[1]赵旭庭,徐盼,马政,等.枫泾猪、梅山猪和沙乌头猪全基因组ROH检测及候选基因鉴定[J].江苏农业学报,2021,(05):1234-1243.[doi:doi:10.3969/j.issn.1000-4440.2021.05.019]
　ZHAO Xu-ting,XU Pan,MA Zheng,et al.Genome-wide scan for run of homozygosity and identification of corresponding candidate genes in Fengjing pigs, Meishan pigs and Shawutou pigs[J].,2021,(05):1234-1243.[doi:doi:10.3969/j.issn.1000-4440.2021.05.019]
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枫泾猪、梅山猪和沙乌头猪全基因组ROH检测及候选基因鉴定()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年05期
页码:
1234-1243
栏目:
畜牧兽医·水产养殖
出版日期:
2021-10-30

文章信息/Info

Title:
Genome-wide scan for run of homozygosity and identification of corresponding candidate genes in Fengjing pigs, Meishan pigs and Shawutou pigs
作者:
赵旭庭1; 徐盼1; 马政1; 仲德1; 刘林雨1; 倪黎纲1; 吴井生2
（1.江苏农牧科技职业学院，江苏泰州225300；2.江苏农林职业技术学院，江苏句容212400）
Author(s):
ZHAO Xu-ting1; XU Pan1; MA Zheng1; ZHONG De1; LIU Lin-yu1; NI Li-gang1; WU Jing-sheng2
(1.Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China;2.Jiangsu Vocational College of Agriculture and Forestry, Jurong 212400, China)
关键词:
猪; 长纯合片段; 近交系数; 候选基因
Keywords:
pig; runs of homozygosity (ROH); inbreeding coefficient; candidate gene
分类号:
S813.1
DOI:
doi:10.3969/j.issn.1000-4440.2021.05.019
文献标志码:
A
摘要:
通过检测枫泾猪、梅山猪和沙乌头猪的长纯合片段（Runs of homozygosity, ROH），鉴定其与3个猪种经济性状相关的候选基因。利用全基因组单核苷酸多态性（Single Nucleotide Polymorphisms, SNP）芯片数据对这3个猪群体进行全基因组ROH检测，统计ROH数量、长度及分布，计算基于ROH的近交系数（FROH），并在高频ROH区域鉴定候选功能基因。结果显示，从枫泾猪、梅山猪和沙乌头猪群体中分别检测到1 588个、3 314个和4 419个ROH。枫泾猪的ROH平均长度为12.84 Mb，平均FROH为0.084；梅山猪的ROH平均长度为10.55 Mb，平均FROH为0.164；沙乌头猪的ROH平均长度为10.52 Mb，平均FROH为0.141。在高频ROH区域共鉴定到9个枫泾猪候选功能基因和8个梅山猪候选功能基因。研究结果为枫泾猪、梅山猪和沙乌头猪的资源保护和分子标记辅助选择奠定了基础。
Abstract:
By detecting runs of homozygosity (ROH) of Fengjing pig, Meishan pig and Shawutou pig, the candidate genes related to the economic traits of the three pig breeds were identified. The genome-wide single nucleotide polymorphisms (SNP) chip data were used to perform genome-wide ROH scan for the three pig breeds. The number, length and distribution of ROH were counted, and the inbreeding coefficient (FROH) was calculated based on ROH. In addition, the functional candidate genes were identified in the high frequency ROH region. The results showed that 1 588, 3 314 and 4 419 ROH were detected in the three pig populations, respectively. The average length of ROH was 12.84 Mb and the average FROH was 0.084 in Fengjing pigs. The average length of ROH was 10.55 Mb and the average FROH was 0.164 in Meishan pigs. The average length of ROH was 10.52 Mb and the average FROH was 0.141 in Shawutou pigs. Nine functional candidate genes of Fengjing pig and eight functional candidate genes of Meishan pig were identified in high frequency ROH region. The results lay a foundation for resource conservation and molecular marker-assisted selection of Fengjing pig, Meishan pig and Shawutou pig.

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备注/Memo

备注/Memo:
收稿日期：2021-02-07基金项目：国家自然科学基金（31702089）；江苏现代农业（生猪）产业技术体系[JATS(2020)346]；江苏高校“青蓝工程”项目作者简介：赵旭庭（1966-），男，江苏靖江人，硕士，教授，主要从事地方猪品种资源保护与遗传育种研究。(E-mail)jsfdyc@163.com。徐盼为共同第一作者。通讯作者：吴井生，(E-mail)17190607@qq.com
更新日期/Last Update: 2021-11-09