[1]吴立东,林淑婷,刘亚婷,等.基于SRAP标记和主要表型性状的苦瓜优株选择与遗传分析[J].江苏农业学报,2019,(05):1175-1183.[doi:doi:10.3969/j.issn.1000-4440.2019.05.025]
　WU Li-dong,LIN Shu-ting,LIU Ya-ting,et al.Genetic analysis and superior plant selection of bitter gourd based on SRAP markers and main phenotypic traits[J].,2019,(05):1175-1183.[doi:doi:10.3969/j.issn.1000-4440.2019.05.025]
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基于SRAP标记和主要表型性状的苦瓜优株选择与遗传分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年05期
页码:
1175-1183
栏目:
园艺
出版日期:
2019-10-31

文章信息/Info

Title:
Genetic analysis and superior plant selection of bitter gourd based on SRAP markers and main phenotypic traits
作者:
吴立东; 林淑婷; 刘亚婷; 钟金仙; 罗英
(三明市农业科学研究院，福建沙县365509)
Author(s):
WU Li-dong; LIN Shu-ting; LIU Ya-ting; ZHONG Jin-xian; LUO Ying
(Sanming Academy of Agricultural Sciences， Shaxian 365509， China)
关键词:
苦瓜; SRAP标记; 主成分分析; 优株选择
Keywords:
bitter gourd; SRAP markers; principal component analysis; superior plant selection
分类号:
S642.5
DOI:
doi:10.3969/j.issn.1000-4440.2019.05.025
文献标志码:
A
摘要:
为了培育出高产、优质的苦瓜新品种，选择48份苦瓜自交系，测定其10个数量性状和2个质量性状，采用主成分分析法进行优株选择，同时结合SRAP分子标记技术进行遗传分析。结果表明，10个数量性状的累积方差贡献率达68.897%，提取了反映苦瓜10个数量性状的4个主成分；通过计算10个数量性状的综合评价值，在48份苦瓜供试材料中，选出3个综合性状优良的材料，其结果与实际田间表现相近；结合苦瓜的2个质量性状，发现条型瓜瘤与苦瓜果实产量呈正相关，瓜横径与苦瓜种子产量呈正相关；结合SRAP分子聚类与主要表型性状特征，发现控制瓜瘤类型的基因并非简单的调控，条型瓜瘤控制基因与绿色瓜皮色控制基因、粒型瓜瘤控制基因与白色瓜皮色控制基因存在某种程度的连锁。这为苦瓜亲本选配和良种选育提供了理论依据。
Abstract:
In order to breed new bitter gourd varieties with high yield and high quality, 48 inbred lines of bitter gourd were selected to determine 10 quantitative characters and two qualitative traits. The principal component analysis was used to select the superior plants, and the SRAP molecular marker technology was used to analyze the genetic characteristics. The results showed that the cumulative variance contribution rate of the 10 quantitative characters reached 68.897％, four principal components reflecting 10 quantitative traits of bitter gourd were extracted. By calculating the comprehensive evaluation value of the 10 quantitative characters, three materials with excellent comprehensive characters were selected in the 48 bitter gourd test materials, and the results were similar to the field performance. Combined with the two qualitative traits of bitter gourd, it was found that there was a positive correlation between the fruit yield of bitter gourd and the bar-shaped bitter gourd lump, and there was also a positive correlation between the horizon diameter of bitter gourd and seed yield of bitter gourd. Combining SRAP molecular clustering with main phenotypic traits, it was found that the gene controlling the tumor type of bitter gourd was not simply regulated, and there was a certain degree of linkage between the control gene of the stripe bitter gourd tumor and the control gene of the green bitter gourd skin color, the control gene of the grain bitter gourd tumor and the control gene of the white bitter gourd skin color. This provides a theoretical basis for parent selection and seed selection of bitter gourd

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备注/Memo

备注/Memo:
收稿日期：2019-01-08 基金项目：三明市科技计划项目(2019-N-3)；三明市农业科学研究院院立项目(2016-Y-1) 作者简介：吴立东(1981-)，男，福建省宁化人，硕士，副研究员，主要研究方向为蔬菜种质资源与遗传育种。(E-mail)smnkswld@163.com。林淑婷为共同第一作者。 通讯作者：罗英，(E-mail)54784027@qq.com
更新日期/Last Update: 2019-11-11