[1]高营,林云,袁星星,等.蚕豆VfGASA1基因的异源过表达延迟拟南芥开花[J].江苏农业学报,2021,(01):44-52.[doi:doi:10.3969/j.issn.1000-4440.2021.01.006]
 GAO Ying,LIN Yun,YUAN Xing-xing,et al.Heterologous overexpression of Vicia faba VfGASA1 gene delays flowering in transgenic Arabidopsis[J].,2021,(01):44-52.[doi:doi:10.3969/j.issn.1000-4440.2021.01.006]
点击复制

蚕豆VfGASA1基因的异源过表达延迟拟南芥开花()
分享到:

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年01期
页码:
44-52
栏目:
遗传育种·生理生化
出版日期:
2021-02-28

文章信息/Info

Title:
Heterologous overexpression of Vicia faba VfGASA1 gene delays flowering in transgenic Arabidopsis
作者:
高营12林云2袁星星2薛晨晨2陈新2朱月林1
(1.南京农业大学园艺学院,江苏南京210095;2.江苏省农业科学院经济作物研究所,江苏南京210014)
Author(s):
GAO Ying12 LIN Yun2 YUAN Xing-xing2 XUE Chen-chen2 CHEN Xin2 ZHU Yue-lin1
(1.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;2.Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
蚕豆VfGASA1拟南芥开花时间赤霉素
Keywords:
Vicia fabaVfGASA1Arabidopsisflowering timegibberellin
分类号:
S643.6
DOI:
doi:10.3969/j.issn.1000-4440.2021.01.006
文献标志码:
A
摘要:
GASA(Gibberellic acid-stimulated in Arabidopsis)蛋白是一类受赤霉素调控的小分子蛋白质,参与植物生长发育的多条途径。本研究在蚕豆(Vicia faba)中同源克隆了VfGASA1基因,该基因的开放阅读框全长为363 bp,编码1个包含120个氨基酸残基的蛋白质,VfGASA1在蚕豆叶片和嫩荚中高表达,亚细胞定位结果显示,VfGASA1是一个定位于质外体的分泌蛋白。异源过表达VfGASA1导致转基因拟南芥开花延迟、莲座叶数量增多,外施赤霉素能够恢复这一现象。实时荧光定量结果显示,转基因拟南芥植株中FT基因显著下调,而DELLA基因中的GAI基因显著上调,说明VfGASA1可能是通过间接抑制DELLA蛋白的表达从而调控植物开花。本研究结果为蚕豆的开花调控育种提供了理论依据。
Abstract:
Gibberellic acid-stimulated in Arabidopsis (GASA) protein is a kind of protein with small molecular weight regulated by gibberellin and participates in various pathways of plant growth and development. In this study, the GASA gene VfGASA1 in faba bean (Vicia faba) was got by homologous cloning. The open reading frame(ORF) of VfGASA1 was 363 bp in length and encoded a protein containing 120 amino acid residues. VfGASA1 was highly expressed in the leaves and capsules of faba beans, and subcellular localization showed that VfGASA1 was a secretory protein located in the apoplast. Heterologous overexpression of VfGASA1 in Arabidopsis could delay flowering and increase the number of rosette leaves, while the phenomenon could be recovered by the application of exogenous gibberellin. Results of real-time PCR (RT-PCR) showed that the expressional level of ‘florigen’ gene (FT) was significantly down-regulated in transgenic Arabidopsis while GAI (one of DELLA genes) was significantly up-regulated. Therefore, VfGASA1 might regulate the flowering of plants by indirect inhibiting the expression of DELLA genes. This study provides theoretic basis for breeding programs targeted on the flowering control of faba bean.

参考文献/References:

[1]SONG Y H, SHIM J S, KINMONTH-SCHULTZ H A, et al. Photoperiodic flowering: time measurement mechanisms in leaves[J]. Annual Review of Plant Biology, 2015, 66: 441-464.
[2]TURCK F, FORNARA F, COUPLAND G. Regulation and identity of florigen: FLOWERING LOCUS T moves center stage[J]. Annual Review of Plant Biology, 2008, 59: 573-594.
[3]KING R W, EVANS L T. Gibberellins and flowering of grasses and cereals: prizing open the lid of the “florigen” black box[J]. Annual Review of Plant Biology, 2003. 54(1): 307-328.
[4]KIM D H, DOYLE M R, SUNG S, et al. Vernalization: winter and the timing of flowering in plants[J]. Annual Review of Cell and Developmental Biology, 2009, 25(1): 277-299.
[5]PIN P A, NILSSON O. The multifaceted roles of FLOWERING LOCUS T in plant development[J]. Plant Cell and Environment, 2012, 35(10): 1742-1755.
[6]LEE J, LEE I. Regulation and function of SOC1, a flowering pathway integrator[J]. Journal of Experimental Botany, 2010, 61(9): 2247-2254.
[7]MOYROUD E, KUSTERS E, MONNIAUX M, et al. LEAFY blossoms[J]. Trends in Plant Science, 2010, 15(6): 346-352.
[8]WELLMER F, RIECHMANN J L. Gene networks controlling the initiation of flower development[J]. Trends in Genetics, 2010, 26(12): 519-527.
[9]SAMACH A, ONOUCH H, GOLD S E, et al. Distinct roles of CONSTANS target genes in reproductive development of Arabidopsis[J]. Science, 2000, 288(5471): 1613-1616.
[10]TIWARI S B, SHEN Y, CHANG H C, et al. The flowering time regulator CONSTANS is recruited to the FLOWERING LOCUS T promoter via a unique cis-element[J]. New Phytologist, 2010, 187(1): 57-66.
[11]DAVIERE J M, ACHARD P. Gibberellin signaling in plants[J]. Development, 2013, 140(6): 1147-1151.
[12]LIU Z, BOACHON B, LUGAN R, et al. A conserved cytochrome P450 evolved in seed plants regulates flower maturation[J]. Molecular Plant, 2015, 8(12): 1751-1765.
[13]OGAWA M, HANADA A, YAMAUCHI Y, et al. Gibberellin biosynthesis and response during Arabidopsis seed germination[J]. The Plant Cell, 2003, 15(7): 1591-1604.
[14]SRIKANTH A, MARKUS S. Regulation of flowering time: all roads lead to Rome[J]. Cellular and Molecular Life Sciences, 2011, 68(12): 2013-2037.
[15]WANG Y, ZHAO J, LU W, et al. Gibberellin in plant height control: old player, new story[J]. Plant Cell Reports, 2017, 36(3): 391-398.
[16]MURASE K, HIRANO Y, SUN T, et al. Gibberellin-induced DELLA recognition by the gibberellin receptor GID1[J]. Nature, 2008, 456(7221): 459-463.
[17]GALVAO V C, HORRER D, KUTTNER F, et al. Spatial control of flowering by DELLA proteins in Arabidopsis thaliana[J]. Development, 2012, 139(21): 4072-4082.
[18]XU F, LI T, XU P B, et al. DELLA proteins physically interact with CONSTANS to regulate flowering under long days in Arabidopsis[J]. FEBS Letters, 2016, 590(4): 541-549.
[19]Li M, AN F, LI W, et al. DELLA proteins interact with FLC to repress flowering transition[J]. Journal of Integrative Plant Biology, 2016, 58(7): 642-655.
[20]GRIFFITHS J, MURASE K, RIEU I, et al. Genetic characterization and functional analysis of the GID1 gibberellin receptors in Arabidopsis[J]. The Plant Cell Online, 2006, 18(12): 3399-3414.
[21]WILLIGE B C, GHOSH S, NILL C, et al. The DELLA domain of GA INSENSITIVE mediates the interaction with the GA INSENSITIVE DWARF1A gibberellin receptor of Arabidopsis[J]. The Plant Cell Online, 2007, 19(4): 1209-1220.
[22]SHI L, GAST R T, GOPALRAJ M, et al. Characterization of a shoot-specific, GA3- and ABA-regulated gene from tomato[J]. The Plant Journal: for Cell and Molecular Biology, 1992, 2(2): 153-159.
[23]LEE S C, KIM S, HAN H K, et al. A gibberellin-stimulated transcript, OsGASR1, controls seedling growth and alpha-amylase expression in rice[J]. Journal of Plant Physiology, 2017, 214: 116-122.
[24]ZIMMERMANN R, SAKAI H, HOCHHOLDINGER F. The Gibberellic Acid Stimulated-Like gene family in maize and its role in lateral root development[J]. Plant Physiology, 2010, 152(1): 356-365.
[25]SUN S, WANG H, YU H, et al. GASA14 regulates leaf expansion and abiotic stress resistance by modulating reactive oxygen species accumulation[J]. Journal of Experimental Botany, 2013, 64(6): 1637-1647.
[26]DE LA FUENTE JOSE I, IRAIDA A, CRISTINA C, et al. The strawberry gene FaGAST affects plant growth through inhibition of cell elongation[J]. Journal of Experimental Botany, 2006, 57(10): 2401-2412.
[27]ZHANG S, WANG X. One new kind of phytohormonal signaling integrator: up-and-coming GASA family genes[J]. Plant Signaling & Behavior, 2017, 12(2): 6453-6459.
[28]NAHIRNAK V, NATALIA INES A, HORACIO ESTEBAN H, et al. Snakin/GASA proteins: involvement in hormone crosstalk and redox homeostasis[J]. Plant Signaling & Behavior, 2012, 7(8): 1004-1008.
[29]AUBERT D, CHEVILLARD M C, DORNE A M, et al. Expression patterns of GASA genes in Arabidopsis thaliana: the GASA4 gene is up-regulated by gibberellins in meristematic regions[J]. Plant Molecular Biology, 1998, 36(6): 871-883.
[30]ZHANG S C, WANG X J. Expression pattern of GASA, downstream genes of DELLA, in Arabidopsis[J]. Chinese Science Bulletin, 2008, 53(24): 3839-3846.
[31]ROXRUD I, LID S E, FLETCHER J C, et al. GASA4, one of the 14-member Arabidopsis GASA family of small polypeptides, regulates flowering and seed development[J]. Plant & Cell Physiology, 2007, 48(3): 471-483.
[32]ZHANG S, YANG C, PENG J, et al. GASA5, a regulator of flowering time and stem growth in Arabidopsis thaliana[J]. Plant Molecular Biology, 2009, 69(6): 745-759.
[33]O’SULLIVAN D M , DEEPTI A. Advances in faba bean genetics and genomics[J]. Frontiers in Genetics, 2016(7): 150-160.
[34]GAO B, BIAN X C, YANG F, et al. Comprehensive transcriptome analysis of faba bean in response to vernalization[J]. Planta, 2019, 251(1): 22-41.
[35]CAO Y Y, BIAN X C, CHEN M X, et al. iTRAQ-based quantitative proteomic analysis in vernalization-treated faba bean (Vicia faba L.)[J]. PLoS One, 2017, 12(11): 10-28.
[36]COOPER J W, WILSON M H, DERKS M F L, et al. Enhancing faba bean (Vicia faba L.) genome resources[J]. Journal of Experimental Botany, 2017, 68(8): 1941-1953.
[37]CRUZ-IZQUIERDO S, AVILA C M, SATOVIC Z, et al. Comparative genomics to bridge Vicia faba with model and closely-related legume species: stability of QTLs for flowering and yield-related traits[J]. Theoretical and Applied Genetics, 2012, 125(8): 1767-1782.
[38]YANG K, TIAN Z, CHEN C, et al. Genome sequencing of adzuki bean (Vigna angularis) provides insight into high starch and low fat accumulation and domestication[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(43): 13213-13218.
[39]LIU Q, DONNER E, YIN Y, et al. The physicochemical properties and in vitro digestibility of selected cereals, tubers and legumes grown in China[J]. Food Chemistry, 2006, 99(3): 470-477.
[40]NAYAK G K, ROBERTS S P M, GARRATT M, et al. Interactive effect of floral abundance and semi-natural habitats on pollinators in field beans (Vicia faba)[J]. Agriculture Ecosystems & Environment, 2015, 199(27): 58-66.
[41]BISHOP J, JONES H E, O’SULLIVAN D M, et al. Elevated temperature drives a shift from selfing to outcrossing in the insect-pollinated legume, faba bean (Vicia faba) [J]. Journal of Experimental Botany, 2017, 68(8): 2055-2063.
[42]KAUR S, KIMBER R B E, COGAN N O I, et al. SNP discovery and high-density genetic mapping in faba bean (Vicia faba L.) permits identification of QTLs for ascochyta blight resistance[J]. Plant Science, 2014, 217/218(41): 47-55.
[43]CRUZ-LZQUIERDO S, AVILA C M, SATOVIC Z, et al. Comparative genomics to bridge Vicia faba with model and closely-related legume species: stability of QTLs for flowering and yield-related traits[J]. Theoretical and Applied Genetics, 2012, 125(8): 1767-1782.

相似文献/References:

[1]欧阳裕元,余东梅,杨梅.蚕豆主要农艺性状与单株产量的相关及通径分析[J].江苏农业学报,2016,(04):763.[doi:10.3969/j.issn.100-4440.2016.04.008]
 OUYANG Yu-yuan,YU Dong-mei,YANG Mei.Path analysis and correlation analysis between agronomic traits and yield in broad bean[J].,2016,(01):763.[doi:10.3969/j.issn.100-4440.2016.04.008]
[2]刘飞,杨春艳,谢建新.傅里叶变换红外光谱结合判别分析法诊断蚕豆病虫害[J].江苏农业学报,2015,(03):531.[doi:10.3969/j.issn.1000-4440.2015.03.011]
 LIU Fei,YANG Chun-yan,XIE Jian-xin.Diagnosis of diseases and pests of broad bean by Fourier transform infrared spectroscopy combining discriminant analysis[J].,2015,(01):531.[doi:10.3969/j.issn.1000-4440.2015.03.011]
[3]陈惠,唐明霞,宋居易,等.烫漂对蚕豆感官品质及过氧化物酶活性的影响[J].江苏农业学报,2015,(03):708.[doi:10.3969/j.issn.1000-4440.2015.03.038]
 CHEN hui,TANG Ming-xia,SONG Ju-yi,et al.Effect of blanching on sensory properties and activity of peroxidase in broad beans[J].,2015,(01):708.[doi:10.3969/j.issn.1000-4440.2015.03.038]
[4]涂丽琴,吴淑华,干射香,等.江苏省蚕豆上菜豆黄花叶病毒的分子鉴定[J].江苏农业学报,2019,(04):804.[doi:doi:10.3969/j.issn.1000-4440.2019.04.008]
 TU Li qin,WU Shu hua,GAN She xiang,et al.Molecular identification of bean yellow mosaic virus infecting Vicia faba from Jiangsu province[J].,2019,(01):804.[doi:doi:10.3969/j.issn.1000-4440.2019.04.008]
[5]周仙莉,滕长才,张红岩,等.蚕豆遗传图谱与QTL定位研究进展[J].江苏农业学报,2021,(01):237.[doi:doi:10.3969/j.issn.1000-4440.2021.01.031]
 ZHOU Xian-li,TENG Chang-cai,ZHANG Hong-yan,et al.Research advance of genetic linkage map and QTL location in Vicia faba L.[J].,2021,(01):237.[doi:doi:10.3969/j.issn.1000-4440.2021.01.031]
[6]辛佳佳,张南峰,程华萍,等.江西省地方蚕豆种质资源遗传多样性分析及优异资源挖掘[J].江苏农业学报,2022,38(01):20.[doi:doi:10.3969/j.issn.1000-4440.2022.01.003]
 XIN Jia-jia,ZHANG Nan-feng,CHENG Hua-ping,et al.Genetic diversity analysis and excellent resources mining of local broad bean germplasm resources in Jiangxi province[J].,2022,38(01):20.[doi:doi:10.3969/j.issn.1000-4440.2022.01.003]
[7]赵娜,缪亚梅,姚梦楠,等.蚕豆种质资源籽粒表型与营养品质性状的多样性分析[J].江苏农业学报,2022,38(03):597.[doi:doi:10.3969/j.issn.1000-4440.2022.03.003]
 ZHAO Na,MIAO Ya-mei,YAO Meng-nan,et al.Diversity analysis on seed phenotypic and nutrient quality traits in faba bean germplasm resources[J].,2022,38(01):597.[doi:doi:10.3969/j.issn.1000-4440.2022.03.003]
[8]高晓晓,涂丽琴,孙枫,等.江苏蚕豆三叶草黄脉病毒的分子鉴定及全基因组结构特征分析[J].江苏农业学报,2022,38(05):1203.[doi:doi:10.3969/j.issn.1000-4440.2022.05.006]
 GAO Xiao-xiao,TU Li-qin,SUN Feng,et al.Molecular identification and genomic characterization of clover yellow vein virus isolated from broad bean in Jiangsu province[J].,2022,38(01):1203.[doi:doi:10.3969/j.issn.1000-4440.2022.05.006]
[9]周恩强,周瑶,姚梦楠,等.基于全长转录组的蚕豆WRKY基因家族分析及耐盐胁迫相关候选基因挖掘[J].江苏农业学报,2024,(01):14.[doi:doi:10.3969/j.issn.1000-4440.2024.01.002]
 ZHOU En-qiang,ZHOU Yao,YAO Meng-nan,et al.Analysis of WRKY gene family based on full-length transcriptome and mining of salt stress candidate genes in Vicia faba[J].,2024,(01):14.[doi:doi:10.3969/j.issn.1000-4440.2024.01.002]

备注/Memo

备注/Memo:
收稿日期:2020-04-29基金项目:国家食用豆产业技术体系生物防治与综合防控岗位科学家项目(CARS-08-G15);江苏特粮特经产业技术体系集成创新中心项目[JATS(2019)399]作者简介:高营(1994-),女,河南南阳人,硕士研究生,从事豆类作物分子育种研究。(E-mail)3099618289@qq.com通讯作者:朱月林,(E-mail)ylzhu@njau.edu.cn
更新日期/Last Update: 2021-03-15