[1]王培培,韩榕.利用不依赖连接反应的克隆方法构建pCHF3-MAP65-1-YFP和pCHF3-MAP65-2-YFP表达载体[J].江苏农业学报,2018,(02):266-270.[doi:doi:10.3969/j.issn.1000-4440.2018.02.005]
 WANG Pei-pei,HAN Rong.Construction of the pCHF3-MAP65-1-YFP and the pCHF3-MAP65-2-YFP vectors with ligation-independent cloning method[J].,2018,(02):266-270.[doi:doi:10.3969/j.issn.1000-4440.2018.02.005]
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利用不依赖连接反应的克隆方法构建pCHF3-MAP65-1-YFP和pCHF3-MAP65-2-YFP表达载体()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年02期
页码:
266-270
栏目:
遗传育种·生理生化
出版日期:
2018-04-25

文章信息/Info

Title:
Construction of the pCHF3-MAP65-1-YFP and the pCHF3-MAP65-2-YFP vectors with ligation-independent cloning method
作者:
王培培1韩榕2
(1.山西师范大学生命科学学院,山西临汾041000;2.植物分子与环境胁迫响应山西省高等学校重点实验室,山西临汾041000)
Author(s):
WANG Pei-pei1HAN Rong2
(1.College of Life Science, Shanxi Normal University, Linfen 041000, China;2.Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response,Linfen 041000, China)
关键词:
LIC方法拟南芥MAP65-1MAP65-2
Keywords:
ligation-independent cloning(LIC) methodArabidopsis thalianaMAP65-1 MAP65-2
分类号:
S188
DOI:
doi:10.3969/j.issn.1000-4440.2018.02.005
文献标志码:
A
摘要:
利用不依赖于连接反应的克隆(Ligation-independent cloning, LIC)方法构建了pCHF3-MAP65-1-YFP和pCHF3-MAP65-2-YFP载体,用于研究拟南芥微管结合蛋白MAP65-1和MAP65-2在细胞中的定位及UV-B辐射下有丝分裂异常现象的产生机制。试验结果表明,相对于传统载体构建方法,LIC方法试验设计操作简便,耗时短,转化率高,可减少后期重组子筛选工作。
Abstract:
The method of ligation-independent cloning (LIC) was used to construct pCHF3-MAP65-1-YFP and pCHF3-MAP65-2-YFP vector that would be used for subsequent research of localization of the Arabidopsis thaliana microtubule binding protein MAP65-1 and MAP65-2 in the cell and the production mechanism of mitotic anomaly in UV-B radiation. The results showed that the method of LIC had simple operation, short time consuming, high conversion rate, and less workload of later screening recombinant, compared with the traditional method of building the carrier.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2017-12-26 基金项目:国家自然科学基金项目(30671061);山西省自然科学基金项目(2008011059-1) 作者简介:王培培(1992-),女,山西长治人,硕士研究生,研究方向为植物细胞学,(E-mail )wangpeipei6512@163.com 通讯作者:韩榕,(E-mail )hhwrsl@163.com
更新日期/Last Update: 2018-05-04