[1]张帅,齐颖颖,张红星,等.快速检测生鲜肉中三种食源性致病菌[J].江苏农业学报,2016,(04):939-945.[doi:10.3969/j.issn.100-4440.2016.04.036]
　ZHANG Shuai,QI Ying-ying,ZHANG Hong-xing,et al.Rapid detection of three foodborne pathogens in raw meat[J].,2016,(04):939-945.[doi:10.3969/j.issn.100-4440.2016.04.036]
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快速检测生鲜肉中三种食源性致病菌()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年04期
页码:
939-945
栏目:
加工贮藏·质量安全
出版日期:
2016-10-31

文章信息/Info

Title:
Rapid detection of three foodborne pathogens in raw meat
作者:
张帅; 齐颖颖; 张红星; 王怡雯; 谢远红; 刘慧
北京农学院食品科学与工程学院/食品质量与安全北京实验室/农产品有害微生物及农残安全检测与控制北京市重点实验室/北京市食品安全免疫快速检测工程技术研究中心/微生态制剂关键技术开发北京市工程实验室，北京 102206
Author(s):
ZHANG Shuai; QI Ying-ying; ZHANG Hong-xing; WANG Yi-wen; XIE Yuan-hong; LIU-Hui
Beijing Laboratory of Food Quality and Safety/Beijing Key Laboratory of Agricultural Product Detection and Control for Spoilage Organisms and Pesticide/Beijing Engineering Technology Research Center of Food Safety Immune Rapid Detection/Beijing Engineering Laboratory of Key Technology Development of Microecologics/College of Food Science and Engineering, Beijing University of Agriculture, Beijing 102206，China
关键词:
食源性致病菌; 单克隆抗体; 多克隆抗体; 胶体金免疫层析试纸条
Keywords:
foodborne pathogen;  monoclonal antibody;  polyclonal antibody;  immunochromatographic strip
分类号:
S851.347.02
DOI:
10.3969/j.issn.100-4440.2016.04.036
文献标志码:
A
摘要:
为同时快速检测生鲜肉中鼠伤寒沙门氏菌、单增李斯特菌和福氏志贺氏菌3种食源性致病菌，降低检测成本，制备了特异性单克隆抗体和多克隆抗体，研制出能够同时检测以上3种致病菌的胶体金免疫层析试纸条。结果表明，本试验成功筛选出3株稳定分泌抗体的杂交瘤细胞株2E3、2E7和2E8，3种单克隆抗体效价均在1.28×106以上，3种多克隆抗体效价均在2.00×106以上。制备的胶体金免疫层析试纸条对鼠伤寒沙门氏菌、单增李斯特菌和福氏志贺氏菌3种食源性致病菌的特异性良好，灵敏度均为1×104CFU/g。
Abstract:
To achieve the simutaneous detection of Salmonella typhimurium, Listeria monocytogenes and Shigella flexneri in raw meat, three hybridoma cell strains, 2E3, 2E7 and 2E8, stably secreting antibodies against above pathogens were screened. The titers of three monoclonal antibodies and three polyclonal antibodies were more than 1.28×106 and 2.00×106, respectively. The immunochromatographic strip developed could detect all three pathogens as low as 1×104 CFU/g. L. monocytogenes was detected by the strip in market sample which was free of other two pathogens.

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备注/Memo

备注/Memo:
收稿日期：2016-05-20基金项目：北京市属高等学校高层次人才引进与培养长城学者计划（CIT&TCD20140315）；北京农学院协同创新建设项目作者简介：张帅(1992-)，男，山东聊城人，硕士研究生，主要从事食品安全快速检测研究。 (E-mail)15701299950@163.com通讯作者：张红星，(E-mail)hxzhang511@163.com
更新日期/Last Update: 2016-11-01