[1]龚镇洪,余彬彬,黄盼盼,等.罗红霉素对小麦硝态氮和铵态氮吸收动力学的影响[J].江苏农业学报,2022,38(04):882-888.[doi:doi:10.3969/j.issn.1000-4440.2022.04.003]
　GONG Zhen-hong,YU Bin-bin,HUANG Pan-pan,et al.Effects of roxithromycin on the absorption kinetics of nitrate nitrogen and ammonium nitrogen in wheat[J].,2022,38(04):882-888.[doi:doi:10.3969/j.issn.1000-4440.2022.04.003]
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罗红霉素对小麦硝态氮和铵态氮吸收动力学的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年04期
页码:
882-888
栏目:
遗传育种·生理生化
出版日期:
2022-08-31

文章信息/Info

Title:
Effects of roxithromycin on the absorption kinetics of nitrate nitrogen and ammonium nitrogen in wheat
作者:
龚镇洪1; 余彬彬1; 2; 3; 黄盼盼4; 汪晓丽1; 钱晓睛1
（1.扬州大学农业农村部耕地质量监测与评价重点实验室，江苏扬州225127；2.南开大学环境污染过程与基准教育部重点实验室，天津300071；3.农业部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室，天津300071；4.山东省东营市生态环境局，山东东营257100）
Author(s):
GONG Zhen-hong1; YU Bin-bin1; 2; 3; HUANG Pan-pan4; WANG Xiao-li1; QIAN Xiao-qing1
（1.Key Laboratory of Arable Land Quality Monitoring and Evaluation, Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225127, China;2.Key Laboratory of Pollution Processes and Environmental Criteria, Nankai University, Tianjin 300071, China;3.Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Agro-environment and Safe-product, Tianjin 300071, China;4.Dongying City Ecological Environment Bureau, Shandong, Dongying 257100, China）
关键词:
罗红霉素; 小麦; 硝态氮; 铵态氮; 吸收动力学
Keywords:
roxithromycin; wheat; nitrate nitrogen; ammonium nitrogen; absorption kinetics
分类号:
X592
DOI:
doi:10.3969/j.issn.1000-4440.2022.04.003
文献标志码:
A
摘要:
为明确在抗生素胁迫下小麦对不同形态无机氮的吸收特性，通过水培的方式，以硝态氮（NO-3-N）、铵态氮（NH+4-N）分别作为单一氮源，设置7个浓度水平的NO-3-N、NH+4-N和5个质量浓度水平的罗红霉素（Roxithromycin, ROX）组合，以不含ROX处理为对照（CK），探究在ROX胁迫下小麦根系对NO-3-N和NH+4-N的吸收动力学特征的变化。结果表明：（1）在不同质量浓度ROX胁迫下，小麦对NO-3-N、NH+4-N的最大吸收速率随氮浓度的增高而增加，吸收特征符合Michaelis-Menten动力学模型；与CK相比，不同质量浓度ROX胁迫对2种形态无机氮的最大吸收速率均表现为促进作用；（2）随着ROX胁迫质量浓度的增加，小麦对NO-3-N的最大吸收速率（Vmax）持续增加，Km值持续降低，吸收能力（α）持续增加；对NH+4-N的Vmax、Km值均持续增加，α值先增后降；（3）在10.0 mg/L ROX胁迫下，小麦对NO-3-N的吸收能力高于对NH+4-N的吸收能力，而在0.1 mg/L、0.5 mg/L和1.0 mg/L ROX胁迫下，小麦对NH+4-N的吸收能力超过对NO-3-N的吸收能力。
Abstract:
To clarify the absorption characteristics of different forms of inorganic nitrogen under antibiotic stress, hydroponics was used to cultivate wheats. Nitrate nitrogen （NO-3-N） and ammonium nitrogen （NH+4-N） were used as single nitrogen sources, respectively. Seven concentration levels of NO-3-N, NH+4-N and five concentration levels of roxithromycin (ROX) were established, and the treatment without ROX was used as control (CK). The absorption kinetics characteristics of NO-3-N and NH+4-N in wheat roots were explored under ROX stress. The results revealed that the maximum absorption rate of NO-3-N and NH+4-N in wheat increased with the increase of nitrogen concentration under different mass concentrations of ROX, and the absorption characteristics conformed to Michaelis-Menten equation. Compared with CK, different concentrations of ROX had a promoting effect on the maximum absorption rate of the two forms of inorganic nitrogen. With the increase of ROX concentration, the maximum adsorption rate (Vmax) and absorptive capacity (α)of wheat to NO-3-N increased continuously, and the Km value decreased continuously. In addition, the Vmax and Km values of wheat to NH+4-N increased continuously, and the α value increased firstly and then decreased. Under the stress of 10.0 mg/L ROX, the absorption capacity of wheat to NO-3-N was higher than that to NH+4-N. On the contrary, under the stress of 0.1 mg/L ROX, 0.5 mg/L ROX and 1.0 mg/L ROX, the absorption capacity of wheat to NH+4-N was higher than that to NO-3-N.

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备注/Memo

备注/Memo:
收稿日期：2021-10-23基金项目：国家自然科学基金项目(31500425)；江苏省自然科学基金青年科技人才项目(BK20150452)；农业部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室开放基金课题(16nybcdhj-4)；环境污染过程与基准教育部重点实验室(南开大学)开放基金课题 (KL-PPEC-2016-3)作者简介：龚镇洪（1998-），男，江苏常熟人，硕士研究生，主要从事植物生态毒理学研究。(E-mail)1441905782@qq.com通讯作者：余彬彬，(E-mail)bbyu@yzu.edu.cn
更新日期/Last Update: 2022-09-06