[1]贺丹,曹健康,何松林,等.芍药属植物远缘杂交亲和性及其雌蕊的生理响应机制[J].江苏农业学报,2023,(03):822-830.[doi:doi:10.3969/j.issn.1000-4440.2023.03.024]
　HE Dan,CAO Jian-kang,HE Song-lin,et al.Distant hybrid compatibility of Paeonia and its physiological response mechanism of pistil[J].,2023,(03):822-830.[doi:doi:10.3969/j.issn.1000-4440.2023.03.024]
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芍药属植物远缘杂交亲和性及其雌蕊的生理响应机制()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年03期
页码:
822-830
栏目:
园艺
出版日期:
2023-06-30

文章信息/Info

Title:
Distant hybrid compatibility of Paeonia and its physiological response mechanism of pistil
作者:
贺丹1; 2; 曹健康1; 何松林2; 符真珠3; 华超1; 张明星1; 王政1
（1.河南农业大学风景园林与艺术学院，河南郑州450002；2.河南科技学院园艺园林学院，河南新乡453003；3.河南省农业科学院，河南郑州450002）
Author(s):
HE Dan1; 2; CAO Jian-kang1; HE Song-lin2; FU Zhen-zhu3; HUA Chao1; ZHANG Ming-xing1; WANG Zheng1
(1.College of Landscape Architecture and Art, Henan Agricultural University, Zhengzhou 450002, China;2.College of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China;3.Henan Academy of Agricultural Sciences, Zhengzhou 450002, China)
关键词:
芍药; 牡丹; 远缘杂交; 保护酶活性; 胼胝质; β-1; 3-葡聚糖酶活性
Keywords:
Paeonia lactiflora; Paeonia suffruticosa distant hybridization; protective enzyme activity; callose; β-1; 3-glucanase activity
分类号:
S682.1
DOI:
doi:10.3969/j.issn.1000-4440.2023.03.024
文献标志码:
A
摘要:
为探究不同芍药属植物远缘杂交的亲和性,筛选出亲和性较好的杂交组合，以芍药老来红（LLH）、蝶恋花（DLH）、鲁红（LH）、莲台（LT）、星光灿烂(XGCL)、遍地红(BDH)、Kansas、Venus和Peter Brand 9个品种为母本，以牡丹凤丹白（FDB）为父本进行远缘杂交。测定授粉后不同时间（1 h、2 h、4 h、8 h、12 h、24 h）雌蕊中的保护酶[过氧化物酶（POD）、超氧化物歧化酶（SOD）]活性、胼胝质（β-1,3-葡聚糖）含量及β-1,3-葡聚糖酶活性，并对授粉后花粉粒萌发及花粉管生长情况进行荧光显微观察。结果表明，授粉后2 h观察到花粉粒开始萌发，BDH×FDB、Kansas×FDB和Peter Brand×FDB 3个组合的花粉粒萌发相对较晚。随后在花粉管的生长过程中，胼胝质不断沉积，花粉管开始出现扭曲、缠绕等现象。授粉后24 h，LLH×FDB、LH×FDB和Kansas×FDB 3个组合可以观察到花粉管进入柱头，但未进入子房。POD、SOD活性在9个组合间及同一组合授粉后不同时间表现出显著差异，当POD、SOD活性较高时，花粉粒萌发较快且花粉管正常生长。胼胝质含量及β-1,3-葡聚糖酶活性在不同组合间差异显著，高含量的胼胝质及低活性的β-1,3-葡聚糖酶会导致花粉管出现扭曲缠绕、肿胀等异常现象。国内的芍药品种中，LLH×FDB、LH×FDB组合的亲和性较好，BDH×FDB组合的亲和性最差；国外的芍药品种中，Kansas×FDB组合的亲和性相对较好，Peter Brand×FDB组合的亲和性最差。
Abstract:
In order to explore the compatibility of distant hybridization of different Paeonia lactiflora plants and screen out the hybrid combinations with better compatibility, P. lactiflora Laolaihong (LLH), Dielianhua (DLH), Luhong (LH), Liantai (LT), Xingguangcanlan (XGCL), Biandihong (BDH), Kansas, Venus and Peter Brand were used as female parents, and P. suffruticosa Fengdanbai (FDB) was used as male parent for distant hybridization. The activities of peroxidase (POD) and superoxide dismutase (SOD), the content of callose (β-1,3-glucan) and the activity of β-1,3-glucanase were determined at different times (1 h, 2 h, 4 h, 8 h, 12 h, 24 h) in the pistil after pollination. The germination of pollen grains and the growth of pollen tubes after pollination were observed by fluorescence microscopy. The results showed that the pollen grains began to germinate at two hours after pollination, and the pollen grains of BDH×FDB, Kansas×FDB and Peter Brand×FDB germinated relatively late. Subsequently, during the growth of the pollen tubes, the callose was continuously deposited, and the pollen tubes began to twist. At 24 h after pollination, the pollen tubes could be observed to enter the stigma but not the ovary in the three combinations of LLH×FDB, LH×FDB and Kansas×FDB. Activities of POD and SOD changed significantly in nine combinations and at different times after pollination in the same combination. When the activities of POD and SOD were higher, the pollen grains germinated faster and the pollen tubes grew normally. The callose content and β-1,3-glucanase activity were significantly different among different combinations. High callose content and low activity of β-1,3-glucanase could lead to abnormal phenomena such as distortion, winding and swelling of pollen tubes. Among domestic varieties, the combinations of LLH×FDB and LH×FDB had better affinity, and BDH×FDB combination had the worst affinity. Among foreign varieties, the combination of Kansas×FDB had relatively good affinity, and the combination of Peter Brand×FDB had the worst affinity.

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备注/Memo

备注/Memo:
收稿日期：2022-07-04 基金项目：河南省高等学校重点科研项目（23A220001）；河南农业大学2021-2022年度“百千万”社会服务基金项目（30802210）；国家自然科学基金项目（31600568、31870698）作者简介：贺丹（1983-），女，河南新乡人，博士，副教授，主要从事园林植物育种研究。(E-mail)dandan990111@163.com 通讯作者：何松林，(E-mail)hsl213@yeah.net
更新日期/Last Update: 2023-07-11