[1]夏雯雯,李锦,祝建波.天山雪莲水孔蛋白基因(AQP)家族鉴定与生物信息学分析[J].江苏农业学报,2016,(06):1244-1250.[doi:doi:10.3969/j.issn.1000-4440.2016.06.008]
　XIA Wen-wen,LI Jin,ZHU Jian-bo.Identification and bioinformatics analysis of aquaporin gene (AQP) family in Saussurea involucrate Kar.[J].,2016,(06):1244-1250.[doi:doi:10.3969/j.issn.1000-4440.2016.06.008]
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天山雪莲水孔蛋白基因(AQP)家族鉴定与生物信息学分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年06期
页码:
1244-1250
栏目:
遗传育种·生理生化
出版日期:
2017-02-07

文章信息/Info

Title:
Identification and bioinformatics analysis of aquaporin gene (AQP) family in Saussurea involucrate Kar.
作者:
夏雯雯; 李锦; 祝建波
（石河子大学生命科学学院农业生物技术重点实验室，新疆石河子832000）
Author(s):
XIA Wen-wen; LI Jin; ZHU Jian-bo
(Laboratory of Agricultural Biotechnology, College of Life Science, Shihezi University, Shihezi 832000, China)
关键词:
天山雪莲; 拟南芥; 水孔蛋白基因家族; 生物信息学
Keywords:
Saussurea involucrate Kar.; Arabidopsis thaliana; AQP gene family; bioinformatics
分类号:
Q811.4
DOI:
doi:10.3969/j.issn.1000-4440.2016.06.008
文献标志码:
A
摘要:
为研究新疆天山雪莲水孔蛋白基因（AQP）家族并对其基因家族成员进行鉴定及分类，从雪莲转录组数据库中获得了33条天山雪莲水孔蛋白基因序列，利用生物信息学软件，将其与拟南芥中38条水孔蛋白基因序列进行分析、分类和保守序列分析，结果显示天山雪莲33条AQP基因家族序列与拟南芥38条AQP基因序列一样分成4个亚族，即质膜水孔蛋白（Plasma membrane intrinsic protein，PIP）基因、液泡膜内在水孔蛋白（Tonoplast intrinsic protein, TIP）基因、类Nodulin（NOD26）膜内在水孔蛋白（NOD26-like membrane intrinsic protein, NIP）基因和膜内小基本蛋白（Small and basic membrane intrinsic protein, SIP）基因。拟南芥与天山雪莲AQP基因间具有较高的保守性。对天山雪莲AQP基因家族进行了系统进化树构建，并分析了水孔蛋白氨基酸组成成分、理化性质，预测了二级结构，发现水孔蛋白不同亚族间氨基酸数目、氨基酸序列间的亲水性存在一定差异，水孔蛋白的二级结构以α螺旋、β折叠、无规则卷曲为主。从这33条AQP基因序列中克隆了3个水孔蛋白基因，测序结果证明天山雪莲转录组数据库基因序列准确率较高。
Abstract:
To identify the aquaporin (AQP) gene family of Saussurea involucrate Kar., 33 sequences of the AQP were acquired from transcriptome database. Using a variety of bioinformatics softwares, the gene sequences of S. involucrate Kar. AQP and Arabidopsis thaliana AQP were analyzed and classified. 33 S. involucrate Kar. AQP genes as well as 38 Arabidopsis AQP genes were classified into four subfamilies, plasma membrane intrinsic protein gene(PIP), tonoplast intrinsic protein gene（TIP）, NOD26-like membrane intrinsic protein gene(NIP) and small and basic membrane intrinsic protein gene(SIP). AQP genes were highly conservative between Arabidopsis and S. involucrate Kar.. There were some differences in the number of amino acids and the hydrophilicity of amino acid sequence among different subfamilies of AQP gene. The secondary structure of AQP protein were dominated by alpha helix, beta bridge, and random coil. The sequencing of three AQP gene cloned from 33 AQP gene sequences verified the accuracy of gene sequences in transcriptome database.

参考文献/References:

[1]AGRE P. Aquaporin water channels (Nobel lecture) [J]. Angewandte Chemie International Edition, 2004,43(33): 4278-4290.

[2]〖JP+1〗BENGA G. Birth of water channel proteins——the aquaporins[J]. Cell Biology International， 2003,27(9): 701-709.
[3]PRESTON G M, CARROLL T P, GUGGINO W B, et al. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein[J]. Science, 1992,256(5055): 385-387.
[4]JOHANSON U, KARLSSON M, JOHANSSON I, et al. The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants[J]. Plant Physiology, 2001,126(4): 1358-1369.
[5]JOHANSON U, GUSTAVSSON S. A new subfamily of major intrinsic proteins in plants[J]. Mol Biol Evol, 2002,19(4): 456-461.
[6]AROCA R, PORCEL R, RUIZ-LOZANO J M. Regulation of root water uptake under abiotic stress conditions[J]. J Exp Bot, 2012,63(1): 43-57.
[7]SAHARA T, GODA T, OHGIYA S. Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature[J]. J Biol Chem, 2002, 277:50015-50021.
[8]TANGHE A. Aquaporin expression correlates with freeze tolerance in baker’s yeast, and overexpression improves freeze tolerance in industrial strains[J]. Appl Environ Microbiol, 2002, 68:5981-5989.
[9]TOURNAIRE-ROUX C, SUTKA M, JAVOT H, et al. Cytosolic pH regulates root water transport during anoxic stress through gating of aquaporins[J]. Nature, 2003, 425（6956）: 393-397.
[10]UEHLEIN N, LOVISOLO C, SIEFRITZ F, et al. The tobacco aquaporin NtAQP1 is a membrane CO2 pore with physiological functions[J]. Nature, 2003, 425 (6959): 734-737.
[11]GE Y X, ANGENENTG C, DAHLHAUS E, et al. Partial silencing of the NEC1 gene results in early opening of anthers in Petunia hybrida [J]. Mol Genet Genomics, 2001, 265(3): 414-423.
[12]SAITOU N, NEI M. The neighbor-joining method: a new method for reconstructing phylogenetic trees[J]. Mol Biol Evo, 1987, 4(4):406-425.
[13]TAMURA K, DUDLEY J, NEI M, et al. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0[J]. Mol Biol and Evo1, 2007, 24(8): 1596-1599.
[14]WUDICK M M, LUU D T, MAUREL C. A look inside: localization patterns and functions of intracellular plant aquaporins[J]. New Phytologist, 2009, 184(2): 289-302.
[15]PENG Y H, LI W L, CAI W M, et al. Overexpression of a Panax ginseng tonoplast aquaporin alters salt tolerance, drought tolerance and cold acclimation ability in transgenic Arabidopsis plants[J]. Planta, 2007, 226: 729-740.
[16]LI X J, WANG X H, YANG Y, et al. Single-molecule analysis of PIP2-1 dynamics and partitioning reveals multiple modes of arabidopsis plasma membrane aquaporin regulation[J]. The Plant Cell, 2011, 23: 3780-3797.
[17]BOURSIAC Y B J, POSTAIRE O, LUU D T, et al. Stimulus-induced downregulation of root water transport involves reactive oxygen species-activated cell signalling and plasma membrane intrinsic protein internalization[J]. The Plant Journal, 2008, 56(2):207-218.
[18]焦天奇，孙辉，刘瑞娜,等.转天山雪莲sikPIP3基因烟草的获得及抗逆性鉴定[J].西北植物学报，2012,32(3):431-438.

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备注/Memo

备注/Memo:
收稿日期：2015-10-22 基金项目：国家自然科学基金项目（C020406） 作者简介：夏雯雯（1988-），女，新疆乌鲁木齐人，硕士研究生，主要从事植物基因工程研究。(E-mail)365689825@qq.com 通讯作者：祝建波，(E-mail)274831213@qq.com
更新日期/Last Update: 2017-02-07