[1]张朝阳,程瑞,徐兵划,等.BSA联合转录组分析发掘西瓜叶片黄化候选基因[J].江苏农业学报,2024,(01):165-173.[doi:doi:10.3969/j.issn.1000-4440.2024.01.018]
　ZHANG Chao-yang,CHENG Rui,XU Bing-hua,et al.Identification of candidate genes for watermelon leaf yellowing based on BSA and transcriptome analysis[J].,2024,(01):165-173.[doi:doi:10.3969/j.issn.1000-4440.2024.01.018]
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BSA联合转录组分析发掘西瓜叶片黄化候选基因()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年01期
页码:
165-173
栏目:
园艺
出版日期:
2024-01-30

文章信息/Info

Title:
Identification of candidate genes for watermelon leaf yellowing based on BSA and transcriptome analysis
作者:
张朝阳; 程瑞; 徐兵划; 顾妍; 黄大跃; 孙玉东
(江苏徐淮地区淮阴农业科学研究所/淮安市设施蔬菜重点实验室,江苏淮安223001)
Author(s):
ZHANG Chao-yang; CHENG Rui; XU Bing-hua; GU Yan; HUANG Da-yue; SUN Yu-dong
(Huaiyin Institute of Agricultural Sciences of the Xuhuai District of Jiangsu Province/Huaian Key Laboratory for Facility Vegetables, Huaian 223001, China)
关键词:
西瓜; 黄化; BSA; 遗传图谱; 基因定位
Keywords:
watermelon; etiolation; BSA; genetic map; gene location
分类号:
S651.01
DOI:
doi:10.3969/j.issn.1000-4440.2024.01.018
文献标志码:
A
摘要:
叶片是植物重要的功能器官之一,不仅是植株进行光合作用的主要场所,也可作为重要的形态标记,应用于育种中。叶片颜色作为形态标记,不仅可用于苗期杂种的清除,亦可用于种子纯度的测定。以西瓜全生育期叶片黄化突变体纯合自交系ly104为母本(P1)、绿叶自交系w3为父本(P2),通过杂交创制F1代、F2代、BC1代群体。遗传分析结果表明,该突变体的叶片黄化由单隐性基因控制。采用混合分组分析(BSA)进行初定位,通过简化基因组测序(RAD)开发全基因组单核苷酸多态性(SNP)标记构建西瓜高密度遗传图谱,将西瓜叶片黄化基因定位于2号染色体13 950 306～15 517 591 bp(大小约为1.57 Mb)。以西瓜97103v2为参考基因组,该区间包含24个注释基因。对P1(P1Y)、P2(P2G)和F2代群体中黄叶(F2Y)、绿叶(F2G)株系进行转录组水平分析,结果表明,目标区间内基因Cla97C02G035950、Cla97C02G036010、Cla97C02G036020、Cla97C02G036060在黄化叶片与正常绿叶材料中的表达量差异显著,可能是西瓜叶片的黄化候选基因。研究结果可为进一步解析西瓜叶片黄化基因功能和生物学特性奠定重要基础。
Abstract:
The leaf is one of the important functional organs of plants. It is not only the main place for photosynthesis of plants, but also can be used as an important morphological marker in breeding. As a morphological marker, leaf color can be used not only for removing hybrids at the seedling stage, but also for determining seed purity. In this study, F1, F2, and BC1 populations were created by hybridization, and the mutant homozygous inbred line ly104 in the whole growth period of watermelon was used as the female parent (P1), and the green leaf inbred line w3 was used as the male parent (P2). Genetic analysis showed that leaf yellowing was controlled by a single recessive gene. The bulked segregant analysis (BSA) was used for primary mapping, and genome-wide single nucleotide polymorphism (SNP) markers were developed by restriction-site associated DNA-sequencing (RAD) to construct a high-density genetic map of watermelon. The watermelon leaf yellowing gene was localized on chromosome 2 at 13 950 306-15 517 591 bp (about 1.57 Mb). Watermelon 97103v2 was used as the reference genome and the interval contained 24 annotated genes. The transcriptome levels of P1 (P1Y), P2 (P2G) and yellow leaf (F2Y) and green leaf (F2G) lines in F2 population were analyzed. The results showed that the expression levels of Cla97C02G035950, Cla97C02G036010, Cla97C02G036020 and Cla97C02G036060 in the target interval were significantly different between etiolated leaves and normal green leaves. These genes might be candidate genes for etiolation of watermelon leaves. The results of this study can lay an important foundation for further analysis of the function and biological characteristics of watermelon leaf yellowing genes.

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备注/Memo

备注/Memo:
收稿日期：2022-11-28基金项目：淮安市农业科学研究院发展基金项目(HAN201714)；淮安市自然科学研究技术专项(HAB202079)；国家西甜瓜产业技术体系淮安综合试验站项目(CARS-25)作者简介：张朝阳(1982-),男,江苏连云港人,硕士,副研究员,从事西甜瓜遗传育种研究。(E-mail)287362703@qq.com。程瑞为共同第一作者。通讯作者：孙玉东,(E-mail)sunyudong@jaas.ac.cn
更新日期/Last Update: 2024-03-17