[1]宋志忠,许建兰,张斌斌,等.叶面喷施钾肥对霞脆桃果实品质及KUP基因表达的影响[J].江苏农业学报,2018,(05):1107-1112.[doi:doi:10.3969/j.issn.1000-4440.2018.05.020]
　SONG Zhi-zhong,XU Jian-lan,ZHANG Bin-bin,et al.Effect of foliar spraying of potassium fertilizer on Xiacui peach quality and expression of KUP transporter family genes[J].,2018,(05):1107-1112.[doi:doi:10.3969/j.issn.1000-4440.2018.05.020]
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叶面喷施钾肥对霞脆桃果实品质及KUP基因表达的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年05期
页码:
1107-1112
栏目:
园艺
出版日期:
2018-10-25

文章信息/Info

Title:
Effect of foliar spraying of potassium fertilizer on Xiacui peach quality and expression of KUP transporter family genes
作者:
宋志忠; 许建兰; 张斌斌; 俞明亮; 马瑞娟
（江苏省农业科学院果树研究所，江苏省高效园艺作物遗传改良重点实验室，江苏南京210014）
Author(s):
SONG Zhi-zhong;  XU Jian-lan;  ZHANG Bin-bin;  YU Ming-liang;  MA Rui-juan
(Institute of Pomology, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China)
关键词:
桃; 果实发育; 钾肥; 叶面喷施; KT/HAK/KUP家族; 基因表达
Keywords:
peach;  fruit development; potassium fertilizer; foliar spraying; KT/HAK/KUP family; gene expression
分类号:
S662.1
DOI:
doi:10.3969/j.issn.1000-4440.2018.05.020
文献标志码:
A
摘要:
以霞脆桃为材料，研究了叶面喷施钾肥对果实品质的影响及果实发育中K+动态平衡与KUP家族基因表达水平之间的联系。结果表明：喷施钾肥显著增加了霞脆桃的鲜质量和体积大小，改善了果皮着色指标，提高了果实可溶性固形物和可溶性糖含量，有效提升了桃果实品质，并有效增强了果实发育不同时期果肉的钾素营养状况；桃KT/HAK/KUP家族基因在桃果实发育不同时期的表达水平存在差异，PpeKUP1基因在霞脆桃整个果实发育期的表达水平最高，其次是PpeKUP5和PpeKUP3，而PpeKUP14～PpeKUP16在果实发育不同时期均没有检测到表达量；PpeKUP1、PpeKUP2和PpeKUP7在转录水平上对喷施钾肥处理最为敏感，其表达水平与对照相比，在第2次果实膨大期（75 DAFB）至达到商业成熟度时（95 DAFB）均显著增强。
Abstract:
The effects of potassium (K+) fertilizer spraying on Xiacui peach fruit quality and K+ homeostasis status in relation to KUP gene expression were analyzed during whole fruit development process. Results showed that K+ fertilizer spraying significantly enhanced Xiacui peach fruit quality, as evidenced by increased fruit weight and size, improved fruit skin color parameters, strengthened soluble solid content and soluble sugar content. Fertilizer spraying also enhanced the fruit K+ nutritional status. In addition, KT/HAK/KUP family genes were distinctly expressed during different fruit development stages. PpeKUP1 was the most expressed gene, followed by PpeKUP5 and PpeKUP3, whereas PpeKUP14-PpeKUP16 were not detected throughout whole fruit development process. Moreover, expression of PpeKUP1, PpeKUP2 and PpeKUP7 were more sensitive to K+ fertilizer spraying, the expression levels in the treatment of fertilizer spraying significantly increased compared with those in the control, especially from the second growth phase (75 DAFB) to commercial harvest phase (95 DAFB).

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备注/Memo

备注/Memo:
收稿日期：2018-01-30 基金项目：国家自然科学基金项目（31501743）；江苏省农业科技自主创新基金项目[CX(15)1020]；国家现代农业产业技术体系建设专项基金项目（CARS-30）;江苏省政府留学奖学金资助项目（JS-2016-190） 作者简介：宋志忠（1983-），男，山东菏泽人，博士，副研究员，主要进行果树分子生物学研究。(Tel)025-84390855;(E-mail)szhzh2000@163.com 通讯作者：马瑞娟，(Tel)025-84390220;(E-mail)marj311@163.com
更新日期/Last Update: 2018-11-05