[1]刘芳,段盼盼,魏敏,等.辣椒CUL家族基因的鉴定与表达分析[J].江苏农业学报,2023,(06):1275-1285.[doi:doi:10.3969/j.issn.1000-4440.2023.06.002]
 LIU Fang,DUAN Pan-pan,WEI Min,et al.Identification and expression analysis of CUL gene family in pepper[J].,2023,(06):1275-1285.[doi:doi:10.3969/j.issn.1000-4440.2023.06.002]
点击复制

辣椒CUL家族基因的鉴定与表达分析()
分享到:

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年06期
页码:
1275-1285
栏目:
遗传育种·生理生化
出版日期:
2023-09-30

文章信息/Info

Title:
Identification and expression analysis of CUL gene family in pepper
作者:
刘芳段盼盼魏敏匡小妍马艳张涛马玉虎魏兵强
(甘肃农业大学园艺学院,甘肃兰州730070)
Author(s):
LIU FangDUAN Pan-panWEI MinKUANG Xiao-yanMA YanZHANG TaoMA Yu-huWEI Bing-qiang
(College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China)
关键词:
辣椒CUL基因家族生物信息学表达分析
Keywords:
pepperCULgene familybioinformaticsexpression analysis
分类号:
S641.3
DOI:
doi:10.3969/j.issn.1000-4440.2023.06.002
文献标志码:
A
摘要:
CUL家族成员在植物体内发挥着重要作用,但关于辣椒CUL基因家族的全基因组鉴定及其在非生物胁迫下的表达模式分析报道较少。本研究对辣椒CUL基因家族进行了全基因组鉴定和表达分析。结果表明,共有12个CaCUL家族基因被系统鉴定,编码序列(CDS)全长为777~2 952 bp,非均匀地分布在6条染色体上,系统进化树将CUL基因分为4个亚家族。保守结构域分析结果表明,所有CUL蛋白均含有Cullin结构域,除此之外,有8个成员含有Cullin-Nedd8结构域,CaCUL1.6蛋白含有NB-ARC结构域。亚细胞定位预测发现CaCUL均定位于细胞质、细胞核。CaCUL基因的启动子中有多种与激素和胁迫响应等相关的顺式作用元件。基因表达模式分析结果表明,在不同生长阶段的不同组织中CUL参与了辣椒植株的生长和发育;在非生物胁迫中,大部分CUL基因都具有应激反应。本研究为进一步解析CaCUL家族基因在辣椒生长发育和非生物胁迫应答中的作用机理奠定了基础。
Abstract:
CUL family members play an important role in plants, but there are few reports on the whole genome identification of CUL gene family and its expression pattern analysis under abiotic stress in pepper. In this study, the whole genome of CUL gene family in pepper was identified and its expression was analyzed. The results showed that, a total of 12 CaCUL family genes were systematically identified, and the total length of coding sequence (CDS) was 777-2 952 bp, which distributed on six chromosomes unevenly. CUL genes were divided into four subfamilies by phylogenetic tree. Analysis of the conserved domain showed that, all CUL genes contained Cullin domain. In addition, eight members contained Cullin-Nedd8 domain, and CaCUL1.6 contained NB-ARC domain. Prediction of subcellular localization showed that, CaCUL were located in cytoplasm and nucleus. There are many cis-acting elements related to hormones and stress response in the promoter of CaCUL gene. Results of gene expression pattern showed that, CUL participated in the growth and development of pepper plants in different tissues at different growth stages. In abiotic stresses, most CUL genes showed stress response. This study can lay foundation for further analysis of the mechanism of CaCUL family genes in pepper growth and abiotic stress response.

参考文献/References:

[1]CHHAPEKAR S, KEHIE M, RAMCHIARY N . Advances in molecular breeding of capsicum species[M]. New Delhi:Daya Publishing House, 2016:233-274.
[2]张帆. 辣椒Aux/IAA基因家族的鉴定与表达分析[D]. 长沙:湖南大学,2019.
[3]REGUERA M, PELEG Z, BLUMWALD E. Targeting metabolic pathways for genetic engineering abiotic stress-tolerance in crops[J]. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 2012, 1819(2): 186-194.
[4]GURUNG T, TECHAWONGSTIEN S, SURIHARN B, et al. Impact of environments on the accumulation of capsaicinoids in capsicum spp.[J]. Hort Science, 2011, 46(12): 1576-1581.
[5]KAOUTHER Z, MARIEM B F, FARDAOUS M, et al. Impact of salt stress (NaCl) on growth, chlorophyll content and fluorescence of Tunisian cultivars of chili pepper (Capsicum frutescens L.)[J]. Journal of Stress Physiology & Biochemistry, 2012, 8(4): 236-252.
[6]胡晨曦,李子恒,张云虹,等.低温弱光对不同品种辣椒幼苗生长和光合特性的影响[J]. 福建农业学报,2022,37(5):617-625.
[7]BASU S, RAMEGOWDA V, KUMAR A, et al. Plant adaptation to drought stress[J]. F1000Research, 2016, 5:1-10.
[8]郭卫丽. 辣椒对低温胁迫的响应与其低温抗性相关基因的克隆和功能分析[D]. 杨凌:西北农林科技大学,2013.
[9]ABOU-SREEA A I B, AZZAM C R, AL-TAWEEEL S K, et al. Natural biostimulant attenuates salinity stress effects in chili pepper by remodeling antioxidant, ion, and phytohormone balances, and augments gene expression[J]. Plants, 2021, 10(11): 2316.
[10]马潇. 辣椒CaCIPKs对干旱和低温胁迫的响应及其调控机理研究[D]. 杨凌:西北农林科技大学,2022.
[11]MOIN M, BAKSHI A, MADHAV M S, et al. Comprehensive expression profiling reveals the possible involvement of Cullins in developmental and stress regulation in rice[J]. Environmental and Experimental Botany, 2019, 160: 101-111.
[12]SHEN W H, PARMENTIER Y, HELLMANN H, et al. Null mutation of AtCUL1 causes arrest in early embryogenesis in Arabidopsis[J]. Molecular Biology of the Cell, 2002, 13(6): 1916-1928.
[13]REN C, PAN J, PENG W, et al. Point mutations in Arabidopsis Cullin1 reveal its essential role in jasmonate response[J]. The Plant Journal, 2005, 42(4): 514-524.
[14]PATTON E E, WILLEMS A R, SA D, et al. Cdc53 is a scaffold protein for multiple Cdc34/Skp1/F-box protein complexes that regulate cell division and methionine biosynthesis in yeast[J]. Genes & Development, 1998, 12(5): 692-705.
[15]KIPREOS E T, LANDER L E, WING J P, et al. cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family[J]. Cell, 1996, 85(6): 829-839.
[16]SARIKAS A, HARTMANN T, PAN Z Q. The cullin protein family[J]. Genome Biology, 2011, 12(4): 1-12.
[17]KUBO K, TSUKAHARA M, FUJII S, et al. Cullin1-P is an essential component of non-self recognition system in self-incompatibility in Petunia[J]. Plant and Cell Physiology, 2016, 57(11): 2403-2416.
[18]赵梦伊,魏春茹,范润侨,等. Cullin家族通过UPS途径响应小麦生长发育及非生物胁迫进程[J]. 分子植物育种,2021,20(21):1-17.
[19]MUELLER L A, SOLOW T H, TAYLOR N, et al. The SOL genomics network. A comparative resource for Solanaceae biology and beyond[J]. Plant Physiology, 2005, 138(3): 1310-1317.
[20]EL-GEBALI S, MISTRY J, BATEMAN A, et al. The Pfam protein families database in 2019[J]. Nucleic Acids Research, 2019, 47(1):427-432.
[21]LETUNIC I, DOERKS T, BORK P. SMART 7: recent updates to the protein domain annotation resource[J]. Nucleic Acids Research, 2012, 40(1):302-305.
[22]GASTEIGER E, HOOGLAND C, GATTIKER A, et al. Protein identification and analysis tools on the ExPASy server[J]. Humana Press, 2005: 571-607.
[23]CHOU K C, SHEN H B. A new method for predicting the subcellular localization of eukaryotic proteins with both single and multiple sites: Euk-mPLoc 2.0[J]. PLoS One, 2010, 5(4): e9931.
[24]ZHANG H X, CAO N, DONG C J, et al. Genome-wide identification and expression of ARF gene family during adventitious root development in hot pepper Capsicum annuum)[J]. Horticultural Plant Journal, 2017, 3(4): 151-164.
[25]WEI R M, XIE L L, OUYANG X, et al. Identification and expression analysis of ARF gene family in pepper[J]. Acta Botanica Boreali-Occidentalia Sinica, 2017, 37(6): 1047-1058.
[26]LIU F, YU H, DENG Y, et al. PepperHub, an informatics hub for the chili pepper research community[J]. Molecular Plant, 2017, 10(8): 1129-1132.
[27]杨佳惠,张冬冬,曾斌,等. 扁桃PsdCUL1基因克隆表达鉴定及其CUL基因家族分析[J/OL]. 分子植物育种,2022:1-23.https://kns.cnki.net/kcms/detail/46.1068.S.20220304.1133.006.html.
[28]PANG P X, SHI L, WANG X J, et al. Cloning and expression analysis of the StCUL1 gene in potato[J]. Journal of Plant Biochemistry and Biotechnology, 2019, 28(4): 460-469.
[29]HORI T, OSAKA F, CHIBA T, et al. Covalent modification of all members of human cullin family proteins by NEDD8[J]. Oncogene, 1999, 18(48): 6829-6834.
[30]宋刚,方志刚,王玉龙,等.柳枝稷BiP基因的鉴定、表达分析和抗逆境功能[J].江苏农业学报,2022,38(4):889-899.
[31]顾鹏鹏,马鑫磊,姚锐,等.谷子HSP90基因家族鉴定及干旱胁迫下表达分析[J].江苏农业科学,2022,50(6):45-52.
[32]HAN X M, CHEN Q X, YANG Q, et al. Genome-wide analysis of superoxide dismutase genes in Larix kaempferi[J]. Gene, 2019, 686: 29-36.
[33]丁祥青,胡敏杰,向双,等.秋茄WRKY基因家族的生物信息学分析[J].江苏农业科学,2022,50(14):50-60.
[34]沈丹,杨莉,胡威,等.柑橘胁迫响应基因WRKY47的克隆与表达分析[J].江苏农业学报,2021,37(1):129-138.
[35]JOUFFREY V, LEONARD A S, AHNERT S E. Gene duplication and subsequent diversification strongly affect phenotypic evolvability and robustness[J]. Royal Society Open Science, 2021, 8(6): 201636.
[36]KIM S H, WOO O G, JANG H, et al. Characterization and comparative expression analysis of CUL1 genes in rice[J]. Genes & Genomics, 2018, 40(3): 233-241.

相似文献/References:

[1]吕敏,苏建坤,白和盛,等.桃蚜取食和机械损伤对番茄和辣椒 PAL、LOX 和 PPO 活性的诱导作用[J].江苏农业学报,2016,(06):1273.[doi:doi:10.3969/j.issn.1000-4440.2016.06.013]
 Lv?Min,SU Jian-kun,BAI He-sheng,et al.The activities of PAL, LOX and PPO in tomato and pepper plants induced by aphid herbivory and mechanical damage[J].,2016,(06):1273.[doi:doi:10.3969/j.issn.1000-4440.2016.06.013]
[2]吴淑华,赵文浩,李廷芳,等.南京辣椒上一种斑驳类型病毒病的分子鉴定[J].江苏农业学报,2015,(06):1284.[doi:doi:10.3969/j.issn.1000-4440.2015.06.014]
 WU Shu-hua,ZHAO Wen-hao,LI Ting-fang,et al.Molecular identification of a virus causing mottle symptoms in pepper leaves in Nanjing[J].,2015,(06):1284.[doi:doi:10.3969/j.issn.1000-4440.2015.06.014]
[3]郭广君,孙茜,刘金兵,等.基于辣椒基因组重测序的InDel标记开发及应用[J].江苏农业学报,2015,(06):1400.[doi:doi:10.3969/j.issn.1000-4440.2015.06.032]
 GUO Guang-jun,SUN Qian,LIU Jin-bing,et al.Development and application of pepper InDel markers based on genome re-sequencing[J].,2015,(06):1400.[doi:doi:10.3969/j.issn.1000-4440.2015.06.032]
[4]李廷芳,吴淑华,赵文浩,等.青海海东设施辣椒轻斑驳病毒的分子检测[J].江苏农业学报,2017,(04):958.[doi:doi:10.3969/j.issn.1000-4440.2017.04.036]
 LI Ting-fang,WU Shu-hua,ZHAO Wen-hao,et al.Molecular detection of mild mottle virus isolated from pepper in Haidong, Qinghai province[J].,2017,(06):958.[doi:doi:10.3969/j.issn.1000-4440.2017.04.036]
[5]刘潮,韩利红,宋培兵,等.辣椒类甜蛋白基因家族鉴定及表达分析[J].江苏农业学报,2018,(01):122.[doi:doi:10.3969/j.issn.1000-4440.2018.01.018]
 LIU Chao,HAN Li-hong,SONG Pei-bing,et al.Identification and expression analysis of thaumatin-like protein gene in pepper[J].,2018,(06):122.[doi:doi:10.3969/j.issn.1000-4440.2018.01.018]
[6]王运儒,秦玉燕,杨秀娟,等.40%氯虫·噻虫嗪水分散粒剂在辣椒及土壤中的残留消解动态[J].江苏农业学报,2018,(01):207.[doi:doi:10.3969/j.issn.1000-4440.2018.01.030]
 WANG Yun-ru,QIN Yu-yan,YANG Xiu-juan,et al.Dissipation of chlorantraniliprole and thiamethoxam in pepper and soil after field application in the form of 40% water dispersible granules[J].,2018,(06):207.[doi:doi:10.3969/j.issn.1000-4440.2018.01.030]
[7]潘宝贵,钱恒彦,戈伟,等.辣椒应答冷信号转导机制研究进展[J].江苏农业学报,2019,(03):743.[doi:doi:10.3969/j.issn.1000-4440.2019.03.034]
 PAN Bao-gui,QIAN Heng-yan,GE Wei,et al.Research progress of cold signal transduction mechanisms in pepper[J].,2019,(06):743.[doi:doi:10.3969/j.issn.1000-4440.2019.03.034]
[8]高晶霞,吴雪梅,牛勇琴,等.辣根素水乳剂对连作辣椒生长及土壤酶活性的影响[J].江苏农业学报,2021,(01):116.[doi:doi:10.3969/j.issn.1000-4440.2021.01.015]
 GAO Jing-xia,WU Xue-mei,NIU Yong-qin,et al.Effect of athomin water emulsion on growth and soil enzyme activities of continuous cropping pepper[J].,2021,(06):116.[doi:doi:10.3969/j.issn.1000-4440.2021.01.015]
[9]郭广君,朱雪梅,潘宝贵,等.利用InDel分子标记辅助选育辣椒抗黄瓜花叶病毒病种质[J].江苏农业学报,2021,(05):1251.[doi:doi:10.3969/j.issn.1000-4440.2021.05.021]
 GUO Guang-jun,ZHU Xue-mei,PAN Bao-gui,et al.Innovation of pepper germplasm resource with resistance to cucumber mosaic virus by InDel molecular marker assisted selection[J].,2021,(06):1251.[doi:doi:10.3969/j.issn.1000-4440.2021.05.021]
[10]刘雪梅,王悦,李玮琦,等.辣椒miR169及其靶基因的生物信息学分析[J].江苏农业学报,2021,(06):1510.[doi:doi:10.3969/j.issn.1000-4440.2021.05.020]
 LIU Xue-mei,WANG Yue,LI Wei-qi,et al.Bioinformatics analysis of miR169 and its target genes in pepper[J].,2021,(06):1510.[doi:doi:10.3969/j.issn.1000-4440.2021.05.020]

备注/Memo

备注/Memo:
收稿日期:2022-11-01基金项目:兰州市人才项目(2021-RC-65);甘肃省重点研发项目(21YF5NA091)作者简介:刘芳(1995-),女,甘肃陇南人,硕士研究生, 主要从事蔬菜遗传育种与分子生物学研究。(E-mail)fliu2821@163.com通讯作者:魏兵强,(E-mail)bqwei@gsau.edu.cn
更新日期/Last Update: 2023-11-17