[1]刘子记,申龙斌,朱婕,等.苦瓜α-苦瓜素基因克隆与单倍型分析[J].江苏农业学报,2017,(05):1117-1123.[doi:doi:10.3969/j.issn.1000-4440.2017.05.024]
　LIU Zi-ji,SHEN Long-bin,ZHU Jie,et al.The cloning and haplotype analysis of α-momorcharin from bitter gourd[J].,2017,(05):1117-1123.[doi:doi:10.3969/j.issn.1000-4440.2017.05.024]
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苦瓜α-苦瓜素基因克隆与单倍型分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年05期
页码:
1117-1123
栏目:
园艺
出版日期:
2017-10-30

文章信息/Info

Title:
The cloning and haplotype analysis of α-momorcharin from bitter gourd
作者:
刘子记1; 申龙斌1; 朱婕2; 牛玉1; 杨衍1
(1.中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点开放实验室，海南儋州571737；2. 海南大学热带农林学院，海南海口570228)
Author(s):
LIU Zi-ji1; SHEN Long-bin1; ZHU Jie2; NIU Yu1; YANG Yan1
(1.Tropical Crops Genetic Resources Institute，Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China，Ministry of Agriculture， Danzhou 571737，China；2.Institute of Tropical Agriculture and Forestry，Hainan University， Haikou 570228，China)
关键词:
苦瓜; α-苦瓜素基因; 克隆; 单倍型
Keywords:
bitter gourd; α-momorcharin gene; cloning; haplotype
分类号:
S642.5
DOI:
doi:10.3969/j.issn.1000-4440.2017.05.024
文献标志码:
A
摘要:
为了揭示苦瓜α-苦瓜素基因的完整结构及单倍型，本研究以35份苦瓜初级核心种质为材料，克隆α-苦瓜素基因，结果显示，α-苦瓜素基因全长993 bp，包括60 bp的5′-UTR，72 bp的3′-UTR，861 bp的ORF，无内含子序列，编码286个氨基酸，α-苦瓜素蛋白含有RIP蛋白保守结构域。系统进化分析结果表明，除苦瓜RIP P16094.2外，α-苦瓜素蛋白与胶苦瓜3MRW_A的亲缘关系最近，而与苦瓜MAP30蛋白的亲缘关系较远。通过比较35份苦瓜种质资源α-苦瓜素基因序列，共发现5个SNP位点，5个SNP位点均位于编码区。SNP分组发现，35份苦瓜种质资源α-苦瓜素基因共存在5种单倍型。5种单倍型编码的氨基酸序列比对显示，共存在1个氨基酸变异位点，氨基酸变异位点对应于第5个SNP位点，第1、第2、第3、第4 SNP位点并未引起氨基酸的改变。该结果为进一步研究α-苦瓜素基因表达调控机制和不同单倍型编码蛋白功能差异奠定了基础。
Abstract:
In order to reveal the whole gene structure and haplotype of α-momorcharin, 35 primary core germplasm of bitter gourd were used as materials, and the α-momorcharin gene was cloned. The results showed that α-momorcharin gene was 993 bp in length, including 60 bp 5′-UTR, 72 bp 3′-UTR, and 861 bp ORF, with no intron, encoding 286 amino acids. α-momorcharin protein contained a conserved domain of RIP protein. Phylogenetic analysis showed that in addition to P16094.2, the relationship between α-momorcharin and 3MRW_A of Momordica balsamina was closest, but the relationship between than α-momorcharin and MAP30 was far. Five SNP loci were found by comparing the α-momorcharin gene sequences of 35 bitter gourd germplasm. All of the five SNP loci were located in the coding region. Five haplotypes were found through SNP grouping in 35 bitter gourd germplasm. One amino acid mutation was found according to comparing the amino acid sequences encoded by the five haplotypes, which was corresponding to the 5th SNP loci, the 1st, 2nd, 3rd and 4th SNP loci did not cause amino acid changes. The results provides a basis for further study on the regulation mechanism of α-momorcharin gene expression and the function difference of proteins encoded by different haplotypes.

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备注/Memo

备注/Memo:
收稿日期：2017-05-09 基金项目：中央级公益性科研院所基本科研业务费专项（1630032014019、1630032015015、1630032016019）;国家自然科学基金项目（31601758） 作者简介：刘子记（1982-），男，山东菏泽人，博士，副研究员，研究方向：蔬菜分子生物学及遗传育种。（E-mail） liuziji1982@163.com 通讯作者：杨衍，（E-mail）catasvegetable@126.com
更新日期/Last Update: 2017-11-03