[1]杜改梅,吴结革,胡志华,等.断奶前后小鼠胃GOAT基因表达及其与胃酸分泌、胃泌素和生长抑素的关系[J].江苏农业学报,2016,(03):626.[doi:10.3969/j.issn.1000-4440.2016.03.022]
　DU Gai-mei,WU Jie-ge,HU Zhi-hua,et al.Expression of gastric GOAT mRNA and the relationship with gastric acid secretion, gastric gastrin and SS in pre- and post-weaning mice[J].,2016,(03):626.[doi:10.3969/j.issn.1000-4440.2016.03.022]
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断奶前后小鼠胃GOAT基因表达及其与胃酸分泌、胃泌素和生长抑素的关系()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年03期
页码:
626
栏目:
畜牧兽医·水产养殖
出版日期:
2016-06-30

文章信息/Info

Title:
Expression of gastric GOAT mRNA and the relationship with gastric acid secretion, gastric gastrin and SS in pre- and post-weaning mice
作者:
杜改梅1;  吴结革1;  胡志华1;  罗碧平1;  张玉红1;  韩正强1;  刘茂军2
1.金陵科技学院动物科学与技术学院,江苏 南京 210038; 2.江苏省农业科学院兽医研究所/农业部动物疫病诊断与免疫重点开放实验室/国家兽用生物制品工程技术研究中心,江苏 南京 210014
Author(s):
DU Gai-mei1;  WU Jie-ge1;  HU Zhi-hua1;  LUO Bi-ping1;  ZHANG Yu-hong1;  HAN Zheng-qiang1; LIU Mao-jun2
1.Department of Animal Science and Technology, Jinling Technology Institution, Nanjing 210038, China; 2.Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China
关键词:
生长素;  GOAT;  胃功能;  断奶前后;  小鼠
Keywords:
ghrelin;  ghrelin O-acyltrans ferase(GOAT);  gastric function;  pre- and post-weaning;  mouse
分类号:
S852.2
DOI:
10.3969/j.issn.1000-4440.2016.03.022
文献标志码:
A
摘要:
为阐明小鼠断奶前后胃组织中生长素(Ghrelin)和Ghrelin酰基转移酶(GOAT)基因表达和蛋白质分泌与胃功能发育之间的关系,选取新生BALB/c小鼠10窝,分别于14、21、28、35、42、49和56日龄时随机选取10只小鼠(每窝1只)处死,采集胃黏膜组织样和血液,定量分析基因表达、蛋白质水平和H+-K+-ATPase活性。结果表明,胃GOAT mRNA表达和蛋白质水平均受到断奶的影响,并首次发现断奶时胃GOAT基因表达和蛋白质水平显著下降,GOAT mRNA表达与胃H+-K+-ATPase活性成显著正相关。胃黏膜组织中生长抑素(SS)和胃泌素(Gastrin)基因mRNA表达在21、28和35日龄较低,随后逐渐增加,45日龄时显著升高(P<0.05)。断奶前后胃可能通过自身调节GOAT水平来适应饮食的变化,表明GOAT可能参与对胃功能发育,生长抑素可能对GOAT有抑制作用。
Abstract:
To investigate how the stomach modulates ghrelin production and secretion as well as ghrelin O-acyltransferase(GOAT)expression in pre- and post-weaning mice, the blood and gastric mucosal tissues of mice were collected for quantifying protein content and mRNA expression as well as H+-K+-ATPase. Newborn BALB/c mice were selected at 14 d, 21 d, 28 d, 35 d, 42 d, 49 d and 56 d of age respectively. The results revealed that weaning strongly regulated gastric GOAT mRNA expression and protein contents. For the first time, noticeable decreases were found in protein and mRNA levels of GOAT on weaning age, and GOAT mRNA expression was positively correlated with gastric H+-K+-ATPase activity. The mRNA expression levels of gastric somatostatin(SS)and gastrin rose from 21 d to 45 d of age, reaching a peak on 45 d of age(P<0.05). In conclusion, the stomach itself of pre- and post-weaning mice regulates ghrelin and GOAT production to adapt to diet change, and GOAT is involved in the gastric function development. SS down-regulates GOAT level.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-10-29
基金项目:国家自然科学基金项目(31302054); 江苏省自然科学基金项目(BK20131086); 江苏省农业科技自主创新基金项目[CX(13)3076]
作者简介::杜改梅(1976-),女,山西朔州人,博士,教授,主要从事营养和消化生理研究。
通讯作者:刘茂军,(E-mail)maojunliu@163.com
更新日期/Last Update: 2016-06-30