ZHOU Xu-xu,LIU Jin-yang,CHEN Xin,et al.Identification of Alfin1-like gene family in Vigna radiata (L.) Wilczek and its expression analysis under drought stress[J].,2022,38(05):1179-1187.[doi:doi:10.3969/j.issn.1000-4440.2022.05.004]





Identification of Alfin1-like gene family in Vigna radiata (L.) Wilczek and its expression analysis under drought stress
ZHOU Xu-xu12LIU Jin-yang2CHEN Xin2XUE Chen-chen2CHEN Jing-bin2LIN Yun2YAN Qiang2WU Ran-ran2ZHU Yue-lin1YUAN Xing-xing2
(1.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;2.Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory of Efficient Horticultural Crop Genetic Improvement, Nanjing 210014, China)
Vigna radiata (L.) WilczekAlfin1-like gene familydrought stress
Alfin1-like(AL)转录因子家族在对非生物胁迫的反应中具有重要调控作用。为了探究绿豆[Vigna radiata (L.)Wilczek] AL基因家族成员在干旱胁迫下的表达情况,本研究在前期以苏绿1号为试验材料完成全基因组测序和盐胁迫下转录组测序的基础上,采用同源比对的方法鉴定绿豆AL转录因子(VrAL)家族基因,分析其生物学特性;用1/2浓度Hoagland营养液培养绿豆幼苗,对照组(CK)无聚乙二醇(PEG 6000)、处理组含有20% PEG 6000,采用qRT-PCR方法探索VrAL基因家族成员在干旱胁迫下基因表达的差异。结果表明:(1)共鉴定到10个VrAL基因,根据其在染色体上的位置依次命名为VrAL1~VrAL10,其编码区长度范围为717~765 bp。(2)10个VrAL基因均含有4个内含子和5个外显子,其中8个VrAL基因的启动子区域存在抗逆和植物激素响应元件。(3)qRT-PCR分析结果表明,处理组根中VrAL1、VrAL5、VrAL7和VrAL9上调表达,叶中VrAL8、VrAL9和VrAL10上调表达,它们在绿豆对干旱胁迫响应中发挥了重要的作用。
Alfin1 like (AL) transcription factor family plays an important regulatory role in response to abiotic stress. To explore the expression pattern of AL gene family members of Vigna radiata (L.) Wilczek under drought stress, the VrAL transcription factor family genes of mung bean were identified by homologous alignment and their biological characteristics were analyzed, based on our previous work of whole genome sequencing and transcriptome sequencing of the mung bean material Sulv No.1 under salt stress. Mung bean seedlings were cultured with 1/2 concentration of Hoagland nutrient solution, the control (CK) did not contain polyethylene glycol 6000 (PEG6000) and the treatment group contained 20% PEG6000. The expression differences of VrAL gene family members under drought stress were detected by qRT-PCR. The results showed that, a total of ten VrAL genes were identified and named as VrAL1- VrAL10 according to their positions on the chromosomes, and the lengths of their coding regions ranged from 717 bp to 765 bp. The above ten VrAL genes all contained four introns and five exons, of which eight VrAL genes contained stress resistant and phytohormone response elements in their promoter regions. Results of qRT-PCR analysis showed that under the PEG 6000 conditions the expression levels of VrAL1, VrAL5, VrAL7 and VrAL9 in roots were up-regulated, and VrAL8, VrAL9 and VrAL10 in leaves were up-regulated. The up-regulated VrAL genes may play important roles in responses to drought stresses of mung bean.


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更新日期/Last Update: 2022-11-07