[1]曹媛媛,陈春,郭婷婷,等.亲和性促生菌DW12-L的定殖及其对大豆生长的影响[J].江苏农业学报,2019,(04):776-783.[doi:doi:10.3969/j.issn.1000-4440.2019.04.004]
　CAO Yuan yuan,CHEN Chun,GUO Ting ting,et al.Colonization of soybean affinity rhizobacteria strain DW12-L and its effects on soybean growth[J].,2019,(04):776-783.[doi:doi:10.3969/j.issn.1000-4440.2019.04.004]
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亲和性促生菌DW12-L的定殖及其对大豆生长的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年04期
页码:
776-783
栏目:
遗传育种·生理生化
出版日期:
2019-08-31

文章信息/Info

Title:
Colonization of soybean affinity rhizobacteria strain DW12-L and its effects on soybean growth
作者:
曹媛媛1; 陈春1; 郭婷婷1; 钱叶1; 张程飞1; 何相怡1; 李婷1; Lay Khien Duc1; 唐欣昀1 ; 邵文韬2; 王晓波2; 邱丽娟3
（1.安徽农业大学生命科学学院，安徽合肥230036；2.安徽农业大学农学院，安徽合肥230036；3.中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程中心，北京100081）
Author(s):
CAO Yuanyuan1; CHEN Chun1; GUO Tingting1; QIAN Ye1; ZHANG Chengfei1; HE Xiangyi1; LI Ting1; LAY Khienduc1; TANG Xinyun1; SHAO Wentao2; WANG Xiaobo2; QIU Lijuan3
(1.School of Life Science, Anhui Agricultural University, Hefei 230036, China; 2. School of Agronomy, Anhui Agricultural University, Hefei 230036, China; 3.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China)
关键词:
大豆; 植物根际促生菌; 凝集素; 定殖; 促生长; 根系发育
Keywords:
soybean;  plant growth promoting rhizobacteria;  lectin;  colonization;  growth promotion;  root development
分类号:
S565.101
DOI:
doi:10.3969/j.issn.1000-4440.2019.04.004
文献标志码:
A
摘要:
为了研究大豆亲和性根际促生菌（PGPR）菌株在大豆根际的定殖及其对大豆生长发育的影响，采用电转化法将luxAB基因转入对大豆凝集素（SBA）具有亲和性的PGPR菌株DW12中。将标记菌株DW12L接种于大豆品种中黄606根际，以SBA非亲和的PGPR菌株P34L作对照，考察DW12L在大豆根际定殖的时空特征，检测菌株的定殖对大豆生长以及根系发育的影响。结果表明，DW12L可长期定居于大豆根际，并随根系的生长在新的根段根际吸附定殖，而P34L在大豆根际不能稳定存活；DW12L显著促进大豆地上部和地下部生物量的积累以及根系的发育，P34L在大豆生长30 d时由于不能定殖于根际而无显著促生效果。与大豆凝集素具有亲和性的PGPR菌株DW12L可定殖于大豆根际并促进大豆生长，定殖能力的强弱是决定菌株能否发挥促生功能的关键。
Abstract:
In order to study the colonization of plant growth promoting rhizobacteria (PGPR) in soybean rhizosphere and its effects on soybean growth and development, luxAB gene was transferred into a PGPR strain DW12 with with specific affinity to soybean agglutinin (SBA) affinity by electrotransformation. The labeled strain DW12L was inoculated in soybean (Glycine max Zhonghuang 606) rhizosphere. Using a PGPR strain P34L without affinity to SBA as control, the spatial and temproal characteristics of colonization of DW12L in soybean rhizophere were studied, and the effects of colonization on soybean (Glycine max Zhonghuang 606) growth and root development were detected. The results showed that DW12L could survive in soybean rhizosphere for a long time and grow in the new space with extension of soybean root, while P34L couldnt survive stably in soybean rhizosphere. The strain DW12L significantly promoted the accumulation of aboveground and underground biomass and root development in soybean (Glycine max Zhonghuang 606), while strain P34L had no significant effect on the growth of soybean because it could not colonize the rhizosphere after soybean grew for 30 days. The PGPR strain DW12L, which has affinity to SBA, can colonize soybean rhizosphere and promote soybean growth, and the colonization ability is an important basis for determining whether a strain can promote the soybean growth.

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备注/Memo

备注/Memo:
收稿日期：2019-03-28 基金项目：国家重点研发计划项目（2016YFD0100201）；国家自然科学基金项目(41401269、31771819)；安徽省教育厅高校自然科学研究项目（KJ2019A0183）；安徽省大学生创新创业训练计划项目(201710364106) 作者简介：曹媛媛(1980-)，女，安徽合肥人，博士，副教授，主要从事微生物与植物互作研究。(E-mail) yy721@hotmail.com；陈春为共同第一作者 通讯作者：王晓波，(E-mail)wxbphd@163.com；邱丽娟，(E-mail)qiulijuan@caas.cn
更新日期/Last Update: 2019-08-31