1979-),女,陕西西安人,博士,副教授,主要从事动物疫病分子病原学研究工作。(Tel)029-33680108; (E-mail)yaoyuanwxj@163.com
更新日期/Last Update: 2017-05-08