[1]杨杰,潘孝青,赵艳红,等.硒对新西兰兔皮肤抗氧化性及GPx4、EGFR 蛋白表达的影响[J].江苏农业学报,2017,(01):133-140.[doi:10.3969/j.issn.1000-4440.2017.01.021 ]
　YANG Jie,PAN Xiao-qing,ZHAO Yan-hong,et al.Effects of selenium on skin antioxidant and expression of GPx4 and EGFR proteins in New Zealand rabbit[J].,2017,(01):133-140.[doi:10.3969/j.issn.1000-4440.2017.01.021 ]
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硒对新西兰兔皮肤抗氧化性及GPx4、EGFR 蛋白表达的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年01期
页码:
133-140
栏目:
畜牧兽医·水产养殖
出版日期:
2017-02-28

文章信息/Info

Title:
Effects of selenium on skin antioxidant and expression of GPx4 and EGFR proteins in New Zealand rabbit
作者:
杨杰1; 潘孝青1; 赵艳红2; 宋丹丹3; 秦枫1; 邵乐1; 李健1; 张霞1; 张琳琳3; 王菁3; 潘翠玲3
（1.江苏省农业科学院畜牧研究所，江苏南京210014；2.江苏省农业科学院国家兽用生物制品工程技术研究中心，江苏南京210014；3.南京农业大学动物医学院，江苏南京210014)
Author(s):
YANG Jie1; PAN Xiao-qing1; ZHAO Yan-hong2; SONG Dan-dan3; QIN Feng1; SHAO Le1; LI Jian1; ZHANG Xia1; ZHANG Lin-lin3; WANG Jing3; PAN Cui-ling3
(1.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.National Research Center of Veterinary Biologicals Engineering and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;3.Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls， Nanjing Agricultural University, Nanjing 210014, China）
关键词:
硒; 新西兰兔; 抗氧化性; GPx4蛋白; 表皮生长因子受体（EGFR）
Keywords:
selenium; New Zealand rabbit; the antioxidant capacity; GPx4; EGFR
分类号:
S816.72
DOI:
10.3969/j.issn.1000-4440.2017.01.021
文献标志码:
A
摘要:
为了探讨硒对新西兰兔毛囊发育的影响机制，本研究将60只初始体质量为 （690.0±7.0） g的新西兰兔分为C和SS 2组，每组30只（雌雄各半）。C组直接饲喂基础日粮，SS组日粮添加亚硒酸钠，经测定日粮硒含量分别为0.20 mg/kg和0.36 mg/kg，饲养30 d。结果表明，C组新西兰兔的毛囊群结构排列整齐，1个初级毛囊周围环绕数个次级毛囊，且毛囊横切面呈正常椭圆形。SS组新西兰兔的毛囊群结构呈无序排列，初级毛囊和部分次级毛囊的横切面不是正常的椭圆形。SS组次级毛囊数量比C组低36.64%（P<0.05）。在皮肤抗氧化性方面，与C组相比，SS组皮肤组织中谷胱甘肽过氧化物酶4(GPx4)活性、超氧化物歧化酶(SOD)活性、总抗氧化物(T-AOC)含量分别降低58.09%（P<0.01）、34.39%（P<0.05）和59.25%（P<0.01），丙二醛（MDA）含量升高242.66%（P<0.05）。与C组相比，SS组新西兰兔皮肤毛囊中GPx4蛋白、表皮生长因子受体（EGFR）蛋白表达量分别降低131.70%（P<0.01）和128.60%（P<0.01）。
Abstract:
To investigate the effect of selenium on hair follicle development and expression of GPx4 protein in rabbit hair, 60 New Zealand rabbits which initial weight was （690.0±7.0） g were divided into two groups(C and SS), 30 rabbits in each group (male and female). The C group was fed on basal diet, the determination of dietary selenium level was 0.20 mg/kg, SS group of dietary sodium selenite, the determination of dietary selenium level was 0.36 mg/kg. Feeding 30 d. Rabbit hair follicle group structure of C group arranged neatly, a primary hair follicle was surrounded by a number of secondary follicles, hair follicles and oval cross section was normal. New Zealand rabbit hair follicle group structure of SS group was disordered, transverse section of primary follicles and part of secondary follicles were not normal. The number of secondary follicles in SS group was decreased by 36.64% than that in C group （P<0.05）. The antioxidant properties of the skin, compared with the C group, the GPx4 activity of SS group in skin tissue significantly decreased by 58.09% （P<0.01）, SOD activity significantly decreased by 34.39% （P<0.05）, T-AOC activity significantly decreased by 59.25% （P<0.01）, the content of MDA significantly increased by 242.66% （P<0.05）.The expression of GPx4 and EGFR proteins of New Zealand rabbit skin hair follicles in SS group were significantly decreased by the 131.70%（P<0.01）and 128.60% （P<0.01） than that in C group.

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备注/Memo

备注/Memo:
收稿日期：2016-09-01 基金项目：江苏省农业自主创新基金项目[CX（14）2078]  作者简介：杨杰(1972-),男,安徽蒙城人,硕士,副研究员,主要从事家兔饲料营养调控研究。（E-mail）jieyangbwx@hotmail.com
更新日期/Last Update: 2017-04-12