[1]马澜,邱黛玉,巫蓉,等.栽培模式对党参根际土壤微生物功能多样性的影响[J].江苏农业学报,2023,(05):1132-1139.[doi:doi:10.3969/j.issn.1000-4440.2023.05.005]
　MA Lan,QIU Dai-yu,WU Rong,et al.Effects of cultivation modes on microbial functional diversity in the rhizosphere soil of Codonopsis pilosula[J].,2023,(05):1132-1139.[doi:doi:10.3969/j.issn.1000-4440.2023.05.005]
点击复制

栽培模式对党参根际土壤微生物功能多样性的影响()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年05期
页码:
1132-1139
栏目:
耕作栽培·资源环境
出版日期:
2023-08-31

文章信息/Info

Title:
Effects of cultivation modes on microbial functional diversity in the rhizosphere soil of Codonopsis pilosula
作者:
马澜1; 邱黛玉1; 巫蓉2; 郭建陈1; 宋世杰1; 王雪1; 陈垣1
（1.甘肃农业大学农学院/甘肃省干旱生境作物学重点实验室,甘肃兰州730070；2.南京市六合区中医院,江苏南京211599）
Author(s):
MA Lan1; QIU Dai-yu1; WU Rong2; GUO Jian-chen1; SONG Shi-jie1; WANG Xue1; CHEN Yuan1
（1.College of Agronomy, Gansu Agricultural University / Gansu Province Key Laboratory of Arid Habitat Crop Science， Lanzhou 730070， China；2.Luhe District Traditional Chinese Medicine Hospital， Nanjing 211599， China）
关键词:
党参; 连作障碍; 轮作; 根际微生物; 碳源利用
Keywords:
Codonopsis pilosula; continuous cropping obstacle; crop rotation; rhizosphere microorganisms; carbon source utilization
分类号:
S567.5+3
DOI:
doi:10.3969/j.issn.1000-4440.2023.05.005
文献标志码:
A
摘要:
为探究连作与轮作2种栽培模式对党参根际土壤微生物群落结构及功能多样性的影响，本研究以党参连作和黄芪-党参轮作根际为研究对象，采用 Biolog-Eco技术分析不同栽培模式下根际土壤微生物群落碳源代谢特征及功能多样性差异。结果表明2种栽培模式下，党参根际土壤细菌、真菌、放线菌数量及微生物总数随着党参生长发育均呈先升后降趋势；与轮作相比，连作根际土壤真菌数量显著增加，而细菌及放线菌数量、微生物总数显著降低。党参根际土壤微生物总体代谢活性能力由大到小依次轮作始期、轮作采收期、连作始期、连作采收期；连作采收期根际土壤微生物丰富度降低且分布均匀度较差，对碳源利用能力较弱。4类碳源（1种氨基酸类、1种糖类、2种酯类、1种酸类）与第1主成分（PC1）有较高正相关性，且这些碳源均为有机酸或糖类的衍生物，表明党参连作与轮作根际土壤微生物对碳源利用存在显著差异。本研究结果可为党参连作障碍干预及合理轮作提供参考。
Abstract:
In order to explore the effects of continuous cropping and rotation on the microbial community structure and functional diversity in the rhizosphere soil of Codonopsis pilosula, the rhizosphere of Codonopsis pilosula continuous cropping and Astragalus membranaceus-Codonopsis pilosula rotation was used as the research object. Biolog-Eco technology was used to analyze the differences in carbon metabolism characteristics and functional diversity of soil microbial community under different cultivation methods. The results showed that the number of bacteria, fungi, actinomycetes and the total number of microorganisms in the rhizosphere soil of Codonopsis pilosula increased first and then decreased with the growth and development of Codonopsis pilosula under the two cultivation methods. Compared with rotation, the number of fungi in continuous cropping rhizosphere soil increased significantly, while the number of bacteria and actinomycetes and the total number of microorganisms decreased significantly. The overall metabolic activity ability of microorganisms in the rhizosphere soil of Codonopsis pilosula from large to small in turn was the beginning of ratation, the harvesting period of rotation, the beginning of continuous cropping, and the harvesting period of continuous cropping. In the harvesting period of continuous cropping, the rhizosphere soil microbial richness decreased and the distribution uniformity was poor, and the carbon utilization ability was weak. Four types of carbon sources (including one amino acid, one sugar, two esters, and one acid) had a high positive correlation with the first principal component (PC1), and these carbon sources were derivatives of organic acids or sugars. These results indicated that there were significant differences in the utilization of carbon sources by microorganisms in the rhizosphere soil of continuous cropping and rotation of Codonopsis pilosula. The results of this study can provide reference for continuous cropping obstacle intervention and reasonable rotation of Codonopsis pilosula.

参考文献/References:

[1]国家药典委员会. 中华人民共和国药典（一部）[M]. 北京：中国医药科技出版社, 2020：281-282.
[2]张重义,尹文佳,李娟,等. 地黄连作的生理生态特性[J]. 植物生态学报, 2010, 34(5):547-554.
[3]张欢强,慕小倩,梁宗锁,等. 附子连作障碍效应初步研究[J]. 西北植物学报, 2007，27(10):2112-2115.
[4]陈慧,杨志玲,袁志林,等. 白术连作根际土壤的理化性质及微生物区系变化[J]. 植物资源与环境学报, 2014, 23(1): 24-29.
[5]凡续晨,刘强,徐钰惟,等. 中药材连作障碍研究进展[J]. 四川农业科技, 2021(11):28-30.
[6]YANG P, LUO Y, GAO Y, et al. Soil properties, bacterial and fungal community compositions and the key factors after 5-year continuous monocropping of three minor crops.[J]. PLoS One, 2020,15(8):e0237164.
[7]刘诗蓉,王红兰,孙辉,等. 半夏连作对根际土壤微生物群落的影响研究[J]. 中草药, 2022, 53(4):1148-1155.
[8]保丽美,丁亚芳,魏云林,等. 三七连作与休闲土壤真菌群落组成与多样性分析[J]. 中药材, 2021, 44(1):7-12.
[9]李润根,曾慧兰,曾建梅. 基于高通量测序的连作百合土壤细菌群落多样性研究[J].宜春学院学报, 2021, 43(3):85-91.
[10]葛晓颖,孙志刚,李涛,等.设施番茄连作障碍与土壤芽孢杆菌和假单胞菌及微生物群落的关系分析[J].农业环境科学学报,2016,35(3):514-523.
[11]吴艳飞,张雪艳,李元,等.轮作对黄瓜连作土壤环境和产量的影响[J].园艺学报,2008,35(3):357-362.
[12]杨祥田,周翠,李建辉,等.不同轮作方式下大棚草莓产量及土壤生物学特性[J].中国生态农业学报, 2010, 18(2): 312-315.
[13]于高波,吴凤芝,周新刚. 小麦、毛苕子与黄瓜轮作对土壤微生态环境及产量的影响[J].土壤学报,2011,48(1):175-184.
[14]邱黛玉,杜毛笑,巫蓉,等. 连作障碍对党参根际土壤微环境的影响[J].中国野生植物资源,2022,41(5):12-17.
[15]张艳如.党参高产优质栽培技术探讨[J].种子科技,2021,39(7):41-42.
[16]RUTGERS M, WOUTERSE M, DROST S M, et al. Monitoring soil bacteria with community-level physiological profiles using Biolog ECO-Plates in the Netherlands and Europe［J］.Applied Soil Ecology, 2016,97(1): 23-35.
[17]STEFANOWICZ A M, KAPUSTA P, STANEK M, et al. Invasive plant Reynoutria japonica produces large amounts of phenolic com-pounds and reduces the biomass but not activity of soil microbial communities［J］. Science of the Total Environment, 2021, 767(5):145439.
[18]李明智,张宇,梅荣武,等. Biolog ECO分析活性污泥微生物功能多样性特征［J］.环境科学与技术,2016,39(6):55-58，100.
[19]ZAK J C, WILLING M R, MOORHERD D L, et al. Functional diversity of microbial communities: A quantitative approach[J]. Soil Biol Biochem，1994, 26:1101-1108.
[20]席劲瑛,胡洪营,钱易. Biolog方法在环境微生物群落研究中的应用[J].微生物学报,2003,43(1):138-141.
[21]汤有宏,李红歌,李晓欢. 基于 Biolog ECO 技术对贮存过程中大曲微生物多样性变化规律的研究[J].酿酒,2015,42(1):75-78.
[22]江聪,简小枚,杜勇,等.若尔盖高寒草地微地形的土壤微生物群落多样性特征[J].福州大学学报(自然科学版),2018,46(6):831-838.
[23]郭靖.对K-means聚类算法欧氏距离加权系数的研究[J].网络安全技术与应用, 2016(10):74-75.
[24]王长义,郝振萍,陈丹艳,等.设施土壤连作障碍产生原因及防治方法综述[J].江苏农业科学, 2020, 48(8):1-6.
[25]DONG L L, XU G, FENG G Q, et al. Soil bacterial and fungal community dynamics in relation to Panax notoginseng death rate in a continuous cropping system[J]. Scientific Reports,2016,6(1):31802.
[26]白容霖,刘学敏,刘伟成.吉林省人参根腐病病原真菌种类的研究[J].植物病理学报,1999,29(3):285.
[27]郝晓芬,王根全,郭二虎,等.连作、轮作对谷子根际细菌群落结构的影响[J].农业环境科学学报,2022,41(3):585-596.
[28]李冰圳.连作及轮作对蒙古黄芪根际微生物多样性及其品质的影响[D].呼和浩特:内蒙古大学. 2019.
[29]HERMANS S M, BUCKLEY H L, CASE B S, et al. Using soil bacterial communities to predict physico-chemical variables and soil quality[J]. Microbiome, 2020, 8(1):79.
[30]OKAZAKI K, TSURUMARU H, HASHIMOTO M, et al. Community analysis-based screening of plant growth-promoting bacteria for sugar beet[J]. Microbes and Environments, 2021,36(2):ME20137.
[31]BERG G, SMALLA K. Plant species and soil type cooperatively shape the structure and function of microbial communities in the rhizosphere[J]. FEMS Microbiology Ecology, 2009, 68(1) : 1-13.
[32]LU L H, YIN S X, LIU X, et al. Fungal networks in yield-invigorating and -debilitating soils induced by prolonged potato monoculture[J]. Soil Biology and Biochemistry, 2013, 65:186-194.
[33]李戌清,张雅,田忠玲,等.茄子连作与轮作土壤养分、酶活性及微生物群落结构差异分析[J].浙江大学学报(农业与生命科学版), 2017, 43(5):561-569.
[34]邹春娇,齐明芳,马建,等.Biolog-ECO解析黄瓜连作营养基质中微生物群落结构多样性特征[J].中国农业科学,2016,49(5):942-951.
[35]李春格,李晓鸣,王敬国.大豆连作对土体和根际微生物群落功能的影响[J].生态学报,2006,26(4):1144-1150.
[36]马玲,马琨,杨桂丽,等.马铃薯连作栽培对土壤微生物多样性的影响[J].中国生态农业学报,2015,23(5):589-596.
[37]万平平,刘胡楠,张文婕,等.不同园林植物根际土壤微生物群落代谢差异性研究[J].山东农业大学学报(自然科学版),2022,53(2):246-252.

相似文献/References:

[1]周开胜.厌氧还原土壤灭菌法抑制西瓜专化型尖孢镰刀菌[J].江苏农业学报,2015,(05):1006.[doi:doi:10.3969/j.issn.1000-4440.2015.05.010]
　ZHOU Kai-sheng.Anaerobic soil disinfestation, an effective way to control watermelon fusarium wilt caused by Fusarium oxysporum f. sp. niveum[J].,2015,(05):1006.[doi:doi:10.3969/j.issn.1000-4440.2015.05.010]
[2]刘勇,刘燕,胡茂飞,等.头花蓼水浸提液的化感自毒作用[J].江苏农业学报,2018,(03):520.[doi:doi:10.3969/j.issn.1000-4440.2018.03.006]
　LIU Yong,LIU Yan,HU Mao-fei,et al.Research on allelopathic autotoxicity of water extracts of Polygonum capitatum[J].,2018,(05):520.[doi:doi:10.3969/j.issn.1000-4440.2018.03.006]
[3]徐彬,徐健,祁建杭,等.江苏省设施蔬菜连作障碍土壤理化及生物特征[J].江苏农业学报,2019,(05):1124.[doi:doi:10.3969/j.issn.1000-4440.2019.05.018]
　XU Bin,XU Jian,QI Jian-hang,et al.Soil physical, chemical and biological characteristic analysis of long-term vegetable monoculture in greenhouse in Jiangsu province[J].,2019,(05):1124.[doi:doi:10.3969/j.issn.1000-4440.2019.05.018]
[4]孟佳丽,吴绍军,沈虹,等.不同药剂处理对西瓜连作土壤的影响[J].江苏农业学报,2021,(03):651.[doi:doi:10.3969/j.issn.1000-4440.2021.03.013]
　MENG Jia-li,WU Shao-jun,SHEN Hong,et al.Effects of different chemical treatments on continuous cropping soil of watermelon[J].,2021,(05):651.[doi:doi:10.3969/j.issn.1000-4440.2021.03.013]
[5]林超,邱黛玉,陈垣,等.党参根际土壤浸提液对其种子萌发和幼苗生长的影响[J].江苏农业学报,2022,38(04):900.[doi:doi:10.3969/j.issn.1000-4440.2022.04.005]
　LIN Chao,QIU Dai-yu,CHEN Yuan,et al.Effects of aqueous extracts from rhizosphere soil of Codonopsis pilosula on seed germination and seedling growth[J].,2022,38(05):900.[doi:doi:10.3969/j.issn.1000-4440.2022.04.005]
[6]宋燕芳,彭彤,马少兰,等.枸杞叶片酚酸类物质的自毒作用分析[J].江苏农业学报,2024,(02):213.[doi:doi:10.3969/j.issn.1000-4440.2024.02.003]
　SONG Yan-fang,PENG Tong,MA Shao-lan,et al.Analyzing the autotoxicity of phenolic acids from Lycium barbarum L. leaves[J].,2024,(05):213.[doi:doi:10.3969/j.issn.1000-4440.2024.02.003]

备注/Memo

备注/Memo:
收稿日期：2022-09-18 基金项目：国家自然科学基金项目（31960395）；国家重点研发计划项目（2018YFC17060301）;甘肃省优秀研究生创新之星项目（2022CXZXS-019）作者简介：马澜（1998-），女，甘肃嘉峪关人，硕士研究生，主要从事药用植物资源利用。（E-mail）454159427@qq.com 通讯作者：邱黛玉，（E-mail）qiudy@gsau.edu.cn
更新日期/Last Update: 2023-09-13