[1]王津,韩榕.DNA甲基转移酶赋予拟南芥盐胁迫耐受性[J].江苏农业学报,2019,(05):1028-1031.[doi:doi:10.3969/j.issn.1000-4440.2019.05.004]
　WANG Jin,HAN Rong.DNA methyltransferases confer salt stress tolerance in Arabidopsis thaliana[J].,2019,(05):1028-1031.[doi:doi:10.3969/j.issn.1000-4440.2019.05.004]
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DNA甲基转移酶赋予拟南芥盐胁迫耐受性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年05期
页码:
1028-1031
栏目:
遗传育种·生理生化
出版日期:
2019-10-31

文章信息/Info

Title:
DNA methyltransferases confer salt stress tolerance in Arabidopsis thaliana
作者:
王津1; 韩榕2
(1.山西师范大学生命科学学院，山西临汾041004；2.植物分子与环境胁迫响应山西省高等学校重点实验室，山西临汾041004)
Author(s):
WANG Jin1; HAN Rong2
(1.College of Life Science, Shanxi Normal University, Linfen 041004, China; 2. Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response, Linfen 041004, China)
关键词:
DNA甲基转移酶; 盐胁迫耐受性; 拟南芥
Keywords:
DNA methyltransferase;  salt stress tolerance;  Arabidopsis thaliana
分类号:
S311
DOI:
doi:10.3969/j.issn.1000-4440.2019.05.004
文献标志码:
A
摘要:
为了探究DNA甲基化修饰在植物响应盐胁迫过程中的作用，对DNA甲基转移酶的突变体在盐胁迫下的表型进行观察，发现drm1drm2cmt3三重突变体相较于野生型对盐胁迫更加敏感，而drm1drm2双重突变体和cmt3单突变体相较于野生型对盐胁迫的应答没有明显差异。定量PCR结果表明纤维素合成酶ATCSLA1和ATCSLA10在野生型中受盐胁迫诱导表达，而这种诱导表达在三重突变体中明显减弱。以上结果表明，DNA甲基转移酶可能间接促进纤维素合成酶的表达进而调控纤维素合成的水平，最终赋予拟南芥盐胁迫的耐受性。
Abstract:
In order to explore the function of DNA methylation in the process of plant response to salt stress, the phenotype of DNA methyltransferase mutants was observed under salt stress. It was found that drm1drm2cmt3 triple mutant was more sensitive to salt stress when compared with wild-type, while the drm1drm2 double mutant and cmt3 single mutant showed no significant difference. Quantitative PCR results showed that cellulose synthase ATCSLA1 and ATCSLA10 were induced by salt stress in wild-type, but the induced expression was weakened visibly in the triple mutant. The above results indicate that DNA methyltransferase may indirectly up-regulate the expression of cellulose synthase, thus regulate the level of cellulose synthesis, and finally confer plant salt stress tolerance.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2018-12-12 基金项目：国家自然科学基金项目(30671061) ; 山西省自然科学基金项目(2014011028-5) 作者简介：王津(1989-)，女，河北衡水人，硕士研究生，研究方向为植物生理学。(E-mail) jinwang2008@126.com 通讯作者：韩榕，(E-mail) hhwrsl@163.com
更新日期/Last Update: 2019-11-11