[1]王燕霞,吴季荣,俞明正,等.小麦根际土壤中禾谷多黏菌实时荧光定量PCR检测体系的建立及应用[J].江苏农业学报,2015,(05):962-966.[doi:doi:10.3969/j.issn.1000-4440.2015.05.003]
　WANG Yan-xia,WU Ji-rong,YU Ming-zheng,et al.Establishment and application of a real-time fluorescent quantitative PCR system for detection of Polymyxa graminis in rhizosphere soil of wheat[J].,2015,(05):962-966.[doi:doi:10.3969/j.issn.1000-4440.2015.05.003]
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小麦根际土壤中禾谷多黏菌实时荧光定量PCR检测体系的建立及应用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年05期
页码:
962-966
栏目:
遗传育种·生理生化
出版日期:
2015-10-31

文章信息/Info

Title:
Establishment and application of a real-time fluorescent quantitative PCR system for detection of Polymyxa graminis in rhizosphere soil of wheat
作者:
王燕霞123; 吴季荣23; 俞明正23; 赵晶晶23
（1.南京师范大学生命科学学院，江苏南京210046；2.江苏省农业科学院食品质量与安全检测研究所，江苏南京210014；3.江苏转基因安全评价公共技术服务中心，江苏南京210014）
Author(s):
WANG Yan-xia123; WU Ji-rong23; YU Ming-zheng23; ZHAO Jing-jing23; XING Yu-jun23; XU Jian-hong23; SHI Jian-rong23
(1.College of Life Science, Nanjing Normal University, Nanjing 210046, China;2.Institute of Food Quality Safety and Detection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;3.Center for Safety Evaluation of Transgenic Crops, Nanjing 210014, China)
关键词:
禾谷多黏菌; 实时荧光定量PCR; 转基因小麦
Keywords:
Polymyxa graminis; quantitative real-time PCR; transgenic wheat
分类号:
S512.1
DOI:
doi:10.3969/j.issn.1000-4440.2015.05.003
文献标志码:
A
摘要:
为了研究转基因小麦N12-1对其根际土壤中禾谷多黏菌（Polymyxa graminis）数量的影响，建立了基于实时荧光定量PCR（Quantitative Real-Time PCR）方法检测多黏菌数量的体系，并将该体系用于转基因小麦N12-1及其受体扬麦158不同生长期根际土壤中禾谷多黏菌数量的检测。结果显示，标准曲线的扩增效率为95%，相关系数为0.999，熔解曲线呈现单一峰，符合荧光定量扩增的各项指标;在各个生长期，转基因小麦N12-1与其受体扬麦158根际土壤中的禾谷多黏菌数量没有显著差异。由此推测，转基因小麦N12-1的种植对其根际土壤中禾谷多黏菌的数量没有显著影响。
Abstract:
A quantitative real-time PCR system for the detection of the number of Polymyxa graminis was established in this study. In the system, the standard curve showed a linear correlation between the input DNA and the CT values, with PCR efficiency of 95% and the correlation coefficient of 0.999, and the melting curve presented a single peak, indicative of the feasibility of the system. The quantity of P. graminis in the rhizosphere soil of transgenic wheat N12-1 and its parental material Yangmai158 during growth stages was detected using the system. No significant difference was detected in the quantity of P. graminis between transgenic wheat N12-1 and Yangmai158. The results suggested that the planting of transgenic wheat N12-1 had few adverse effects on the quantity of P. graminis in rhizosphere soil.

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备注/Memo

备注/Memo:
收稿日期：2015-06-16 基金项目：青海省农林科学院创新基金重大专项项目（2014-NKY-203）；青海省（应用）基础研究计划项目（2013-Z-721） 作者简介：叶景秀（1979-），女，山东临沂人，硕士，助理研究员，现主要从事蛋白质组学和小麦育种方面的研究工作。（E-mail）sdyejingxiu@163.com 通讯作者：史建荣，（Tel） 025-84392001；（E-mail）shiji@jaas.ac.cn
更新日期/Last Update: 2015-10-31