[1]蒋正宁,赵仁慧,陈甜甜,等.分子标记辅助选育兼抗赤霉病、白粉病和黄花叶病毒病的小麦新品系[J].江苏农业学报,2021,(05):1100-1107.[doi:doi:10.3969/j.issn.1000-4440.2021.05.002]
　JIANG Zheng-ning,ZHAO Ren-hui,CHEN Tian-tian,et al.Molecular marker-assisted breeding of new wheat lines with resistances to Fusarium head blight, powdery mildew and wheat yellow mosaic virus[J].,2021,(05):1100-1107.[doi:doi:10.3969/j.issn.1000-4440.2021.05.002]
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分子标记辅助选育兼抗赤霉病、白粉病和黄花叶病毒病的小麦新品系()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年05期
页码:
1100-1107
栏目:
遗传育种·生理生化
出版日期:
2021-10-30

文章信息/Info

Title:
Molecular marker-assisted breeding of new wheat lines with resistances to Fusarium head blight, powdery mildew and wheat yellow mosaic virus
作者:
蒋正宁; 赵仁慧; 陈甜甜; 王玲; 吕国锋; 臧淑江; 别同德
（江苏里下河地区农业科学研究所/农业部长江中下游小麦生物学与遗传育种重点实验室，江苏扬州225007）
Author(s):
JIANG Zheng-ning; ZHAO Ren-hui; CHEN Tian-tian; WANG Ling; LYU Guo-feng; ZANG Shu-jiang; BIE Tong-de
(Key Laboratory of Wheat Biology and Genetic Improvement in Low ＆ Middle Yangtze River Valley Winter Wheat Region/Institute of Agricultural Sciences of the Lixiahe District in Jiangsu Province, Yangzhou 225007, China)
关键词:
小麦; 抗病基因; 聚合育种; 赤霉病; 白粉病; 黄花叶病毒病
Keywords:
wheat; disease resistance gene; pyramiding breeding; Fusarium head blight; powdery mildew; wheat yellow mosaic virus
分类号:
S512.1;Q343.2+45
DOI:
doi:10.3969/j.issn.1000-4440.2021.05.002
文献标志码:
A
摘要:
小麦赤霉病、白粉病和黄花叶病毒病是长江中下游麦区的主要病害，因此培育多抗品种是小麦绿色生产的基础。扬麦18是多抗高产小麦品种，具有抗赤霉病基因Fhb1、抗白粉病基因Pm21和抗黄花叶病毒病数量性状位点（QTL）QYm.njau-5A，其不足是株高较高、抗倒性差。扬麦22是抗白粉病的高产品种，携带抗白粉病基因PmV，但中感赤霉病，高感黄花叶病毒病。为了培育矮秆抗倒、兼抗3种病害的小麦新品系，以扬麦18/扬麦22重组自交系群体（RIL）为材料，研究Fhb1、QYm.njau-5A在群体中的分布及其对株高、粒质量的遗传效应，并对多基因聚合效率进行评估。结果表明，在RIL群体中，Fhb1、QYm.njau-5A的分布频率分别为49.0%、50.5%，符合Hardy-Weinberg定律，说明二者可在亲子代自由传递；Fhb1阳性RIL组群的平均病小穗数显著低于Fhb1阴性RIL组群，Pm21、PmV阳性RIL家系均表现为高抗白粉病；单个QYm.njau-5A能够满足抗小麦黄花叶病毒病的育种需求；Fhb1对株高、粒质量没有显著影响；QYm.njau-5A对株高有显著的增效作用，对粒质量没有显著影响；育成兼抗3种病害的矮秆高产新品系（组合）扬17J103、扬杂麦1号进入区域试验。研究结果为利用分子标记辅助选择技术培育矮秆多抗小麦新品种提供了优异的育种材料和理论依据。
Abstract:
Fusarium head blight, powdery mildew and wheat yellow mosaic virus are three major wheat diseases in the middle and lower reaches of Yangtze River. Breeding of multiple-resistant wheat varieties is the basis of green production. Yangmai 18 is a high-yield variety carrying Fhb1, Pm21 and QYm.njau-5A, leading to the resistances to Fusarium head blight, powdery mildew and yellow mosaic virus, respectively, but it has poor lodging resistance. Yangmai 22 carrying PmV is also a high-yield and powdery mildew resistant variety, but moderately susceptible to Fusarium head blight and highly susceptible to yellow mosaic virus. To breed new wheat lines with resistance to lodging and three diseases, the distribution of Fhb1 and QYm.njau-5A in Yangmai 18/Yangmai 22 recombinant inbred line (RIL) population and their genetic effects on plant height and grain weight were studied, and the efficiency of polygene polymerization was evaluated. The results showed that distribution frequencies of Fhb1 and QYm.njau-5A in RIL population were 49.0% and 50.5%, respectively, which accorded with Hardy-Weinberg law, indicating that both the genes could be freely transmitted from parent to offspring. The average number of diseased spikelets in Fhb1 positive RIL group was significantly lower than that in Fhb1 negative RIL group. All the RIL families carrying Pm21 or PmV showed high resistance to powdery mildew. A single QYm.njau-5A could meet the breeding needs of wheat lines with resistance to yellow mosaic virus. Fhb1 had no significant effect on plant height and kernel weight. Qym.njau-5A had significant synergistic effect on plant height, but had no significant effect on kernel weight. The dwarf and high-yield lines Yang 17J103 and Yangzamai 1 with resistance to three diseases were bred. These results provide excellent breeding materials and theoretical basis for breeding dwarf and multi-resistance wheat varieties by molecular marker-assisted selection.

参考文献/References:

[1]LI G Q, ZHU J Y, JIA H Y, et al. Mutation of a histidine-rich calcium-binding-protein gene in wheat confers resistance to Fusarium head blight[J]. Nature Genetics, 2019,51(7):1106-1112.
[2]SU Z Q, BERNARDO A, TIAN B, et al. A deletion mutation in TaHRC confers Fhb1 resistance to Fusarium head blight in wheat[J]. Nature Genetics, 2019,51(7):1099-1105.
[3]朱展望,徐登安,程顺和,等.中国小麦品种抗赤霉病基因Fhb1的鉴定与溯源[J].作物学报, 2018, 44(4): 473-482.
[4]ZHU X B, WANG H Y, GUO J, et al．Mapping and validation of quantitative trait loci associated with wheat yellow mosaic bymo-virus resistance in bread wheat[J]. Theroretical and Applied Genetics, 2012, 124(1): 177-188.
[5]CHEN P D, QI L L, ZHOU B, et al. Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew[J]. Theroretical and Applied Genetics,1995,91(6/7):1125-1128.
[6]别同德,高德荣,张晓,等. 基于高代姊妹系组群研究小麦-簇毛麦染色体T6VS.6AL易位的遗传效应[J]．江苏农业学报，2015, 31(6):1206-1210.
[7]LI H B, CHEN X, XIN Z Y, et al. Development and identification of wheat-Haynaldia villosa T6DL.6VS chromosome translocation lines conferring resistance to powdery mildew[J]. Plant Breeding, 2008, 124(2):203-205.
[8]BIE T D，ZHAO R H，ZHU S Y，et al. Development and characterization of maker MBH1 simultaneously tagging genes Pm21 and PmV conferring resistance to powdery mildew in wheat[J]. Molecular Breeding, 2015, 35:189.
[9]ZHAO R H, LIU B L, JIANG Z N, et al. Comparative analysis of genetic effects of wheat-Dasypyrum villosum translocations T6V#2S·6AL and T6V#4S·6DL[J]. Plant Breeding, 2019, 138(5):503-512.
[10]张伯桥. 应用滚动回交选育抗白粉病小麦新品种扬麦18[J].中国农学通报, 2009, 25(13): 74-77.
[11]吴宏亚,张伯桥,高德荣,等. 优质弱筋抗白粉病小麦新品种扬22[J]. 麦类作物学报, 2014, 34(6):876.
[12]TAPLA-TUSSELL R,QUIJANO-RAMAYO A, ROJAS-HERREAR R, et al. A fast, simple, and reliable high-yielding method for DNA extraction from different plant species[J]. Molecular Biotechnology, 2005, 31(2):137-139.
[13]ZHANG X H, PAN H Y, BAI G H, et al. Quantitative trait loci responsible for Fusarium head blight resistance in Chinese landrace Baishanyuehuang[J]. Theoreical and Applied Genetics, 2012, 125(3): 495-502.
[14]HE H G, ZHU S Y, JIANG Z N, et al. Comparative mapping of powdery mildew resistance gene Pm21 and functional characterization of resistance related genes in wheat[J]. Theroretical and Applied Genetics, 2016, 129(4): 819-829.
[15]刘伟华,何震天,耿波,等. 小麦对黄花叶病的抗性鉴定及典型品种的遗传分析[J].植物病理学报，2004，34(6):542-547．
[16]张旭,姜朋,叶人元,等. 宁麦9号及其衍生品种的赤霉病抗性分析及抗性溯源[J].分子植物育种, 2017,15(3):1053-1060.
[17]WANG H, SUN S, GE W, et al. Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat[J]. Science, 368(6493), eaba5435.
[18]赵仁慧,刘炳亮,寿路路,等. 分子标记辅助聚合抗小麦黄花叶病和白粉病育种[J]. 麦类作物学报, 2017, 37(12):1541-1549
[19]李生荣,陶军,杜小英,等. 强优势小麦杂交种‘绵杂麦168’选育研究[J].农学学报, 2011,1(11): 26-29.

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备注/Memo

备注/Memo:
收稿日期：2021-02-19基金项目：国家重点研发计划项目（2016YFD0101601）; 国家自然科学基金项目（32072065）作者简介：蒋正宁(1979-)，男，江西景德镇人，博士，副研究员，主要从事小麦遗传育种研究。（E-mail）13665219751@163.com通讯作者：别同德,(E-mail)bietd@126.com
更新日期/Last Update: 2021-11-09