[1]李运生,童彬彬,刘晓蕊,等.生长素对猪孤雌激活胚胎体外发育的影响及其受体基因的表达模式[J].江苏农业学报,2015,(04):811-816.[doi:10.3969/j.issn.1000-4440.2015.04.016]
　LI Yun-sheng,TONG Bin-bin,LIU Xiao-rui,et al.In vitro development of porcine parthenogenetic embryos in response to ghrelin application and the expression of receptor gene GHSR-1a[J].,2015,(04):811-816.[doi:10.3969/j.issn.1000-4440.2015.04.016]
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生长素对猪孤雌激活胚胎体外发育的影响及其受体基因的表达模式()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年04期
页码:
811-816
栏目:
畜牧兽医·水产养殖
出版日期:
2015-08-31

文章信息/Info

Title:
In vitro development of porcine parthenogenetic embryos in response to ghrelin application and the expression of receptor gene GHSR-1a
作者:
李运生12; 童彬彬1; 刘晓蕊1; 凌英会12; 方富贵12; 张运海12; 刘亚12
（1.安徽农业大学动物科技学院，安徽合肥230036；2.安徽地方畜禽遗传资源保护与生物育种省级实验室，安徽合肥230036）
Author(s):
LI Yun-sheng12; TONG Bin-bin1; LIU Xiao-rui1; LING Ying-hui12; FANG Fu-gui12; ZHANG Yun-hai12; LIU Ya12
(1.College of Animal Science and Technology, Anhui Agricutural University，Heifei 230036, China;2.Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding， Heifei 230036, China)
关键词:
生长素; GHSR-1a; 猪孤雌激活胚
Keywords:
ghrelin; GHSR-1a; porcine parthenogenetic embryo
分类号:
S828
DOI:
10.3969/j.issn.1000-4440.2015.04.016
文献标志码:
A
摘要:
为探明生长素及其受体GHSR-1a在胚胎早期发育中的作用，以猪孤雌激活胚胎为研究对象，在其体外发育过程中添加不同浓度生长素，观察胚胎发育速度和发育效率，并用荧光定量PCR检测猪孤雌激活胚胎早期发育过程中生长素特异性受体基因GHSR-1a的表达。结果显示，相对于对照组，添加10.0 mg/L 生长素可以显著提高4-细胞胚胎、8-细胞胚胎、桑葚胚的发育率（P<0.05），并能加快4-细胞胚胎、8-细胞胚胎、桑葚胚的发育速度（P<0.05）。GHSR-1a mRNA在2-细胞期到桑葚胚期保持较高表达水平，囊胚期后显著下降（P<0.05）；免疫荧光染色表明GHSR-1a在猪孤雌激活胚胎各发育时期均有表达，4-细胞期、8-细胞期和桑葚胚期的表达量显著高于囊胚期（P<0.05）。推测生长素通过提高GHSR-1a的表达来提高猪孤雌激活胚胎的发育速度和效率。
Abstract:
The present study was designed to investigate the roles of exogenous ghrelin and its receptor GHSR-1a during early in vitro development of porcine parthenogenetic embryos. The developmental rates of 4-cell embryos, 8-cell embryos, and morula of porcine parthenogenetic embryos cultured in PZM3 supplemented with 10.0 mg/L ghrelin were significantly higher than control group(ghrelin free) (P<0.05), and the development of 4-cell embryos, 8-cell embryos, and morula were speeded up as well. The mRNA expression of GHSR-1a was identified at each stage of porcine parthenogenetic embryos （2-cell, 4-cell, 8-cell, morula and blastocyst）. Indirect immuno-fluorescence revealed that the protein GHSR-1a levels were higher at 4-cell stage, 8-cell stage and morula stage. It was suggested ghrelin improved the developmental rate and speed by increasing the receptor gene GHSR-1a expression.

参考文献/References:


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备注/Memo

备注/Memo:
收稿日期：2015-01-31 基金项目：高等学校博士科点专项科研基金博导类资助项目（20123418110004）；安徽省生猪产业技术体系项目（AHCYTX-06-03）；安徽农业大学学科骨干培育项目（2014XKPY-23） 作者简介：李运生（1980-），男，安徽凤台人，博士研究生，主要从事动物胚胎工程研究。(E-mail)lys4224@163.com 通讯作者：刘亚,(E-mail)liuya@ahau.edu.cn
更新日期/Last Update: 2015-08-31