[1]郭广君,孙茜,刘金兵,等.基于辣椒基因组重测序的InDel标记开发及应用[J].江苏农业学报,2015,(06):1400-1406.[doi:doi:10.3969/j.issn.1000-4440.2015.06.032]
　GUO Guang-jun,SUN Qian,LIU Jin-bing,et al.Development and application of pepper InDel markers based on genome re-sequencing[J].,2015,(06):1400-1406.[doi:doi:10.3969/j.issn.1000-4440.2015.06.032]
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基于辣椒基因组重测序的InDel标记开发及应用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年06期
页码:
1400-1406
栏目:
园　　艺
出版日期:
2015-12-31

文章信息/Info

Title:
Development and application of pepper InDel markers based on genome re-sequencing
作者:
郭广君; 孙茜; 刘金兵; 潘宝贵; 刁卫平; 戈伟; 高长洲; 王述彬
（江苏省农业科学院蔬菜研究所/江苏省高效园艺作物遗传改良重点实验室， 江苏南京210014）
Author(s):
GUO Guang-jun; SUN Qian; LIU Jin-bing; PAN Bao-gui; DIAO Wei-ping; GE Wei; GAO Chang-zhou; WANG Shu-bin
(Institute of Vegetables, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China)
关键词:
辣椒; 重测序; InDel标记; 种质鉴定
Keywords:
pepper; re-sequencing; InDel marker; germplasm identification
分类号:
S641.3
DOI:
doi:10.3969/j.issn.1000-4440.2015.06.032
文献标志码:
A
摘要:
基于全基因组重测序进行分子标记开发是一种新的方法和趋势。本研究基于辣椒全基因组重测序数据鉴定出的InDel位点，在2号染色体上随机选择并设计了40个InDel标记，以24份辣椒种质验证其有效性。结果显示：40个标记可获得扩增产物，其中具有多态性的标记35个，多态率为87.5%，同时35个标记可以有效地区分24份辣椒种质的多样性和特异性。以上结果表明，基于重测序数据开发辣椒InDel标记切实可行。
Abstract:
The development of molecular markers based on genome re-sequencing is a new method and trend. In this research, forty InDel loci on chromosome 2 of pepper were chosen randomly and designed by screening the InDel loci that had been identified based on pepper genome re-sequencing data. The effectiveness of InDel markers were tested on 24 pepper germplasm. Among the forty InDels, thirty-five were polymorphisc, accounting for 87.5% of the total, and could reveal the genetic diversity and specificity of the tested pepper germplasm, indicating that development of pepper InDel markers based on genome re-sequencing data was practicable. 

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备注/Memo

备注/Memo:
收稿日期：2015-07-22 基金项目：国家自然科学基金项目（31401866）；江苏省自然科学基金项目（4111415）；江苏省农业科技自主创新基金项目[CX（14）5013]；国家高技术研究发展计划（863计划）项目（2012AA100103）；现代农业产业技术体系建设专项基金项目（CARS-25） 作者简介：郭广君（1986-），女，山东聊城人，博士，助理研究员，研究方向为蔬菜遗传育种。(Tel)15996286331;（E-mail）ggj-198@163.com 通讯作者：王述彬，（E-mail）wangsbpep@vip.tom.com
更新日期/Last Update: 2015-12-31