[1]王继莲,王冬玲,周茜,等.盐碱地旱生芦苇根际解磷菌株筛选及促生特性[J].江苏农业学报,2024,(01):64-74.[doi:doi:10.3969/j.issn.1000-4440.2024.01.007]
　WANG Ji-lian,WANG Dong-ling,ZHOU Qian,et al.Screening and growth-promoting characteristics of phosphorus-solubilizing strains from the rhizosphere of Phragmites australis in saline-alkali soil[J].,2024,(01):64-74.[doi:doi:10.3969/j.issn.1000-4440.2024.01.007]
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盐碱地旱生芦苇根际解磷菌株筛选及促生特性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年01期
页码:
64-74
栏目:
耕作栽培·资源环境
出版日期:
2024-01-30

文章信息/Info

Title:
Screening and growth-promoting characteristics of phosphorus-solubilizing strains from the rhizosphere of Phragmites australis in saline-alkali soil
作者:
王继莲1; 2; 王冬玲1; 2; 周茜1; 2; 张甜1; 2; 李明源1; 2
(1.喀什大学生命与地理科学学院,新疆喀什844006；2.新疆帕米尔高原生物资源与生态自治区重点实验室,新疆喀什844006)
Author(s):
WANG Ji-lian1; 2; WANG Dong-ling1; 2; ZHOU Qian1; 2; ZHANG Tian1; 2; LI Ming-yuan1; 2
(1.The College of Life and Geographic Sciences, Kashi University, Kashi 844006, China;2.Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region, Kashi 844006, China)
关键词:
解磷菌; 盐碱土壤; 促生作用; 微生物肥料
Keywords:
phosphorus-solubilizing strains; saline-alkali soil; growth-promoting effects; microbial fertilizer
分类号:
Q939.96
DOI:
doi:10.3969/j.issn.1000-4440.2024.01.007
文献标志码:
A
摘要:
为进一步挖掘解磷菌菌种资源,研制适合于盐碱地作物的促生菌剂,利用选择性培养基从新疆克孜勒苏柯尔克孜自治州盐碱地旱生芦苇(Phragmites australis)根际土壤中筛选解磷菌株。结合限制性片段长度多态性技术与16S rDNA测序进行菌株鉴定,并挑选解磷能力突出的优良菌株开展植物接种试验。本试验共筛选到47株解磷菌,其中32株可溶解无机磷,溶磷量为55.8～722.3 mg/L；37株可溶解有机磷,溶磷量为11.8～34.4 mg/L。47株解磷菌隶属于7个属,以肠杆菌属(Enterobacter)占绝对优势。解磷菌株有效促进了拟南芥植株的生长和根系发育,以芽孢杆菌Bacillus sp.PN-2和类芽孢杆菌Paenibacillus sp.PM-1的促生作用最显著。解磷菌株对盐碱土中小麦地上部分(株高、茎粗、地上部分鲜质量和地上部分干质量)和地下部分(根鲜质量、根干质量)也表现出积极影响,以Enterobacter sp.PM-9和Enterobacter sp.PM-2促生作用最突出。本研究筛选的解磷菌株具有一定的应用潜力,对开发适用于盐碱地的微生物肥料有重要意义。
Abstract:
In order to further explore the resources of phosphorus-solubilizing bacteria and develop growth-promoting agents suitable for crops in saline-alkali land, phosphorus-solubilizing bacteria were screened from rhizosphere soil of dryland Phragmites australis in Xinjiang Kizilsu Kirgiz Autonomous saline-alkali soil. Strain identification was carried out by combining restriction fragment length polymorphism and 16S rDNA sequencing, and the strains with outstanding phosphorus-solubilizing ability were selected for plant inoculation test. As a result, a total of 47 strains of phosphate-solubilizing bacteria were screened. Among them, 32 strains could dissolve inorganic phosphorus with a phosphorus solubility of 55.8-722.3 mg/L. And 37 strains could dissolve organic phosphorus with a phosphorus solubility of 11.8-34.4 mg/L. The 47 phosphate-solubilizing bacteria belonged to seven genera, and Enterobacter was dominant. The phosphate-solubilizing strains could effectively promote the growth and root development of Arabidopsis thaliana, especially Bacillus sp.PN-2 and Paenibacillus sp.PM-1. The above-ground part (plant height, stem diameter, fresh weight and dry weight of above-ground part) and underground part (fresh weight and dry weight of root) of wheat in salin-alkali soil were also positively affected by the phosphate-solubilizing strain, Enterobacter sp.PM-9 and Enterobacter sp.PM-2 were the most prominent. The phosphate-solubilizing strains screened in this study have certain application potential and are of great significance for the development of microbial fertilizers suitable for saline-alkali soil.

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备注/Memo

备注/Memo:
收稿日期：2022-11-08基金项目：新疆维吾尔自治区自然科学基金项目(2021D01A18)作者简介：王继莲(1986-),女,山东菏泽人,硕士,副教授,研究方向为资源微生物。(E-mail)wjilian0710@sina.com通讯作者：李明源,(E-mail)mingyuan_lee@yeah.net
更新日期/Last Update: 2024-03-17