[1]李清,王英,刘小莉,等.戊糖片球菌对Caco-2细胞的黏附及黏附素的鉴定[J].江苏农业学报,2016,(04):927-933.[doi:10.3969/j.issn.100-4440.2016.04.034]
　LI Qing,WANG Ying,LIU Xiao-li,et al.The adhesion of Pediococcus pentosaceus to Caco-2 cell and identification of the adhesin[J].,2016,(04):927-933.[doi:10.3969/j.issn.100-4440.2016.04.034]
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戊糖片球菌对Caco-2细胞的黏附及黏附素的鉴定()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年04期
页码:
927-933
栏目:
加工贮藏·质量安全
出版日期:
2016-10-31

文章信息/Info

Title:
The adhesion of Pediococcus pentosaceus to Caco-2 cell and identification of the adhesin
作者:
李清1; 2; 王英1; 刘小莉1; 周剑忠1
1. 江苏省农业科学院农产品加工研究所，江苏 南京 210014；2.许昌职业技术学院医疗卫生系，河南 许昌 461000
Author(s):
LI Qing1; 2; WANG Ying1; LIU Xiao-li1; ZHOU Jian-zhong1; 2
1.Institute of Agricultural Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2.Xuchang Vocational and Technical College, Xuchang 416000, China
关键词:
戊糖片球菌; 黏附; Caco-2细胞; MUC3基因; 肽聚糖
Keywords:
Pediococcus pentosaceus;  adhesion;  Caco-2 cell;  MUC3 gene;  peptidoglycan
分类号:
TS255.54
DOI:
10.3969/j.issn.100-4440.2016.04.034
文献标志码:
A
摘要:
为了研究戊糖片球菌SR2-6对Caco-2细胞的黏附作用，采用不同的化学试剂和酶处理SR2-6，测定其表面成分黏附性质变化，并提取戊糖片球菌表面的细胞黏附素，进行成分和结构分析。同时测量了Caco-2细胞MUC3基因表达量的变化。结果显示，戊糖片球菌SR2-6能明显上调Caco-2细胞MUC3基因表达；戊糖片球菌SR2-6的肽聚糖参与了Caco-2细胞黏附的主要过程，该肽聚糖为α型肽聚糖，提取物主要由谷氨酸、丙氨酸、天冬氨酸、赖氨酸组成，总糖含量为（9.42±1.55）%，蛋白质含量为 （4.61±1.88）%。戊糖片球菌SR2-6肽聚糖是一种抑制性黏附素，浓度越高，对SR2-6黏附Caco-2细胞的抑制越强，同时上调MUC3基因表达的作用也越强。
Abstract:
To study the adhesion of Pediococcus pentosaceus SR2-6 to Caco-2 cells, SR2-6 was treated differently with chemical agents and enzymes. The adhesin involved in the adhesion was extracted and the components and structures were analyzed. Peptidoglycan of SR2-6 exerted a major role in the adhesion processes. The peptidoglycan, demonstrated as the α-type, was a kind of inhibitory adhesin, consisting of glutamic acid, malonic acid, aspartic acid and lysine,with total sugar content of (9.42±1.55)% and protein content of (4.61±1.88)%. The higher the concentration of peptidoglycan, the stronger the inhibition against the adhesions of SR2-6 to Salmonella and Caco-2 cells, and the stronger the up-regulation of the MUC3 gene.

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备注/Memo

备注/Memo:
收稿日期：2015-11-09基金项目：江苏省农业科技自主创新项目[CX(13)3080]作者简介：李清（1993-），女，河南驻马店人，硕士，主要从事食品生物技术研究。（Tel）18768823906；（E-mail）elenaliqing@126.com
更新日期/Last Update: 2016-11-01