[1]覃悦,祝友朋,韩长志.基于全基因组序列的黄单胞菌分泌蛋白质预测及其特征分析[J].江苏农业学报,2021,(01):53-59.[doi:doi:10.3969/j.issn.1000-4440.2021.01.007]
　QIN Yue,ZHU You-peng,HAN Chang-zhi.Prediction and characteristic analysis of Xanthomonas campestris secretory protein based on whole genome sequence[J].,2021,(01):53-59.[doi:doi:10.3969/j.issn.1000-4440.2021.01.007]
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基于全基因组序列的黄单胞菌分泌蛋白质预测及其特征分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年01期
页码:
53-59
栏目:
植物保护
出版日期:
2021-02-28

文章信息/Info

Title:
Prediction and characteristic analysis of Xanthomonas campestris secretory protein based on whole genome sequence
作者:
覃悦1; 祝友朋1; 韩长志1; 2
（1.西南林业大学生物多样性保护学院，云南昆明650224；2.云南省森林灾害预警与控制重点实验室，云南昆明650224）
Author(s):
QIN Yue1; ZHU You-peng1; HAN Chang-zhi1; 2
(1.College of Biodiversity Conservation and Utilization, Southwest Forestry University, Kunming 650224,China;2.Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Kunming 650224, China)
关键词:
黄单胞菌; 全基因组; 信号肽; 生物信息学
Keywords:
Xanthomonas campestris; genome; signal peptide; bioinformatics
分类号:
S435.1
DOI:
doi:10.3969/j.issn.1000-4440.2021.01.007
文献标志码:
A
摘要:
黄单胞菌（Xanthomonas campestris）作为诸多革兰氏阴性细菌之一，主要危害十字花科农作物和核桃、杧果等经济林植物。前人对植物病原细菌、真菌等的分泌蛋白和相关蛋白质开展了预测及分析研究，明确其分泌蛋白在致病过程中具有非常重要的作用。本研究以已经公布全基因组序列的黄单胞菌X.campestris B100、X. campestris pv.campestris str.8004、X. campestris CN14中的蛋白质序列为基础，结合细菌中分泌蛋白的基本特征，采用在线分析程序（包括SignalP v5.0、ProtComp v9.0等）对上述3个黄单胞菌的分泌蛋白序列进行预测和分析，明确上述细菌中分别含有135个、128个、135个分泌蛋白，并对分泌蛋白所具有的基本特征开展分析，为深入研究黄单胞菌分泌蛋白的功能打下理论基础。
Abstract:
As a kind of many Gram-negative bacteria, Xanthomonas campestris mainly harms cruciferous crops and economic forest plants such as walnut and mango. Some academic predecessors discovered that secretory proteins played an important role in the pathogenic process by predicting and analyzing the secreted proteins and related proteins of plant pathogenic bacteria and fungi. This study was based on the protein sequences of X. campestris B100, X. campestris pv. campestris str.8004 and X. campestris CN14 whose whole genome sequences had been published, combined with the basic characteristics of secretory proteins, using online analysis programs (such as SignalP v5.0, ProtComp v9.0, etc.) to predict and analyze the above three protein sequences, it was found that the bacteria contained 135, 128 and 135 secretory proteins, respectively, and analysis on the basic characteristics of secretory proteins was carried out, thus made theoretical foundation for the in-depth study of the function of X. campestris secretory proteins.

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备注/Memo

备注/Memo:
收稿日期：2020-04-28基金项目：云南省应用基础研究计划项目（2018FG001-028）；国家自然科学基金项目（31960314）；西南林业大学大学生创新创业项目（201825）作者简介：覃悦（1992-），云南昆明人，硕士研究生，研究方向为经济林木病害生物防治与真菌分子生物学。（E-mail）453276403@qq.com通讯作者：韩长志，（E-mail）hanchangzhi2010@163.com
更新日期/Last Update: 2021-03-15