[1]张婷,储俊东,王丹,等.急性低氧胁迫下中华绒螯蟹表皮基因表达谱分析[J].江苏农业学报,2025,(04):724-732.[doi:doi:10.3969/j.issn.1000-4440.2025.04.011]
　ZHANG Ting,CHU Jundong,WANG Dan,et al.Gene expression profile analysis of Eriocheir sinensis cuticle under acute hypoxia stress[J].,2025,(04):724-732.[doi:doi:10.3969/j.issn.1000-4440.2025.04.011]
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急性低氧胁迫下中华绒螯蟹表皮基因表达谱分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年04期
页码:
724-732
栏目:
畜牧兽医·水产养殖·益虫饲养
出版日期:
2025-04-30

文章信息/Info

Title:
Gene expression profile analysis of Eriocheir sinensis cuticle under acute hypoxia stress
作者:
张婷1; 2; 储俊东1; 2; 王丹1; 2; 周军2; 邓燕飞2; 张慧敏2; 许建和1; 李旭光2
(1.江苏海洋大学海洋科学与水产学院,江苏连云港222005；2.江苏省淡水水产研究所/农业农村部淡水虾蟹遗传育种与养殖重点实验室,江苏南京210017)
Author(s):
ZHANG Ting1; 2; CHU Jundong1; 2; WANG Dan1; 2; ZHOU Jun2; DENG Yanfei2; ZHANG Huimin2; XU Jianhe1; LI Xuguang2
(1.School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China;2.Freshwater Fisheries Research Institute of Jiangsu Province/Key Laboratory of Genetic Breeding and Cultivation for Freshwater Crustacean, Ministry of Agriculture and Rural Affairs, Nanjing 210017, China)
关键词:
中华绒螯蟹; 急性低氧胁迫; 蜕壳后期; 表皮基因; 转录组分析
Keywords:
Eriocheir sinensis; acute hypoxia stress; post-molt stage; cuticle genes; transcriptome analysis
分类号:
TP391；S565.1
DOI:
doi:10.3969/j.issn.1000-4440.2025.04.011
文献标志码:
A
摘要:
为探究急性低氧胁迫下蜕壳后期中华绒螯蟹表皮基因表达响应特征,本研究通过使用Illumina第二代测序技术,检测了对照与急性低氧胁迫处理[(1.0±0.1 mg/L处理1 h)]处于蜕壳后期的中华绒螯蟹表皮基因表达谱差异。结果表明,急性低氧胁迫处理中华绒螯蟹蜕壳后期表皮基因与对照相比有691个差异表达,主要富集在角质层结构成分、几丁质结合等生理活动和氮代谢、甘油酯代谢、糖酵解途径等代谢通路,从中筛选出9个显著差异表达基因，并进行了实时荧光定量PCR验证。本研究结果初步阐明了处于蜕壳后期的中华绒螯蟹表皮应对急性低氧胁迫的分子机制,为后续深入开展低氧胁迫对中华绒螯蟹蜕壳生长的影响奠定了基础。
Abstract:
To investigate the gene expression response in the cuticle of Eriocheir sinensis during the post-molt stage under acute hypoxia stress, this study utilized Illumina second-generation sequencing technology to detect the gene expression profiles between the control and the acute hypoxia stress treatment, namely (1.0±0.1) mg/L for 1 h. The results showed that compared with the control, a total of 691 differential expressed genes were identified in the acute hypoxia stress treatment. These differentially expressed genes were mainly enriched in physiological activities such as cuticle structural components, chitin binding, and metabolic pathways such as nitrogen metabolism, glyceride metabolism, and glycolysis pathway. Nine significantly differentially expressed genes were screened and verified by real-time fluorescence quantitative PCR. The results of this study preliminarily clarify the molecular response mechanism of cuticle in E. sinensis at the post-molt stage to acute hypoxia stress, laying the foundation for further investigation into the effects of hypoxia stress on the molting and growth of E. sinensis.

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备注/Memo

备注/Memo:
收稿日期：2024-08-30基金项目：江苏省种业振兴“揭榜挂帅”项目[JBGS(2021)031、JBGS(2021)125]；“十四五”国家重点研发计划项目(2022YFD2400700)；江苏省碳达峰碳中和科技创新专项资金项目(SBE2022350035)；江苏海洋大学研究生科研与实践创新计划项目(KYCX2023-103)作者简介：张婷(1999-),女,山西大同人,硕士研究生,主要从事甲壳动物养殖研究。(E-mail)braveting04@163.com通讯作者：李旭光,(E-mail)xuguangli1981@163.com
更新日期/Last Update: 2025-05-26