[1]徐筋燕,何晓兰,邵明灿,等.不同连作年限栝楼根际土壤微生物群落多样性分析[J].江苏农业学报,2023,(05):1140-1150.[doi:doi:10.3969/j.issn.1000-4440.2023.05.006]
　XU Jin-yan,HE Xiao-lan,SHAO Ming-can,et al.Analysis of microbial community diversity in rhizosphere soil of continuous cropping Trichosanthes kirilowii[J].,2023,(05):1140-1150.[doi:doi:10.3969/j.issn.1000-4440.2023.05.006]
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不同连作年限栝楼根际土壤微生物群落多样性分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年05期
页码:
1140-1150
栏目:
耕作栽培·资源环境
出版日期:
2023-08-31

文章信息/Info

Title:
Analysis of microbial community diversity in rhizosphere soil of continuous cropping Trichosanthes kirilowii
作者:
徐筋燕1; 何晓兰1; 邵明灿1; 万云龙2; 刁亚梅3; 杨中海4; 郭书巧1
（1.江苏省农业科学院经济作物研究所,江苏南京210014；2.江苏省农业科学院蔬菜研究所,江苏南京210014；3.江苏农林职业技术学院农学园艺学院,江苏句容212400；4.涟水雨果农业发展有限公司,江苏淮安223411）
Author(s):
XU Jin-yan1; HE Xiao-lan1; SHAO Ming-can1; WAN Yun-long2; DIAO Ya-mei3; YANG Zhong-hai4; GUO Shu-qiao1
(1.Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;3.School of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Jurong 212400, China;4.Lianshui Yuguo Agricultural Development Co., Ltd., Huai’an 223411, China)
关键词:
栝楼; 连作; 根际土壤; 微生物群落; 土壤理化性质; 高通量测序
Keywords:
Trichosanthes kirilowii; continuous cropping; rhizosphere soil; microbial community; physicochemical properties of the soil; high-throughput sequencing
分类号:
S567.23+9
DOI:
doi:10.3969/j.issn.1000-4440.2023.05.006
文献标志码:
A
摘要:
为明确连作对栝楼根际土壤微生物群落的影响和栝楼连作障碍产生的原因,以连作2年和连作4年的栝楼根际土壤为研究对象,进行土壤理化性质测定以及土壤细菌16S和真菌ITS高通量测序。土壤理化性质测定结果表明,与连作2年的栝楼根际土壤样本相比,连作4年的栝楼根际土壤样本中有机碳含量下降；与对照土壤样本和连作2年的栝楼根际土壤样本相比,连作4年的栝楼根际土壤样本阳离子交换量（CEC）下降,说明土壤保肥能力已下降。细菌群落α多样性分析结果显示,栝楼连作对细菌群落的影响相对较小。细菌群落结构分析结果表明,与对照土壤样本相比,栝楼连作根际土壤中一些具有重要功能的细菌门相对丰度下降。与对照土壤样本相比,连作2年和连作4年的栝楼根际土壤样本中真菌群落群落结构和组成发生明显变化,一些有害真菌属如Stagonosporopsis、Rhizoctonia和Colletotrichum的相对丰度大幅增加。典型相关分析结果表明,硝态氮含量、全磷含量和速效磷含量是栝楼根际土壤微生物群落多样性的主要影响因素；pH值、阳离子交换量是CK土壤微生物群落多样性的主要影响因素。
Abstract:
To clarify the effects of continuous cropping on the rhizosphere soil microbial community of Trichosanthes kirilowii and the causes of continuous cropping obstacles, rhizosphere soil samples of Trichosanthes kirilowii after continuous cropping for two and four years were used for analyzing in this study. The physicochemical properties of the soil samples were determined, and the bacterial 16S library and fungal ITS library were constructed and sequenced by high-throughput sequencing technology. The results of physicochemical properties showed that compared with the rhizosphere soil samples of Trichosanthes kirilowii after continuous cropping for two years, the organic carbon content in the rhizosphere soil samples of Trichosanthes kirilowii after continuous cropping for four years decreased. Compared with the control soil samples and the rhizosphere soil samples of Trichosanthes kirilowii after continuous cropping for two years, the cation exchange capacity (CEC) of soil samples began to decrease after four years of continuous cropping, indicating that the soil fertility retention capacity began to decrease. The results of α-diversity of bacterial communities showed that the continuous cropping of Trichosanthes kirilowii had a relatively small effect on bacterial community. However, the relative abundance of some functional bacteria in the rhizosphere soil samples of continuous cropping Trichosanthes kirilowii decreased campared with that in the control soil samples. Compared with the control soil samples, the structure and composition of fungal communities in the rhizosphere soil samples of Trichosanthes kirilowii after continuous cropping for two years and four years changed significantly, and the relative abundance of some harmful fungi such as Stagonosporopsis, Rhizoctonia and Colletotrichum increased significantly. The results of canonical correlation analysis showed that nitrate nitrogen content, total phosphorus content and available phosphorus content were the main influencing factors of microbial community diversity in rhizosphere soil of Trichosanthes kirilowii, and pH and CEC were the main influencing factors of soil microbial community diversity in CK.

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备注/Memo

备注/Memo:
收稿日期：2022-09-19 基金项目：国家自然科学基金青年项目（31901559）；江苏省农业科技自主创新基金项目［CX(21)3167］；江苏省苏北科技专项（SZ-HA2021034）作者简介：徐筋燕（1985-）,女,江苏昆山人,助理研究员,主要从事栝楼遗传育种研究。（Tel）025-84390290；（E-mail）xujinyan0610@126.com 通讯作者：郭书巧,（E-mail）sg2015@jaas.ac.cn
更新日期/Last Update: 2023-09-13