[1]李隐侠,张俊,钱勇,等.湖羊NR5A1 基因SNPs 筛选及其与产羔数的关联分析[J].江苏农业学报,2017,(01):124-132.[doi:10.3969/j.issn.1000-4440.2017.01.020 ]
　LI Yin-xia,ZHANG Jun,QIAN Yong,et al.SNPs selection of NR5A1 and its association analysis with litter size in Hu sheep[J].,2017,(01):124-132.[doi:10.3969/j.issn.1000-4440.2017.01.020 ]
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湖羊NR5A1 基因SNPs 筛选及其与产羔数的关联分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年01期
页码:
124-132
栏目:
畜牧兽医·水产养殖
出版日期:
2017-02-28

文章信息/Info

Title:
SNPs selection of NR5A1 and its association analysis with litter size in Hu sheep
作者:
李隐侠12; 张俊12; 钱勇12; 孟春花12; 王慧利12; 钟声12; 曹少先12
（1.江苏省农业科学院畜牧研究所，江苏南京210014;2.江苏省农业科学院动物品种改良和繁育重点实验室，江苏南京210014）
Author(s):
LI Yin-xia12; ZHANG Jun12; QIAN Yong12; MENG Chun-hua12; WANG Hui-li12; ZHONG Sheng12; CAO Shao-xian12
（1.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China；2.Key Laboratory of Animal Breeding and Reproduction, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China）
关键词:
湖羊; NR5A1基因; SNPs; PCR-RFLP; AS-PCR; 关联分析
Keywords:
Hu sheep; NR5A1; SNPs; PCR-RFLP; AS-PCR; correlation analysis
分类号:
S826
DOI:
10.3969/j.issn.1000-4440.2017.01.020
文献标志码:
A
摘要:
为探讨NR5A1基因能否作为湖羊产羔性状的候选基因，同时寻找与湖羊繁殖性能相关的分子标记，以高繁殖力湖羊为研究对象，采用DNA池结合测序方法筛选NR5A1基因在湖羊中的SNPs位点，并利用PCR-RFLP和AS-PCR方法进行多态位点分型，利用SPSS软件分析不同基因型与湖羊产羔数的相关性。结果表明，在湖羊NR5A1基因中检测到6个SNPs位点,分析发现g.6052A/G位点为TaqI酶切位点，其余5个位点处没有酶切位点存在。在湖羊群体中，g.3362G/C（GG、GC、CC）、g.4342G/A(GG、GA、AA)、 g.4666C/T(CC、CT、TT) 、g.6052A/G(GG、AG、AA) 和g.6991T/G(TT、TG、GG)位点均检测到3种基因型，而g.5699C/G位点在湖羊群体中仅发现2种基因型GG和GC。6个位点的多态信息含量（PIC）从0.306到0.375不等，均属于中度多态（0.25
Abstract:
In order to explore whether NR5A1 gene can be used as a candidate gene of the litter size of Hu sheep，SNPs were screened and the correlation between different genotypes and litter size of Hu sheep were analyzed. High fecundity sheep were used as the research objective, the SNPs of NR5A1 gene was screened using DNA pools combining with sequencing, and the correlations between different genotypes and litter size of Hu sheep were analyzed using SPSS software. Six SNPs locus were detected in Hu sheep population. g.6052A/G locus owned the TaqI enzyme site and other five SNPs were absent enzyme sites. In Hu sheep population, there were three genotypes in the loci of g.3362G/C（GG, GC, CC）, g.4342G/A(GG, GA, AA), g.4666C/T(CC, CT, TT), g.6052A/G(GG, AG, AA) and g.6991T/G(TT, TG, GG) and only two genotypes in g.5699C/G (GG, GC) locus. The PIC of these six SNPs ranged from 0.306 to 0.375 and belonged to moderate polymorphism (0.25

参考文献/References:

[1]IKEDA Y, LALA D S, LUO X，et al. Characterization of the mouse FTZ-F1 gene, which encodes a key regulator of steroid hydroxylase gene expression [J]. Molecular Endocrinology, 1993, 7(7):852-860.
[2]MYNARCZUK J, REKAWIECKI R. The role of the orphan receptor SF-1 in the development and function of the ovary [J]. Reprod Biol，2010, 10(3): 177-193.
[3]KIM K W, LI S, ZHAO H, et al. CNS-specific ablation of steroidogenic factor 1 results in impaired female reproductive function. [J]. Mol Endocrinol,2010, 24(6): 1240-1250.
[4]YIN M, LYU M, YAO G, et al. Transactivation of micro RNA-383 by steroidogenic factor-1 promotes estradiol release from mouse ovarian granulosa cells by targeting RBMS1[J]. Mol Endocrinol, 2012, 26(7): 1129-1143. 
[5]FALENDER A E, LANZ R, MALENFANT D, et al. Differential expression of steroidogenic factor-1 and FTF/LRH-1 in the rodent ovary [J]. Endocrinology,2003, 144(8): 3598-3610. 
[6]SADOVSKY Y, DORN C. Function of steroidogenic factor 1 during development and differentiation of the reproductive system [J]. Reviews of Reproduction, 2000, 5(3):136-142.
[7]LUO X, IKEDA Y, PARKER K L. A cell-specific nuclear receptor is essential for adrenal and gonadal development and sexual differentiation [J]. Cell , 1994, 77(4):481-490.
[8]JEYASURIA P, IKEDA Y, JAMIN S P, et al. Cell-specific knockout of steroidogenic factor 1 reveals its essential roles in gonadal function [J]. Mol Endocrinol,2004, 18(7): 1610-1619. 
[9]BUAAS F W, GARDINER J R, CLAYTON S, et al. In vivo evidence for the crucial role of SF1 in steroid-producing cells of the testis, ovary and adrenal gland [J]. Development, 2012, 139(24): 4561-4570. 
[10]LIN L, PHILIBERT P, FERRAZ-DE-SOUZA B, et al. Heterozygoous missense mutations in steroidogenic factor 1(SF1/Ad4BP, NR5A1) are associated with 46, XY disorders of sex development with normal adrenal function [J]. J Clin Endocrinol Metab, 2007, 92(3):991-999.
[11]ACHERMANN J C, ITO M, HINDMARSH P C, et al. A mutation in the gene encoding steroidogenic factor-1 causes XY sex reversal and adrenal failure in humans [J]. Nature Genetics, 1999, 22(2):125-126.
[12]LAKHAL B, BEN-HADJ-KHALIFA S, BOUALI N,et al. Mutational screening of SF1 and WNT4 in Tunisian women with premature ovarian failure [J]. Gene,2012, 509(2): 298-301. 
[13]FERLIN A, ROCCA M S, VINANZI C, et al.Mutational screening of NR5A1 gene encoding steroidogenic factor 1 in cryptorchidism and male factor infertility and functional analysis of seven undescribed mutations [J].Fertil Steril,2015,104(1):163-169.
[14]KAWABE S, YAZAWA T, KANNO M,et al.A novel isoform of liver receptor homolog-1 is regulated by steroidogenic factor-1 and the specificity protein family in ovarian granulosa cells [J].Endocrinology,2013, 54(4):1648-1660. 
[15]KARPOVA T, RAVICHANDIRAN K, INSISIENMAY L, et al. Steroidogenic factor 1 differentially regulates fetal and adult leydig cell development in male mice [J]. Biol Reprod,2015, 93(4):83.
[16]MLYNARCZUK J, WROBEL M H, REKAWIECKI R, et al.The expression of steroidogenic Factor-1 and its role in bovine steroidogenic ovarian cells during the estrus cycle and first trimester of pregnancy [J]. Anim Reprod Sci,2013,138(1/2):74-81.
[17]SMITH T P, SHOWALTER A D, SLOOP K W, et al.Identification of porcine Lhx3 and SF1 as candidate genes for QTL affecting growth and reproduction traits in swine [J].Anim Genet,2001, 32(6):344-350.
[18]MUSILLE P M, PATHAK M, LAUER J L, et al. Divergent sequence tunes ligand sensitivity in phospholipid-regulated hormone receptors [J]. J Biol Chem,2013, 288(28): 20702-20712. 
[19]LABELLE-DUMAIS C, PAR J F, BLANGER L, et al. Impaired progesterone production in Nr5a2+/- mice leads to a reduction in female reproductive function [J]. Biology of Reproduction, 2007, 77(2): 217-225.
[20]IMAMICHI Y, MIZUTANI T, JU Y,et al. Transcriptional regulation of human ferredoxin 1 in ovarian granulosa cells [J]. Mol Cell Endocrinol,2013, 370(1/2): 1-10. 
[21]高爱琴，李宁，李金泉，等. 山羊FGF5基因单核甘酸多态性群体遗传学分析[J]. 华北农学报，2006，21（3）：71-76.
[22]PHILIBERT P, PARIS F, LAKHAL B, et al. NR5A1 (SF-1) gene variants in a group of 26 young women with XX primary ovarian insufficiency [J]. Fertil Steril,2013, 99(2): 484-489.

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备注/Memo

备注/Memo:
收稿日期：2016-05-18 基金项目：江苏省农业科技自主创新基金项目[CX（14）5031]；江苏省自然科学基金项目（BK20140750）；国家自然科学基金项目（31501934） 作者简介：李隐侠(1979-)，女，河南固始人，博士，副研究员，主要从事动物遗传育种与繁殖方面的研究。（Email）liyxmh@126.com 通讯作者：曹少先， （Email）sxcao@jaas.ac.cn
更新日期/Last Update: 2017-04-12