[1]刘金洋,林云,陈景斌,等.绿豆C3H和NBS转录因子家族成员鉴定及盐胁迫响应分析[J].江苏农业学报,2023,(05):1097-1109.[doi:doi:10.3969/j.issn.1000-4440.2023.05.002]
　LIU Jin-yang,LIN Yun,CHEN Jing-bin,et al.Identification and salt stress response analysis of mungbean C3H and NBS transcription factor family members[J].,2023,(05):1097-1109.[doi:doi:10.3969/j.issn.1000-4440.2023.05.002]
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绿豆C3H和NBS转录因子家族成员鉴定及盐胁迫响应分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年05期
页码:
1097-1109
栏目:
遗传育种·生理生化
出版日期:
2023-08-31

文章信息/Info

Title:
Identification and salt stress response analysis of mungbean C3H and NBS transcription factor family members
作者:
刘金洋; 林云; 陈景斌; 闫强; 薛晨晨; 吴然然; 陈新; 袁星星
（江苏省农业科学院经济作物研究所/江苏省高效园艺作物遗传改良重点实验室，江苏南京210014）
Author(s):
LIU Jin-yang; LIN Yun; CHEN Jing-bin; YAN Qiang; XUE Chen-chen; WU Ran-ran; CHEN Xin; YUAN Xing-xing
(Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory of Efficient Horticultural Crop Genetic Improvement, Nanjing 210014, China)
关键词:
绿豆; NBS基因家族; C3H基因家族; 盐胁迫; VrNBS20转录因子
Keywords:
mungbean; NBS gene family; C3H gene family; salt stress; VrNBS20 transcription factor
分类号:
Q786
DOI:
doi:10.3969/j.issn.1000-4440.2023.05.002
文献标志码:
A
摘要:
NBS和C3H是植物体内2个重要的转录因子家族，在调控植物抗病与耐盐方面不可或缺。本研究通过转录组数据分析、qRT-PCR分析，分别鉴定出30个和289个绿豆C3H和NBS家族成员，2个基因家族各有13个基因受到纯化选择，并且C3H和NBS基因家族种内共线性关系均为片段重复。耐盐材料的转录组数据分析结果表明，VrC3H5、VrC3H7、VrC3H10和VrC3H13等4个基因的表达量在盐胁迫后发生显著改变。VrC3H5，VrC3H7和VrC3H13 3个基因对脱落酸（ABA）处理、氯化钠（NaCl）处理、干旱胁迫都有不同程度的响应，VrC3H5在ABA处理后基因表达量上调超过了10倍。在NBS基因中，有85个基因在盐胁迫10 d和15 d后出现显著差异表达，其中9个NBS基因表达变化值|log2 FC|(FC为表达倍数变化)大于2。VrNBS20转录因子通过调控EVM0022385参与绿豆的耐盐功能，VrNBS20可能是绿豆抗病与耐盐调控网络中的交叉点。本研究结果为绿豆耐盐与抗病研究提供了丰富的基因资源。
Abstract:
NBS and C3H are two important transcription factor families in plants, which are indispensable in regulating plant disease resistance and salt tolerance. In this study, through transcriptome data analysis and qRT-PCR analysis, 30 and 289 mungbean C3H and NBS family members were identified, respectively. Thirteen C3H and 13 NBS genes were purified and selected, and the intraspecific collinearity analysis of C3H and NBS gene families was fragment duplication. The transcriptome data analysis of salt-tolerant materials showed that the expression levels of VrC3H5, VrC3H7, VrC3H10 and VrC3H13 were significantly changed after salt stress. VrC3H5, VrC3H7 and VrC3H13 had different degrees of response to abscisic acid (ABA) treatment, sodium chloride (NaCl) treatment and drought stress. The expression of VrC3H5 was up-regulated by more than 10 times after ABA treatment. A total of 85 NBS genes were significantly differentially expressed after 10 days and 15 days of salt stress, of which nine NBS genes had a change in expression value |log2 FC| (FC was the expression fold change) greater than two. VrNBS20 was involved in the salt tolerance function of mung bean by regulating EVM0022385, and VrNBS20 may be the intersection point in the disease resistance and salt tolerance regulatory network of mungbean. The results of this study provide abundant genetic resources for the study of salt tolerance and disease resistance of mungbean.

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备注/Memo

备注/Memo:
收稿日期：2022-11-29 基金项目：科学技术部重点研发政府间国际合作项目（2019YFE0109100）；江苏省一带一路国际合作项目（BZ2022005）；国家食用豆产业技术体系项目（CARS-08-G15）；江苏省种业揭榜挂帅项目[JBGS（2021）004]；国家自然科学基金项目(32200499)；江苏省林业科技创新与推广项目[LYKJ（2021）22] 作者简介：刘金洋（1988-），男，安徽亳州人，博士，助理研究员，主要从事豆类作物分子育种研究。（E-mail）20200024@jaas.ac.cn 通讯作者：陈新，（E-mail）cx@jaas.ac.cn;袁星星，（E-mail）yxx@jaas.ac.cn
更新日期/Last Update: 2023-09-13