[1]宋燕芳,彭彤,马少兰,等.枸杞叶片酚酸类物质的自毒作用分析[J].江苏农业学报,2024,(02):213-222.[doi:doi:10.3969/j.issn.1000-4440.2024.02.003]
　SONG Yan-fang,PENG Tong,MA Shao-lan,et al.Analyzing the autotoxicity of phenolic acids from Lycium barbarum L. leaves[J].,2024,(02):213-222.[doi:doi:10.3969/j.issn.1000-4440.2024.02.003]
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枸杞叶片酚酸类物质的自毒作用分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年02期
页码:
213-222
栏目:
遗传育种·生理生化
出版日期:
2024-02-25

文章信息/Info

Title:
Analyzing the autotoxicity of phenolic acids from Lycium barbarum L. leaves
作者:
宋燕芳1; 2; 彭彤1; 2; 马少兰3; 马彩霞4; 高娜1; 2; 李凯乐1; 2; 张传继1; 2; 蒲美运1; 2; 纳小凡1; 2
(1.兰州大学生命科学学院,甘肃兰州730000；2.细胞活动与逆境适应教育部重点实验室,甘肃兰州730000；3.彭阳县第一中学,宁夏固原756500；4.宁夏大学生命科学学院,宁夏银川750021)
Author(s):
SONG Yan-fang1; 2; PENG Tong1; 2; MA Shao-lan3; MA Cai-xia4; GAO Na1; 2; LI Kai-le1; 2; ZHANG Chuan-ji1; 2; PU Mei-yun1; 2; NA Xiao-fan1; 2
(1.School of Life Sciences, Lanzhou University, Lanzhou 730000, China；2.Key Laboratory of Cell Activities and Stress Adaptations， Ministry of Education, Lanzhou 730000, China；3.Pengyang No.1 Middle School, Guyuan 756500, China；4.School of Life Sciences, Ningxia University, Yinchuan 750021, China)
关键词:
枸杞; 连作障碍; 自毒物质; 叶片; 酚酸
Keywords:
Lycium barbarum L.; continuous cropping obstacle; autotoxins; leaf; phenolic acid
分类号:
S567.19
DOI:
doi:10.3969/j.issn.1000-4440.2024.02.003
摘要:
自毒作用是植物连作障碍形成的主要原因之一,严重影响植物生长和粮食生产。前期研究结果表明,土壤微生物多样性下降、土传病害加重是枸杞园连作障碍形成的重要原因,但枸杞残体的自毒作用及其物质基础目前仍不明确。为探讨自毒物质的主要来源,本研究对比分析了枸杞(Lycium barbarum L.)叶、茎、根等组织浸提液对枸杞幼苗生长的影响。浓度梯度试验结果表明,叶片浸提液的抑制效应高于根、老根皮及茎浸提液。向无枸杞种植历史的农田土壤中添加枸杞叶片干粉能够显著抑制枸杞幼苗生长及叶片光合作用,证实了枸杞叶片具有自毒作用。联合UPLC-QTOF-MS和GC-TOF-MS技术对生长季末期枸杞叶片进行代谢组分析,共鉴定出有机酸112种、醇类41种、氨基酸37种、醛酮类28种和糖类48种。对其中24种有机酸及衍生物的分析发现,20种有机物能够不同程度抑制枸杞幼苗初生根生长。其中水杨酸、邻苯二甲酸、对羟基苯甲酸和香豆素在浓度低至10 μmol/L时仍具显著抑制效应。UPLC结果进一步显示,长期连作导致枸杞园土壤香豆素、水杨酸、苯甲酸、阿魏酸和香豆酸积累。此外,土壤总酚酸含量也随种植年限增加显著增加。这些结果表明土壤中枸杞叶片酚酸能够在长期连作下积累并诱发自毒作用,这为进一步探索枸杞连作障碍形成机制提供了研究基础。
Abstract:
Autotoxicity is one of the main reasons for the formation of continuous cropping barriers, which seriously affects plant growth and food production. The results of previous studies showed that the decrease of soil microbial diversity and the aggravation of soil-borne diseases were the important reasons for the formation of continuous cropping obstacles. But the self-toxic effects and the material basis of residues of Lycium barbarum were still unclear. In order to study the main sources of autotoxins, the effects of leaf, stem and root extracts of Lycium barbarum L. on the growth of Lycium barbarum L. seedlings were studied. The results of concentration gradient test showed that the inhibition effect of leaf extract was higher than that of root, old root bark and stem extract. The growth and photosynthesis of Lycium barbarum L. seedlings were significantly inhibited by adding Lycium barbarum L. leaf powder to the farmland soil without Lycium barbarum L. planting history. It was proved that the leaves of Lycium barbarum L. had self-toxicity. Combined with UPLC-QTOF-MS and GC-TOF-MS for metabolome analysis of Lycium barbarum L. leaves at the end of growing season, a total of 112 organic acids, 41 alcohols, 37 amino acids, 28 aldehydes and ketones and 48 sugars were identified. Analysis of 24 kinds of organic acids and derivatives showed that 20 kinds of organic compounds could inhibit the primary root growth of Lycium barbarum L. seedlings. Salicylic acid, phthalic acid, p-hydroxybenzoic acid, and coumarin still showed significant inhibitory effects at concentrations as low as 10 μmol/L. UPLC results further showed that long-term continuous cropping led to the accumulation of coumarin, salicylic acid, benzoic acid, ferulic acid and coumaric acid. In addition, the content of total phenolic acids in soil increased significantly with the increase of planting years. These results indicated that phenolic acids could accumulate and induce autotoxicity in the leaves of Lycium barbarum L. under long-term continuous cropping, which provided a basis for further research on the mechanism of continuous cropping failure.

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备注/Memo

备注/Memo:
收稿日期:2022-12-31基金项目:甘肃省自然科学基金项目(22JR5RA461)作者简介:宋燕芳(1998-),女,内蒙古呼和浩特人,硕士研究生,主要从事植物与微生物互作研究。(E-mail)15771555600@163.com通讯作者:纳小凡,(E-mail)naxf@lzu.edu.cn
更新日期/Last Update: 2024-03-17