[1]赵娜,缪亚梅,姚梦楠,等.蚕豆种质资源籽粒表型与营养品质性状的多样性分析[J].江苏农业学报,2022,38(03):597-604.[doi:doi:10.3969/j.issn.1000-4440.2022.03.003]
　ZHAO Na,MIAO Ya-mei,YAO Meng-nan,et al.Diversity analysis on seed phenotypic and nutrient quality traits in faba bean germplasm resources[J].,2022,38(03):597-604.[doi:doi:10.3969/j.issn.1000-4440.2022.03.003]
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蚕豆种质资源籽粒表型与营养品质性状的多样性分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年03期
页码:
597-604
栏目:
遗传育种·生理生化
出版日期:
2022-06-30

文章信息/Info

Title:
Diversity analysis on seed phenotypic and nutrient quality traits in faba bean germplasm resources
作者:
赵娜; 缪亚梅; 姚梦楠; 薛冬; 顾春燕; 汪凯华; 王永强; 王学军
（江苏沿江地区农业科学研究所，江苏南通226541）
Author(s):
ZHAO Na; MIAO Ya-mei; YAO Meng-nan; XUE Dong; GU Chun-yan; WANG Kai-hua; WANG Yong-qiang; WANG Xue-jun
(Jiangsu Yanjiang Institute of Agricultural Sciences, Nantong 226541, China)
关键词:
蚕豆; 籽粒表型性状; 品质性状; 相关性分析; 主成分分析; 聚类分析
Keywords:
faba bean; seed phenotypic traits; quality traits; correlation analysis; principal component analysis; cluster analysis
分类号:
S529
DOI:
doi:10.3969/j.issn.1000-4440.2022.03.003
文献标志码:
A
摘要:
对蚕豆种质资源籽粒表型和营养品质性状的多样性进行评价，为中国蚕豆种质资源创新和品质育种提供参考。对55份蚕豆种质资源的6个籽粒表型性状和6个营养品质性状进行相关性分析、主成分分析和聚类分析。结果显示，籽粒表型性状变异系数为2.44%～32.94%，遗传多样性指数为1.861～2.046；营养品质性状变异系数为3.91%～44.22%，遗传多样性指数为1.174～2.038。相关性分析结果表明，籽粒营养品质性状与表型性状之间存在关联，蛋白质含量与粒宽、籽粒周长、籽粒表面积呈显著正相关，淀粉含量与粒长、粒宽、籽粒周长、籽粒表面积和百粒质量呈极显著负相关。前4个主成分的累计贡献率为78.152%，其中，第1主成分主要由籽粒表面积、粒长、籽粒周长、粒宽、百粒质量等性状决定，第2主成分主要由籽粒长宽比和单宁含量决定，第3主成分主要由淀粉含量和粗纤维含量决定，第4主成分主要由脂肪含量和蛋白质含量决定。通过聚类分析，将55份蚕豆资源分为5个类群，第Ⅰ类群为低淀粉含量、低脂肪含量、低纤维含量的中粒材料；第Ⅱ类群为高蛋白质含量、高脂肪含量的大粒优异材料；第Ⅲ类群为高单宁含量的大粒特异材料；第Ⅳ类群为高淀粉含量、高粗纤维含量、低单宁含量的中粒优异材料；第Ⅴ类群为低水分含量的小粒材料。
Abstract:
Genetic diversities of seed phenotypic traits and nutrient quality-related traits of faba bean germplasm resources were evaluated to provide reference for the innovation of faba bean germplasm resources and quality breeding in China. Correlation analysis, principal component analysis and cluster analysis of six seed phenotypic traits and six nutrient quality-related traits of 55 faba bean germplasm resources were conducted. The results demonstrated that, the variable coefficients of seed phenotypic traits were 2.44%-32.94%, and the genetic diversity indices were 1.861-2.046. The variable coefficients of nutrient quality-related traits were 3.91%-44.22%, and the genetic diversity indices were 1.174-2.038. Results of correlation analysis showed that, relevance was observed between seed nutrient quality-related phenotypic traits and phenotypic traits. Seed protein content was in significant positive correlation with seed width, seed perimeter and seed surface area, while starch content was in extremely significant negative correlation with seed length, seed width, seed perimeter, seed surface area and hundred-grain weight. Cumulative contribution rate of the front four main components was 78.152%. The first principal component was mainly determined by traits such as seed surface area, seed length, seed perimeter, seed width and hundred-seed weight. The second principal component was mainly determined by length-width ratio and tannin content of seed. The third principal component was mainly determined by starch content and crude fibre content. The fourth principal component was mainly determined by fat content and protein content. 55 faba bean resources were divided into five groups by cluster analysis. Among them, group Ⅰ belonged to moderate-sized materials with low contents of starch, fat and crude fibre. Group Ⅱ belonged to excellent large-sized materials with high contents of protein and fat. Group Ⅲ belonged to special large-sized materials with high tannin content. Group Ⅳ belonged to excellent middle-sized materials with high contents of starch and crude fibre, but with low content of tannin. Group Ⅴ belonged to small-sized materials with low moisture content.

参考文献/References:

[1]周俊玲，张蕙杰.世界蚕豆生产及贸易形势分析[J].世界农业，2016（11）：107-111.
[2]O′SULLIVAN D M, ANGRA D. Advances in faba bean genetics and genomics[J]. Frontiers in Genetics, 2016, 7. DOI: 10.3389/fgene.2016.00150.
[3]汪凯华，王学军，缪亚梅，等. 优质鲜食大粒蚕豆通蚕鲜8号的选育和栽培要点[J].江苏农业科学，2013，41（11）：113-115.
[4]黄洁，王超，闫景彩，等. 蚕豆的饲用现状和饲用改良技术发展趋势[J]. 饲料工业，2017，38（10）：60-64.
[5]叶茵. 中国蚕豆学[M]. 北京：中国农业出版社，2003.
[6]LIZARAZO C I, LAMPI A M, LIU J W, et al. Nutritive quality and protein production from grain legumes in a boreal climate[J]. Journal of the Science of Food and Agriculture,2015, 95: 2053-2064.
[7]LONGOBARDI F, SACCO D, CASIELLO G, et al. Chemical profile of the carpino broad bean by conventional and innovative physicochemical analyses[J]. Journal of Food Quality,2015,38: 273-284.
[8]NEME K, BULTOSA G, BUSSA N. Nutrient and functional properties of composite flours processed from pregelatinised barley, sprouted faba bean and carrot flours[J]. International Journal of Food Science and Technology,2015, 50: 2375-2382.
[9]TURCO I, FERRETTI G, BACCHETTI T. Review of the health benefits of faba bean (Vicia faba L.) polyphenols[J]. Journal of Food & Nutrition Research,2016, 55: 283-293.
[10]徐东旭，姜翠棉，宗绪晓. 蚕豆种质资源形态标记遗传多样性分析[J].植物遗传资源学报，2010，11（4）：399-406.
[11]王海飞，关建平，马钰，等.中国蚕豆种质资源ISSR标记遗传多样性分析[J].作物学报，2011，37（4）：595-602.
[12]ZONG X X, LIU X J, GUAN J P, et al. Molecular variation among Chinese and global winter faba bean germplasm[J]. Theoretical and Applied Genetics, 2009,118: 971-978.
[13]ZONG X, REN J, GUAN J, et al. Molecular variation among Chinese and global germplasm in spring faba bean areas[J]. Plant Breeding, 2010, 129: 508-513.
[14]EL-ESAWI M A. SSR analysis of genetic diversity and structure of the germplasm of faba bean (Vicia faba L.)[J].Comptes Rendus Biologies, 2017, 340 (11/12):474-480.
[15]GL �瘙 塁 , DOGˇANLAR S, FRARY A. Relationship between geographical origin, seed size and genetic diversity in faba bean (Vicia faba L.) as revealed by SSR markers[J]. Molecular Genetics and Genomics, 2017, 292:991-999.
[16] 欧阳裕元，余东梅，杨梅. 蚕豆主要农艺性状与单株产量的相关及通径分析[J]. 江苏农业学报，2016，32（4）：763-768.
[17] 余莉，张时龙，李清超，等. 蚕豆产量及主要农艺性状的相关及灰色关联度分析[J]. 湖北农业科学，2017，56（1）：18-20.
[18]杨生华，刘荣，杨涛，等.蚕豆种质资源种子表型性状精准评价[J].中国蔬菜，2016（10）：32-40.
[19]李欢，陈惠查，阮仁超，等.贵州特色蚕豆种质资源主要农艺性状分析与综合评价[J].农技服务，2020，37（8）：74-76.
[20]陈宏伟，李莉，刘昌燕，等. 129份湖北蚕豆地方种质的子粒外观及品质性状分析[J]. 湖北农业科学，2016，24（55）：6377-6380.
[21]康智明，郑开斌，徐晓俞，等. 不同蚕豆品种农艺及品质性状的遗传多样性分析[J]. 福建农业学报，2015，30（3）：249-252.
[22]石建斌，侯万伟，刘玉皎，等. 蚕豆种质资源清蛋白遗传多样性分析[J]. 植物遗传资源学报，2012，13（1）：22-28.
[23]LUCAS M R, HUYNH B L, VINHOLES P DA S,et al. Association studies and legume synteny reveal haplotypes determining seed size in Vigna unguiculata[J]. Frontiers in Plant Science, 2013, 4: 95.
[24]李鸿雁. 扁蓿豆种质资源遗传多样性的研究[D]. 呼和浩特：内蒙古农业大学，2008.
[25]吕伟，韩俊梅，文飞，等. 不同来源芝麻种质资源的表型多样性分析[J]. 植物遗传资源学报，2020，21（1）：234-242.
[26]张斌斌，蔡志翔，沈志军，等. 观赏桃种质资源表型性状多样性评价[J].中国农业科学，2021，54（11）：2406-2418.
[27]万述伟，宋凤景，郝俊杰，等. 271份豌豆种质资源农艺性状遗传多样性分析[J]. 植物遗传资源学报，2017，18（1）：10-18.
[28]杨涛，黄雅婕，李生梅，等. 海岛棉种质资源表型性状的遗传多样性分析及综合评价[J].中国农业科学，2021，54（12）：2499-2509.
[29]王姣姣.冷季豆品质性状近红外模型建立及区域分析[D]. 北京：中国农业科学院，2013.
[30] GICZEWSKA A, BOROWSKA J. Nutritional value of broad bean seeds. Part 3: changes of dietary fibre and starch in the production of commercial flours[J]. Food/Nahrung, 2004, 48(2): 116-122.
[31] 郝曦煜，杨涛，梁杰，等.160份外引鹰嘴豆种质主要农艺性状的遗传多样性分析[J].植物遗传资源学报，2020，21（4）：875-883.
[32] 王兰芬，武晶，王昭礼，等. 普通菜豆种质资源表型鉴定及多样性分析[J].植物遗传资源学报，2016，17（6）：976-983.
[33]李辉玲,张国儒,曾卫东,等. 基于InDel标记的新疆色素辣椒遗传多样性及聚类分析[J].江苏农业科学,2021,49(17):67-71.
[34]季琳琳,陈素传,吴志辉,等. 山核桃果实主要经济性状和养分含量的差异分析[J].南京林业大学学报(自然科学版),2022,46(1):131-137.
[35]谢薇,赖剑雄,秦晓威,等. 54份可可种质资源主要品质性状及相关分析[J].南方农业学报,2021,52(8):2174-2182.
[36]王治会,岳翠男,李琛,等. 江西省茶树种质化学特性多样性分析与鉴定评价[J].江苏农业学报,2020,36(1):172-179.
[37]彭枫，赵孟良，徐晨曦，等. 基于表型性状的菠菜种质资源遗传多样性分析[J]. 分子植物育种，2021，19（5）：1698-1708.

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备注/Memo

备注/Memo:
收稿日期：2021-10-24基金项目：国家食用豆产业技术体系建设项目（CARS-08-Z10）；江苏省现代农业产业技术体系建设项目；南通市科技计划项目（MS22020032）作者简介：赵娜（1986-），女，山东枣庄人，硕士，助理研究员，主要从事豆类遗传育种研究。(E-mail) zhaona5670@163.com通讯作者：王学军，(E-mail) wangxj4002@sina.com
更新日期/Last Update: 2022-07-07