[1]龚凤珍,吕俊婷,侯朝英,等.辣椒胶孢炭疽菌中糖苷水解酶蛋白的找寻及生物信息学分析[J].江苏农业学报,2025,(10):1926-1935.[doi:doi:10.3969/j.issn.1000-4440.2025.10.006]
　GONG Fengzhen,LYU Junting,HOU Chaoying,et al.Finding and bioinformatic analysis of glycoside hydrolase proteins in Colletotrichum gloeosporioides of pepper[J].,2025,(10):1926-1935.[doi:doi:10.3969/j.issn.1000-4440.2025.10.006]
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辣椒胶孢炭疽菌中糖苷水解酶蛋白的找寻及生物信息学分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年10期
页码:
1926-1935
栏目:
植物保护
出版日期:
2025-10-31

文章信息/Info

Title:
Finding and bioinformatic analysis of glycoside hydrolase proteins in Colletotrichum gloeosporioides of pepper
作者:
龚凤珍1; 2; 吕俊婷1; 侯朝英1; 韩长志1
(1.西南林业大学林学院/云南省森林灾害预警与控制重点实验室,云南昆明650224；2.岳阳市林业局,湖南岳阳414000)
Author(s):
GONG Fengzhen1; 2; LYU Junting1; HOU Chaoying1; HAN Changzhi1
(1.Forestry College of Southwest Forestry University/The Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Kunming 650224, China;2.Forestry Bureau of Yueyang City, Yueyang 414000, China)
关键词:
辣椒; 胶孢炭疽菌; 分泌蛋白; 糖苷水解酶
Keywords:
pepper; Colletotrichum gloeosporioides; secreted protein; glycoside hydrolase
分类号:
S436.418.11
DOI:
doi:10.3969/j.issn.1000-4440.2025.10.006
文献标志码:
A
摘要:
由胶孢炭疽菌(Colletotrichum gloeosporioides)引起的辣椒炭疽病,是制约辣椒产业高质量发展的重要病害,明确该病原菌中糖苷水解酶(GH)的特征与功能,对解析其致病机制具有重要意义。本研究采用生物信息学方法预测辣椒胶孢炭疽菌中糖苷水解酶蛋白,通过 ProtParam、ProtScale、SMART 等在线工具分析其理化性质、亲疏水性及保守结构域,利用MEGA 11软件构建系统发育树以解析遗传关系,并借助EggNOG-mapper工具进行直系同源簇(COG)蛋白功能注释。结果显示,在该病原菌中共预测到24个糖苷水解酶蛋白,多数为酸性、稳定的疏水性蛋白质,氨基酸数量集中于201 aa至400 aa,且含有糖苷水解酶保守结构域的糖苷水解酶主要归属于GH28亚家族；系统发育树将24个糖苷水解酶划分为3大类；COG功能注释表明,这些酶主要参与碳水化合物的转运与代谢,同时在蛋白质翻译后修饰及伴侣蛋白调控等生物过程中发挥作用。本研究明确了辣椒胶孢炭疽菌中糖苷水解酶的数量、理化特性及功能定位,为深入揭示该病原菌的致病分子机制、精准挖掘病原菌防治靶点、研发新型生物防治技术提供了重要理论支撑,同时对保障辣椒产业安全生产具有很高的实践价值。
Abstract:
Pepper anthracnose, caused by Colletotrichum gloeosporioides, is a major disease that restricts the high-quality development of the pepper industry. Clarifying the characteristics and functions of glycoside hydrolases (GHs) in this pathogen is of great significance for deciphering its pathogenic mechanism. In this study, bioinformatic methods were used to predict the GH proteins in Colletotrichum gloeosporioides isolated from pepper. Online tools including ProtParam, ProtScale, and SMART were employed to analyze the physicochemical properties, hydrophobicity, and conserved domains of these proteins. MEGA 11 software was utilized to construct a phylogenetic tree for analyzing genetic relationships, while the EggNOG-mapper tool was applied for functional annotation of orthologous group (COG) proteins. The results showed that a total of 24 GH proteins were predicted in this pathogen. Most of these proteins were acidic, stable, and hydrophobic, with amino acid lengths ranging from 201 aa to 400 aa. Moreover, glycoside hydrolases containing conserved GH domains were mainly classified into the GH28 subfamily. Based on the phylogenetic tree, the 24 glycoside hydrolases were classified into three major categories. COG functional annotation indicated that these enzymes were primarily involved in the transport and metabolism of carbohydrates, and also played roles in biological processes such as post-translational modification of proteins and chaperone protein regulation. This study clarifies the number, physicochemical properties, and functional localization of glycoside hydrolases in Colletotrichum gloeosporioides of pepper. It provides important theoretical support for in-depth exploration of the molecular pathogenic mechanism of this pathogen, accurate identification of targets for pathogen control, and development of novel biological control technologies. Meanwhile, it holds high practical value for ensuring the safe production of the pepper industry.

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备注/Memo

备注/Memo:
收稿日期:2025-05-23基金项目:云南省“兴滇英才支持计划”青年人才专项(YNWR-QNBJ-2020-188)；云南省研究生导师团队建设项目(2022100)；西南林业大学林学学科和重点实验室开放基金项目(LXXK-2025M06)作者简介:龚凤珍(1983-),湖南岳阳人,硕士,研究方向为资源利用与植物保护。(E-mail)349456513@qq.com通讯作者:韩长志,(E-mail)swfuhcz@163.com
更新日期/Last Update: 2025-11-17