[1]唐茂翔,李斌,闫芳芳,等.促生抗旱微生物对烟草抗旱性的调控作用[J].江苏农业学报,2025,(12):2315-2325.[doi:doi:10.3969/j.issn.1000-4440.2025.11.004]
　TANG Maoxiang,LI Bin,YAN Fangfang,et al.Drought-resistance regulatory mechanism of plant growth-promoting and drought-tolerant microorganisms in tobacco under drought stress[J].,2025,(12):2315-2325.[doi:doi:10.3969/j.issn.1000-4440.2025.11.004]
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促生抗旱微生物对烟草抗旱性的调控作用()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年12期
页码:
2315-2325
栏目:
遗传育种·生理生化
出版日期:
2025-12-31

文章信息/Info

Title:
Drought-resistance regulatory mechanism of plant growth-promoting and drought-tolerant microorganisms in tobacco under drought stress
作者:
唐茂翔1; 李斌2; 闫芳芳3; 李鹏1; 李建鹏1; 韩勤1; 高永翔1; 刘东阳4; 邱忠平1
(1.西南交通大学生命科学与工程学院,四川成都610031；2.四川省烟草公司泸州市公司,四川泸州646699；3.四川省烟草公司攀枝花市公司,四川攀枝花617000；4.四川省烟草公司凉山州公司,四川凉山615000)
Author(s):
TANG Maoxiang1; LI Bin2; YAN Fangfang3; LI Peng1; LI Jianpeng1; HAN Qin1; GAO Yongxiang1; LIU Dongyang4; QIU Zhongping1
(1.School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China；2.Luzhou Branch of Sichuan Provincial Tobacco Company, Luzhou 646699, China；3.Panzhihua Branch of Sichuan Provincial Tobacco Company, Panzhihua 617000, China；4.Liangshan Branch of Sichuan Provincial Tobacco Company, Liangshan 615000, China)
关键词:
干旱胁迫; 烟草; 促生抗旱微生物; 植株抗旱性; 土壤酶活
Keywords:
drought stress; tobacco; growth-promoting and drought-resistant microorganisms; plant drought resistance; soil enzyme activity
分类号:
S572
DOI:
doi:10.3969/j.issn.1000-4440.2025.11.004
文献标志码:
A
摘要:
为缓解干旱胁迫对烟草生长的影响,本研究从干旱烟草根际土壤中筛选无拮抗作用的促生抗旱微生物,对其进行鉴定,并以无菌水为对照(CK),通过促生抗旱微生物单独及复配的灌根试验,分析其对干旱胁迫下烟草生理生化指标及土壤微生物群落结构的影响。结果表明,共鉴定得到7株具有溶磷、产吲哚乙酸(IAA)、产铁载体和产1-氨基环丙烷-1-羧酸(ACC)脱氨酶能力的菌株,其中菌株L5N1促生能力最强,菌株S2-1抗旱能力最强。L5N1菌株和S2-1菌株经鉴定分别为居中克吕沃尔氏菌(Kluyvera intermedia)和亚麻假单胞菌(Pseudomonas lini)。促生抗旱微生物可改善烟草农艺性状,降低烟叶丙二醛含量,提升烟草抗氧化能力,其中菌株L5N1和S2-1复配处理的效果最为显著。干旱胁迫21 d,L5N1和S2-1复配处理土壤含水率较CK提高63.20%,土壤蔗糖酶活性、脱氢酶活性、脲酶活性和酸性磷酸酶活性分别较CK提高457.14%、416.91%、53.54%和93.80%,假单胞菌属(Pseudomonas)、出芽菌属(Gemmata)、酸杆菌属(Acidibacter)细菌的相对丰度显著高于CK。综上,本研究筛选得到的促生菌株L5N1和抗旱菌株S2-1复配能有效提升烟草在干旱胁迫下的生长性能和适应能力。
Abstract:
In order to alleviate the effects of drought stress on the growth of tobacco, non-antagonistic growth-promoting and drought-resistant microorganisms from the rhizosphere soil of drought-stressed tobacco were screened and identified in this study, and sterile water was used as control (CK), the effects of drought-resistant microorganisms on physiological and biochemical indices of tobacco and soil microbial community structure under drought stress were analyzed by root irrigation experiments with single and compound drought-resistant microorganisms. The results showed that a total of seven strains with the ability to dissolve phosphorus, produce indoleacetic acid (IAA), produce siderophore and produce 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase were identified. Among them, strain L5N1 had the strongest growth-promoting ability, and strain S2-1 had the strongest drought resistance. L5N1 and S2-1 were identified as Kluyvera intermedia and Pseudomonas lini respectively. The growth-promoting and drought-resistant microorganisms could improve the agronomic traits of tobacco, reduce the malondialdehyde content of tobacco leaves, and improve the antioxidant capacity of tobacco. Among them, the combination of strains L5N1 and S2-1 had the most significant effect. After 21 days of drought stress, the soil moisture content of L5N1 and S2-1 combined treatment was 63.20% higher than that of CK, and the soil enzyme activities of sucrase, dehydrogenase, urease and acid phosphatase were 457.14%, 416.91%, 53.54% and 93.80% higher than those of CK, respectively. The relative abundance of Pseudomonas, Gemmata and Acidibacter was significantly higher than that of CK. In summary, the combination of the growth-promoting strain L5N1 and the drought-resistant strain S2-1 screened in this study can effectively improve the growth performance and adaptability of tobacco under drought stress.

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备注/Memo

备注/Memo:
收稿日期：2025-03-11基金项目：国家自然科学基金项目(52570179)；中国烟草总公司四川省公司科技专项(SCYC202409)；西南交通大学博士创新项目(CX2025YB17)作者简介：唐茂翔(2000-),男,四川宜宾人,硕士研究生,主要从事农业微生物研究。(E-mail)mxtang2000@163.com通讯作者：邱忠平,(E-mail)zhpqiu@home.swjtu.edu.cn；刘东阳,(E-mail)9038823@qq.com
更新日期/Last Update: 2026-01-20