[1]杨威,闫海霞,汪家璐,等.一株协助寄主植物缓解镉胁迫的芽孢杆菌筛选及其耐镉机制[J].江苏农业学报,2015,(04):806-810.[doi:10.3969/j.issn.1000-4440.2015.04.015]
　YANG Wei,YAN Hai-xia,WANG Jia-lu,et al.Selection of a Bacillus subtilis strain alleviating Cd2+ stress in host plants and the mechanism[J].,2015,(04):806-810.[doi:10.3969/j.issn.1000-4440.2015.04.015]
点击复制

一株协助寄主植物缓解镉胁迫的芽孢杆菌筛选及其耐镉机制()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年04期
页码:
806-810
栏目:
耕作栽培·资源环境
出版日期:
2015-08-31

文章信息/Info

Title:
Selection of a Bacillus subtilis strain alleviating Cd2+ stress in host plants and the mechanism
作者:
杨威1; 闫海霞2; 汪家璐1; 纪丽莲1; 王新风1
（1.淮阴师范学院生命科学学院/江苏省环洪泽湖生态农业生物技术重点实验室，江苏淮安223300；2.淮安市农业技术推广中心，江苏淮安223300）
Author(s):
YANG Wei1; YAN Hai-xia2; WANG Jia-lu1; JI Li-lian1; WANG Xin-feng1
(1.School of Life Sciences, Huaiyin Normal University/Jiangsu Key Laboratory for Eco-agricultural Biotechnology around Hongze Lake, Huaian 223300, China;2.Huaian Agro-tech Extension and Service Center, Huaian 223300, China)
关键词:
植物内生细菌; 镉胁迫; 枯草芽孢杆菌
Keywords:
plant endophytic bacterium; Cd stress; Bacillus subtilis
分类号:
S423.4
DOI:
10.3969/j.issn.1000-4440.2015.04.015
文献标志码:
A
摘要:
为研究植物内生细菌在缓解寄主植物镉污染中的作用，通过离体条件下耐镉活性鉴定，筛选了5株根围细菌和内生细菌，其中内生细菌菌株1JN2在以辣椒和黄瓜作为寄主进行的盆栽试验中能够增强寄主植物对镉的耐受性，与空白对照（不接种菌株、硫酸镉溶液培养）相比，黄瓜和辣椒叶绿素含量分别增加10.85%、20.14%，根系活力分别增加363.32%、525.51%，组织中镉离子浓度分别降低23.34%、40.39%，在5株细菌中对镉胁迫植物综合保护效果最佳。该菌株经形态学和分子学鉴定为枯草芽孢杆菌。扫描电镜检查发现，1JN2在镉离子处理后24 h开始进行菌体自我复原，在48 h出现胞外多糖固化现象。
Abstract:
Experiments on in vitro Cd tolerance were conducted on 183 bacteria strains, five of which were selected for their strong performance. Strain 1JN2 outplayed other four strains in improving host plants Cd-tolerance. Compared to blank control(bacterium free and Cd free), pot-cultured cucumber and pepper treated with strain 1JN2 under Cd stress showed greater chlorophyll contents by 10.85% and 20.14%, and stronger root vigor by 363.32% and 525.51%. The Cd concentrations in 1JN2-treated cucumber and pepper were decreased by 23.34% and 40.39%. Morphological and molecular identification revealed that 1JN2 was a strain of Bacillus subtilis. Scanning electron microscopy showed that strain 1JN2 started to heal itself 24 h post Cd stress and solidify extracellular polysaccharide 48 h post Cd stress.

参考文献/References:


[1]李秀兰,胡雪峰. 上海郊区蔬菜重金属污染现状及累积规律研究[J]. 化学工程师, 2005 (5): 36-38.
[2]张民,龚子同. 我国菜园土壤中某些重金属元素的含量与分布[J]. 土壤学报, 1996, 33(1): 85-93.
[3]NRIAGU J O, PACYNA J M. Quantitative assessment of world-wide contamination of air， water and soils by trace metals[J]. Nature, 1988, 333:134-139.
[4]余辉，张文斌，余建平. 洪泽湖表层沉积物重金属分布特征及其风险评价[J]. 环境科学, 2011, 32(2）：437-444.
[5]ZHUANG X L, CHEN J, SHIM H， et al. New advances in plant growth-promoting rhizobacteria for bioremediation[J]. Environment International, 2007, 33: 406-413.
[6]RATHNAYAKE I V N, MEGHARAJ M, BOLAN N， et al. Tolerance of heavy metals by gram positive soil bacteria[J]. World Academy of Science, Engineering and Technology, 2009, 53:1185-1190.
[7]GADD G M. Metals, minerals and microbes: geomicrobiology and bioremediation[J]. Microbiology, 2010, 156: 609-643.
[8]ZOUBOULIS A I, LOUKIDOU M X, MATIS K A. Biosorption of toxic metalsmith from aqueous solutions by bacteria strains isolated from metal-polluted soils[J]. Process Biochemistry, 2004, 39:909-916.
[9]魏本杰,曾晓希,刘志成,等. 产铁载体菌的筛选鉴定及活化镉的性能探究[J]. 环境科学与技术, 2014, 37(11): 26-31.
[10]周小梅,赵运林,胥正钢,等. 蒌蒿(Artemisia selengensis)根际耐镉产酸细菌的筛选鉴定及其生物学特性[J]. 环境工程学报, 2014, 8(11):5010-5014.
[11]曹喆,罗胜联,曾光明,等. 一株龙葵内生细菌 SDE06 去除 Cd2+ 的实验[J]. 微生物学通报, 2009, 36(3): 328-333.
[12]姜敏,曹理想,张仁铎. 重金属抗性内生真菌与其宿主植物重金属抗性关系初探[J]. 农业环境科学学报, 2008, 26(6): 2038-2042.
[13]张志良. 植物生理学实验指导[M]. 2版.北京: 高等教育出版社, 2002：59-62，88-91，257-258.
[14]邹文欣,谭仁祥. 植物内生菌研究新进展[J]. 植物学报, 2001(9):881-892.
[15]BERENDSEN R L, PIETERSE C M J， BAKKER P H M. The rhizosphere microbiome and plant health [J]. Trends in Plant Science, 2012, 17(8):478-486.
[16]宋小双,邓勋,遇文婧,等. 一株生防内生真菌D202对立枯丝核菌的抑制作用及生理学特性研究[J]. 安徽农业科学, 2014, 42(27):9274-9275.
[17]陈晓斌,张炳欣. 植物根围促生细菌(PGPR)作用机制的研究进展[J]. 微生物学杂志, 2000, 20(1):38-44.
[18]王娜,杨镇,马晓颖，等. 植物内生菌次生代谢产物对干旱胁迫下辣椒幼苗生理机制的影响[J]. 北方园艺, 2014(22):29-32.
[19]ZAHIR Z A, MUNIR A, ASGHAR H N， et al. Effectiveness of rhizobacteria containing ACC deaminase for growth promotion of peas (Pisum sativum) under drought conditions[J]. J Microbiol Biotechnol, 2008, 18(5)：958-963.
[20]EGAMBERDIEVA D, LUGTENBERG B. Use of plant growth-promoting rhizobacteria to alleviate salinity stress in plants[M]//Use of microbes for the alleviation of soil stresses. New York:Springer, 2014: 73-96.
[21]TEKAYA S B, TIPAYNO S, KIM K, et al. Rhizobacteria: restoration of heavy metal-contaminated soils[M]// AHMAD P, WANI M R. Physiological mechanisms and adaptation strategies in plants under changing environment. New York:Springer, 2014: 297-323.
[22]王银科,宋剑飞. 内生菌在修复重金属污染土壤的研究进展[J]. 北方环境, 2013(12):68-71.
[23]YANG W, XU Q, LIU H X， et al. Evaluation of biological control agents against Ralstonia wilt on ginger[J]. Biological Control, 2012, 62(3): 144-151.〖ZK）〗〖FL）〗

相似文献/References:

[1]王红,杨镇,裴文琪,等.功能性微生物制剂对镉胁迫下水稻生长及生理特性的影响[J].江苏农业学报,2016,(05):974.[doi:10.3969/j.issn.1000-4440.2016.05.003]
　WANG Hong,YANG Zhen,PEI Wen-qi,et al.Growth and physiological characteristics of cadmium-stressed rice influenced by functional microorganism agent[J].,2016,(04):974.[doi:10.3969/j.issn.1000-4440.2016.05.003]
[2]吴朝波,郭建春,符少萍,等.海雀稗对镉胁迫的生理响应及积累特性[J].江苏农业学报,2015,(06):1337.[doi:doi:10.3969/j.issn.1000-4440.2015.06.022]
　WU Chao-bo,GUO Jian-chun,FU Shao-ping,et al.Physiological responses of Paspalum vaginatum Sw. to Cd stress and its Cd accumulation[J].,2015,(04):1337.[doi:doi:10.3969/j.issn.1000-4440.2015.06.022]

备注/Memo

备注/Memo:
收稿日期：2014-12-06 基金项目：江苏省农业科技自主创新基金项目[CX（13）3026]；江苏省高校自然科学基金项目（14KJB210003） 作者简介：杨威（1983-)，男，河北承德人，博士，主要从事生物防治研究。（E-mail）yangw107@gmail.com 通讯作者：王新风，(Tel)0517-83526671;（E-mail）hywdwxf@sohu.com
更新日期/Last Update: 2015-08-31