[1]李慧楠,董伟清,邱祖杨,等.干旱胁迫下芋根系转录组分析[J].江苏农业学报,2025,(11):2097-2106.[doi:doi:10.3969/j.issn.1000-4440.2025.11.003]
　LI Huinan,DONG Weiqing,QIU Zuyang,et al.Transcriptome analysis of taro roots under drought stress[J].,2025,(11):2097-2106.[doi:doi:10.3969/j.issn.1000-4440.2025.11.003]
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干旱胁迫下芋根系转录组分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年11期
页码:
2097-2106
栏目:
遗传育种·生理生化
出版日期:
2025-11-30

文章信息/Info

Title:
Transcriptome analysis of taro roots under drought stress
作者:
李慧楠1; 董伟清1; 邱祖杨2; 刘莉莉2; 何芳练1
(1.广西农业科学院蔬菜研究所,广西南宁530007；2.荔浦市农业农村局,广西荔浦546600)
Author(s):
LI Huinan1; DONG Weiqing1; QIU Zuyang2; LIU Lili2; HE Fanglian1
(1.Vegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China;2.Lipu City Agriculture and Rural Affairs Bureau, Lipu 546600, China)
关键词:
芋; 干旱胁迫; 转录组测序; MAPK信号通路; 类胡萝卜素生物合成途径
Keywords:
taro;  drought stress; transcriptome sequencing; MAPK signaling pathway; carotenoid biosynthesis pathway
分类号:
S632.3
DOI:
doi:10.3969/j.issn.1000-4440.2025.11.003
文献标志码:
A
摘要:
为探究芋根系对干旱胁迫的分子响应,本研究以荔浦芋1号为材料,对干旱胁迫下的芋根系取样进行转录组测序。通过对比干旱组和对照组的基因表达差异、GO富集分析和KEGG富集分析,明确芋根系响应干旱胁迫的关键通路,并进一步筛选关键通路相关基因,分析它们在干旱胁迫下的表达模式。结果显示,干旱胁迫下共筛选到芋根系差异表达基因1 063个,其中上调表达基因473个,下调表达基因590个。GO富集分析结果表明,差异表达基因富集突出的生物过程为细胞过程和代谢过程,细胞组分富集最多的为细胞解剖实体,分子功能富集突出的为催化活性和结合过程。KEGG富集分析结果表明，差异表达基因富集最多的是次生代谢生物合成通路。统计分析促分裂原活化蛋白激酶（MAPK）信号通路、类胡萝卜素生物合成通路中差异表达基因数量,分别筛选出上调差异表达基因15个、4个,下调差异表达基因12个、1个。分析结果表明,MAPK信号通路、类胡萝卜素生物合成通路相关基因的表达受到干旱胁迫的影响,MAPK可能通过结合脱落酸(ABA)、活性氧(ROS)信号通路响应干旱胁迫,类胡萝卜素可能通过分解生成有机物,以及作为ABA前体物质的方式响应干旱胁迫。本试验结果可以为芋响应干旱胁迫分子机制研究和抗旱品种选育提供理论基础和参考依据。
Abstract:
To explore the molecular response of taro roots to drought stress, this study used Lipu Taro No.1 as the material, sampled taro roots under drought stress, and performed transcriptome sequencing. By comparing the differences in gene expression between the drought group and the control group, as well as conducting GO enrichment analysis and KEGG enrichment analysis, the key pathways of taro roots in response to drought stress were identified. Furthermore, genes related to these key pathways were identified, and their expression patterns under drought stress were analyzed. The results showed that a total of 1 063 differentially expressed genes (DEGs) were identified in taro roots under drought stress, including 473 up-regulated genes and 590 down-regulated genes. The GO enrichment analysis indicated that the prominent biological processes enriched by DEGs were cellular processes and metabolic processes. The most enriched cellular component was cellular anatomical entities, and the prominent mole-cular functions were catalytic activity and binding processes. The KEGG enrichment analysis showed that the most significantly enriched pathway for the differentially expressed genes was the biosynthesis of secondary metabolites. Through statistical analysis of the number of DEGs in the mitogen-activated protein kinase (MAPK) signaling pathway and carotenoid biosynthesis pathway, 15 and four up-regulated DEGs, and 12 and one down-regulated DEGs were identified, respectively. The analysis revealed that the expression of genes related to the MAPK signaling pathway and carotenoid biosynthesis pathway was affected by drought stress. MAPK may respond to drought stress by interacting with abscisic acid (ABA) and reactive oxygen species (ROS) signaling pathways, while carotenoids may respond to drought stress by decomposing to produce organic substances and serving as a precursor of ABA. The results of this study lay a theoretical foundation for research on the molecular mechanisms of drought response in taro and provide a valuable resource for breeding drought-resistant cultivars.

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备注/Memo

备注/Memo:
收稿日期：2024-12-19基金项目：国家自然科学基金项目(32460756)；广西农业科学院基本科研业务专项(桂农科2024YP081、桂农科2025YP082)；广西荔浦芋试验站项目(TS202113)作者简介：李慧楠(1995-),女,山西长治人,硕士,助理研究员,主要从事芋种质资源创新利用与遗传育种研究。(E-mail)Lihunan101@163.com通讯作者：何芳练,(E-mail)hefanglian048@163.com
更新日期/Last Update: 2025-12-18