[1]高晓晓,涂丽琴,孙枫,等.江苏蚕豆三叶草黄脉病毒的分子鉴定及全基因组结构特征分析[J].江苏农业学报,2022,38(05):1203-1210.[doi:doi:10.3969/j.issn.1000-4440.2022.05.006]
　GAO Xiao-xiao,TU Li-qin,SUN Feng,et al.Molecular identification and genomic characterization of clover yellow vein virus isolated from broad bean in Jiangsu province[J].,2022,38(05):1203-1210.[doi:doi:10.3969/j.issn.1000-4440.2022.05.006]
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江苏蚕豆三叶草黄脉病毒的分子鉴定及全基因组结构特征分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年05期
页码:
1203-1210
栏目:
植物保护
出版日期:
2022-10-31

文章信息/Info

Title:
Molecular identification and genomic characterization of clover yellow vein virus isolated from broad bean in Jiangsu province
作者:
高晓晓1; 2; 涂丽琴2; 孙枫2; 李硕2; 崔晓艳3; 陈新3; 章松柏1; 季英华2
（1.农林病虫害预警与调控湖北省工程技术研究中心/长江大学，湖北荆州434025；2.江苏省农业科学院植物保护研究所，江苏南京210014；3.江苏省农业科学院经济作物研究所，江苏南京210014）
Author(s):
GAO Xiao-xiao1; 2; TU Li-qin2; SUN Feng2; LI Shuo2; CUI Xiao-yan3; CHEN Xin3; ZHANG Song-bai1; JI Ying-hua2
(1.Hubei Engineering Research Center for Pest Forewarning and Management/Yangtze University, Jingzhou 434025， China；2.Institute of Plant Protection， Jiangsu Academy of Agricultural Sciences， Nanjing 210014， China；3.Institute of Industrial Crops， Jiangsu Academy of Agricultural Sciences， Nanjing 210014， China)
关键词:
三叶草黄脉病毒; 蚕豆; 全基因组; 序列分析
Keywords:
clover yellow vein virus; broad bean; whole genome; sequence analysis
分类号:
S435.23
DOI:
doi:10.3969/j.issn.1000-4440.2022.05.006
文献标志码:
A
摘要:
三叶草黄脉病毒（Clover yellow vein virus, ClYVV）是马铃薯Y病毒属的重要成员，侵染多种豆科作物并造成严重危害。为明确从江苏省盐城市采集的蚕豆样品中检测出的ClYVV全基因组序列以及基因组结构特征，通过分段扩增的策略对ClYVV全基因组序列进行克隆，序列测定后拼接获得江苏蚕豆三叶草黄脉病毒（ClYVV-JS）全基因组序列。基因组序列分析结果显示该分离物基因组序列全长9 585 bp，编码11个蛋白质。NCBI序列比对结果表明该分离物与已报道的日本ClYVV No.30分离物（AB011819）核苷酸序列同源性最高，达96.37%，其中编码的氨基酸序列同源性高达99.06%，而与中国合肥ClYVV分离物（KU922565）核苷酸序列同源性相对较低（94.74%）。聚类分析结果也显示ClYVV-JS归入三叶草黄脉病毒分支，与马铃薯Y病毒属其他成员分别归入不同分支。
Abstract:
Clover yellow vein virus (ClYVV), belonging to the genus Potyvirus, infects a variety of legume crops and causes serious damage. In order to clarify the whole genomic sequences and structure characteristics of ClYVV detected in the broad samples collected from Yancheng, Jiangsu province, the genomic fragments were cloned and sequenced. Sequenced fragments were assembled to obtain the full-length sequence of ClYVV-JS. Genomic sequence analysis showed that the genome sequence of isolate was 9 585 bp in length, encoding 11 proteins. Sequence alignment results showed that ClYVV-JS shared the highest nucleotide sequence identity (96.37%) with a Japanese ClYVV No.30 isolate (AB011819), and the amino acid sequence identity was 99.06%. While ClYVV-JS shared relative low nucleotide sequence identity (94.74%) with the Chinese Hefei ClYVV isolate (KU922565) reported earlier. The results of cluster analysis also indicated that ClYVV-JS was clustered in the same clade with other ClYVV isolates, while different clade with other members of the genus Potyvirus.

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备注/Memo

备注/Memo:
收稿日期：2022-07-11基金项目：江苏省农业科技自主创新基金项目[CX（21）1011]；国家自然科学基金项目（32072506）；现代农业产业技术体系建设专项（CARS-24-C-01）作者简介：高晓晓（1998-），女，河南濮阳人，硕士研究生，主要从事分子植物病毒学研究。（E-mail）2424054181@qq.com通讯作者：章松柏，（E-mail）yangtze2008@126.com；季英华，（E-mail）jiyinghua@jaas.ac.cn
更新日期/Last Update: 2022-11-07