[1]陈蓉,应诗家,陈哲,等.AMH及其相关基因在鸭不同等级卵泡中的表达[J].江苏农业学报,2018,(01):87-92.[doi:doi:10.3969/j.issn.1000-4440.2018.01.013]
　CHEN Rong,YING Shi-jia,CHEN Zhe,et al.Expression of AMH and its related genes in various-sized follicles of duck[J].,2018,(01):87-92.[doi:doi:10.3969/j.issn.1000-4440.2018.01.013]
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AMH及其相关基因在鸭不同等级卵泡中的表达()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年01期
页码:
87-92
栏目:
畜牧兽医·水产养殖
出版日期:
2018-02-25

文章信息/Info

Title:
Expression of AMH and its related genes in various-sized follicles of duck
作者:
陈蓉; 应诗家; 陈哲; 于建宁; 施振旦
（江苏省农业科学院畜牧研究所，江苏南京210014）
Author(s):
CHEN Rong; YING Shi-jia; CHEN Zhe; YU Jian-ning; SHI Zhen-dan
（Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjng 210014, China）
关键词:
抗缪勒氏管激素; 鸭; 卵泡; 基因表达
Keywords:
AMH; duck; follicles; gene expression
分类号:
S858.32
DOI:
doi:10.3969/j.issn.1000-4440.2018.01.013
文献标志码:
A
摘要:
本研究旨在分析AMH及其相关基因在鸭不同等级卵泡中的表达特征，为水禽卵泡发育的分子机制研究积累基础资料。以连城白鸭为研究对象，通过定量PCR技术分别检测了卵巢不同等级卵泡中AMH基因及其相关调控基因的表达情况。结果表明，随着卵泡的发育，AMH及其相关基因AMHR2、BMP6、SF1、GATA4和WT1的表达量逐渐降低，而CYP19A1基因的表达量逐渐升高，SOX9和FSHR基因的表达量保持不变。我们推测AMH基因在鸭等级前卵泡中的高表达量可能有助于维持颗粒细胞未分化的状态，且可能是通过抑制FSHR信号通路来实现的。
Abstract:
To investigate the expression of AMH and its related genes in various-sized follicles of duck and provide the basic data for the molecular mechanism of waterfowl follicle development, the expression levels of AMH gene and its related regulatory genes in ovarian various-sized follicles from Liancheng duck were determined by quantitative PCR in this study. The results showed that the expression levels of AMH and its related genes AMHR2, BMP6, SF1, GATA4 and WT1 decreased gradually with the development of follicles, while the expression level of CYP19A1 gene increased gradually and the expression levels of SOX9 and FSHR genes remained unchanged. We speculated that the high expression of AMH gene in prehierarchal follicles of duck was helpful to maintain the undifferentiated state of granulosa cells, which might be achieved by inhibiting FSHR signaling pathway.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2017-07-10 基金项目：江苏省自然科学基金项目（BK20160592）；江苏省农业科技自主创新基金项目[CX(14)5032] 作者简介：陈蓉（1985-），女，江苏淮安人，博士，助理研究员，主要从事家禽遗传育种与繁殖方面的研究。（E-mail）chenrong_big@163.com 通讯作者：施振旦，（E-mail）zdshi@jaas.ac.cn
更新日期/Last Update: 2018-03-06