[1]陈亚辉,张师瑒,杨庆山,等.多枝柽柳叶片响应NaCl胁迫的转录组分析[J].江苏农业学报,2022,38(05):1188-1202.[doi:doi:10.3969/j.issn.1000-4440.2022.05.005]
　CHEN Ya-hui,ZHANG Shi-yang,YANG Qing-shan,et al.Transcriptome analysis of Tamarix ramosissima leaves in response to NaCl stress[J].,2022,38(05):1188-1202.[doi:doi:10.3969/j.issn.1000-4440.2022.05.005]
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多枝柽柳叶片响应NaCl胁迫的转录组分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年05期
页码:
1188-1202
栏目:
遗传育种·生理生化
出版日期:
2022-10-31

文章信息/Info

Title:
Transcriptome analysis of Tamarix ramosissima leaves in response to NaCl stress
作者:
陈亚辉1; 张师瑒2; 杨庆山3; 宋志忠4; 姜姜1
(1.南京林业大学林学院，江苏南京210037；2.不列颠哥伦比亚大学理学院，温哥华V6T 1Z4；3.山东省林业科学研究院盐碱地造林试验站，山东济南250000；4.鲁东大学农林工程研究院，山东烟台264039)
Author(s):
CHEN Ya-hui1; ZHANG Shi-yang2; YANG Qing-shan3; SONG Zhi-zhong4; JIANG Jiang1
(1.College of Forestry, Nanjing Forestry University, Nanjing 210037, China；2.Faculty of Science， University of British Columbia, Vancouver V6T 1Z4, Canada；3.Saline-alkali Afforestation Experimental Station, Shandong Academy of Forestry Sciences， Jinan 250000, China；4.The Engineering Research Institute of Agriculture and Forestry, Ludong University， Yantai 264039, China)
关键词:
多枝柽柳; NaCl胁迫; 转录组测序; 转录因子; MAPK信号传导途径
Keywords:
Tamarix ramosissima; NaCl stress; transcriptome sequencing; transcription factor; MAPK signal transduction pathway
分类号:
Q753
DOI:
doi:10.3969/j.issn.1000-4440.2022.05.005
文献标志码:
A
摘要:
在转录组层面解析盐生植物多枝柽柳响应盐胁迫的分子机制，为进一步挖掘耐盐关键基因提供参考。用200 mmol/L NaCl处理多枝柽柳0 h、48 h、168 h后，采集叶片进行转录组测序，分析差异表达基因(DEGs)并进行qRT-PCR验证。转录组测序原始数据拼接出Unigenes 105 702个，同时在KEGG、KOG、NR和SwissProt 4大功能数据库中检索到注释的Unigenes为27 670个。NaCl胁迫处理48 h后，多枝柽柳叶片中6 374个基因的表达水平上调，5 380个基因的表达水平下调；NaCl胁迫处理168 h后，叶片中有3 837个基因的表达水平上调，7 808个基因的表达水平下调。根据注释到KEGG 通路中的DEGs，筛选获得8个表达差异极其显著的DEGs，主要编码WRKY和bZIP家族转录因子。此外，由KEGG通路分析可知，MAPK信号转导途径可能参与多枝柽柳生长发育及其对NaCl胁迫的应答反应。本研究通过测定和解析在高盐胁迫下多枝柽柳叶片转录组信息，揭示了盐胁迫激活MAPK信号转导途径以及WRKY、MYB和bZIP等转录因子参与耐盐调控过程，为进一步研究多枝柽柳耐盐分子机制奠定了基础。
Abstract:
To uncover the molecular mechanism of halophyte Tamarix ramosissima (T. ramosissima) in response to salt stress was analyzed at the transcriptome level to provide references for further exploration of key salt-tolerant genes. T. ramosissima plants were treated with 200 mmol/L NaCl for 0 h, 48 h and 168 h, respectively, and the leaves were collected for transcriptome sequencing. The differentially expressed genes (DEGs) were excavated and further verified by qRT-PCR. In total, 105 702 unigenes were spliced from the raw data of transcriptome sequencing, of which 27 670 were retrieved from KEGG, KOG, NR and SwissProt. After 48 hours of NaCl stress treatment, the expression levels of 6 374 genes in T. ramosissima leaves were up-regulated and 5 380 genes were down-regulated. After 168 hours of NaCl stress treatment, the expression levels of 3 837 genes were up-regulated and 7 808 genes were down-regulated. According to the DEGs annotated to the KEGG pathway, eight highly differentially expressed DEGs were obtained, which mainly encoded transcription factors such as WRKY and bZIP families. In addition, the analysis of KEGG pathway showed that MAPK signal transduction pathway may be involved in the growth and development of T. ramosissima and its response to NaCl stress. This research article aims to measure and analyze the transcriptome information of T. ramosissima under high salinity stress conditions. This article also reveals that the salt stress activates the MAPK signal transduction pathway and WRKY, MYB, bZIP and other possible transcription factors involved in the salt tolerance regulation process. This research builds a foundation for future research on transcriptome information of T. ramosissima.

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备注/Memo

备注/Memo:
收稿日期：2022-01-20基金项目：山东省农业良种工程项目(2019LZGC009)；国家自然科学基金面上项目(32071612)；国家留学基金委项目(202108320311)作者简介：陈亚辉(1990-)，男，江苏泰州人，博士，助理研究员，研究方向为生态修复。(E-mail)chenyahui01@163.com通讯作者：姜姜， (E-mail)ecologyjiang@gmail.com
更新日期/Last Update: 2022-11-07